Fixing of bacteria to a slide is done by passing the bacterial smear over the flame of a Bunsen burner BUT it can also be chemically done using
methanol
What type of information below cannot be determined by a simple stain?
a. thick/thinness of a cell wall
b. cell arrangement
c. morphology
d. size
thick/thinness of cell wall
True or false. When making a smear from solid media you must mix your bacteria in a small amount of water first.
true
What is the name of the mordant in Gram-staining called?
Gram's iodine
Gram negative cells will show the color of the ____ stain after gram staining
safranin
What is the name of the apparatus you use in class to sterilize your inoculating tools?
Bunsen burner
There are 2 dilution techniques. Which technique does not allow you to quantify?
a. pour plate
b. streak plate
c. spread plate
streak plate
__________ is the most common isolation technique.
streak plate technique
After Gram-staining cells appear pink. What does this tell you about the bacterial cells?
the cells have a thin wall
The ideal age that a bacterial culture should be used for staining is?
18-24 hours
In the spread plate method colonies grow on and in the agar or only on the surface?
only on the surface
Turbidity can be used using a machine called a ______________.
spectrophotometer
What happens to the % of transmittance as a microbial cell population in nutrient broth increases?
decreases
What happens to absorbance as a microbial cell population in nutrient broth increases?
increases
In determining bacterial level cells/ml you should choose an agar plate that contains between _________ colonies.
25-250
Generation time is the time required for a ___________?
cell to divide into 2 cells
In Gram staining the safranin is the __________.
basic stain
The shorter the generation time the ________ the growth.
faster
A chromophore is the _____________.
the ion that is colored
A pure culture is one that?
only contains a single kind of microbe
The iris diaphragm can be found _______ the __________.
below the condenser
In a Brightfield microscope
dark objects are viewed against a bright background
In a parfocal microscope where the specimen has been focused at low power will only need minor adjustment by using the __________ as you increase the magnification.
fine adjustment knob
If the total magnification of your specimen is 400x which objective lens was being used?
high dry lens
What happens to the field of vision as you change your objective lens from low power to the scanning lens?
it increases
Agar is a solidifying agent that is extracted from?
algae
Which of the following type of media is used to grow bacteria that require small amounts of oxygen?
agar deep
A bacterial organism was introduces into a tube of nutrient broth which has been allowed to sit at 30*C for 24 hours. After this time it is observed to have clumps of microbial cells suspended in the broth while the remainder appears cloudy. What bacterial growth pattern is this exhibiting?
flocculent
Which of the following is solid media?
a. nutrient broth
b. Agar slant
c. Agar deep
agar slant
Permanent bacteria that can reside and multiply are
normal microflora
unwanted microbe
contaminant
liquid media is?
nutrient broth
cloudy as a result of bacterial growth is?
turbidity
intentional introduction of microbes onto or into media
inoculate
steam sterilization under pressure
autoclaving
population of cells arising from a single bacterial cell
colony
to grow
culture
substance that turned red when metabolized by bacteria
tetrazolium chloride
bacteria that reside temporarily and cannot multiply
transient
What tool should be used to introduce microbes into an agar deep?
inoculating needle