Fixing of bacteria to a slide is done by passing the bacterial smear over the flame of a Bunsen burner BUT it can also be chemically done using

methanol

What type of information below cannot be determined by a simple stain?
a. thick/thinness of a cell wall
b. cell arrangement
c. morphology
d. size

thick/thinness of cell wall

True or false. When making a smear from solid media you must mix your bacteria in a small amount of water first.

true

What is the name of the mordant in Gram-staining called?

Gram's iodine

Gram negative cells will show the color of the ____ stain after gram staining

safranin

What is the name of the apparatus you use in class to sterilize your inoculating tools?

Bunsen burner

There are 2 dilution techniques. Which technique does not allow you to quantify?
a. pour plate
b. streak plate
c. spread plate

streak plate

__________ is the most common isolation technique.

streak plate technique

After Gram-staining cells appear pink. What does this tell you about the bacterial cells?

the cells have a thin wall

The ideal age that a bacterial culture should be used for staining is?

18-24 hours

In the spread plate method colonies grow on and in the agar or only on the surface?

only on the surface

Turbidity can be used using a machine called a ______________.

spectrophotometer

What happens to the % of transmittance as a microbial cell population in nutrient broth increases?

decreases

What happens to absorbance as a microbial cell population in nutrient broth increases?

increases

In determining bacterial level cells/ml you should choose an agar plate that contains between _________ colonies.

25-250

Generation time is the time required for a ___________?

cell to divide into 2 cells

In Gram staining the safranin is the __________.

basic stain

The shorter the generation time the ________ the growth.

faster

A chromophore is the _____________.

the ion that is colored

A pure culture is one that?

only contains a single kind of microbe

The iris diaphragm can be found _______ the __________.

below the condenser

In a Brightfield microscope

dark objects are viewed against a bright background

In a parfocal microscope where the specimen has been focused at low power will only need minor adjustment by using the __________ as you increase the magnification.

fine adjustment knob

If the total magnification of your specimen is 400x which objective lens was being used?

high dry lens

What happens to the field of vision as you change your objective lens from low power to the scanning lens?

it increases

Agar is a solidifying agent that is extracted from?

algae

Which of the following type of media is used to grow bacteria that require small amounts of oxygen?

agar deep

A bacterial organism was introduces into a tube of nutrient broth which has been allowed to sit at 30*C for 24 hours. After this time it is observed to have clumps of microbial cells suspended in the broth while the remainder appears cloudy. What bacterial growth pattern is this exhibiting?

flocculent

Which of the following is solid media?
a. nutrient broth
b. Agar slant
c. Agar deep

agar slant

Permanent bacteria that can reside and multiply are

normal microflora

unwanted microbe

contaminant

liquid media is?

nutrient broth

cloudy as a result of bacterial growth is?

turbidity

intentional introduction of microbes onto or into media

inoculate

steam sterilization under pressure

autoclaving

population of cells arising from a single bacterial cell

colony

to grow

culture

substance that turned red when metabolized by bacteria

tetrazolium chloride

bacteria that reside temporarily and cannot multiply

transient

What tool should be used to introduce microbes into an agar deep?

inoculating needle