front 1 Fixing of bacteria to a slide is done by passing the bacterial smear over the flame of a Bunsen burner BUT it can also be chemically done using | back 1 methanol |
front 2 What type of information below cannot be determined by a simple stain?
| back 2 thick/thinness of cell wall |
front 3 True or false. When making a smear from solid media you must mix your bacteria in a small amount of water first. | back 3 true |
front 4 What is the name of the mordant in Gram-staining called? | back 4 Gram's iodine |
front 5 Gram negative cells will show the color of the ____ stain after gram staining | back 5 safranin |
front 6 What is the name of the apparatus you use in class to sterilize your inoculating tools? | back 6 Bunsen burner |
front 7 There are 2 dilution techniques. Which technique does not allow you to quantify?
| back 7 streak plate |
front 8 __________ is the most common isolation technique. | back 8 streak plate technique |
front 9 After Gram-staining cells appear pink. What does this tell you about the bacterial cells? | back 9 the cells have a thin wall |
front 10 The ideal age that a bacterial culture should be used for staining is? | back 10 18-24 hours |
front 11 In the spread plate method colonies grow on and in the agar or only on the surface? | back 11 only on the surface |
front 12 Turbidity can be used using a machine called a ______________. | back 12 spectrophotometer |
front 13 What happens to the % of transmittance as a microbial cell population in nutrient broth increases? | back 13 decreases |
front 14 What happens to absorbance as a microbial cell population in nutrient broth increases? | back 14 increases |
front 15 In determining bacterial level cells/ml you should choose an agar plate that contains between _________ colonies. | back 15 25-250 |
front 16 Generation time is the time required for a ___________? | back 16 cell to divide into 2 cells |
front 17 In Gram staining the safranin is the __________. | back 17 basic stain |
front 18 The shorter the generation time the ________ the growth. | back 18 faster |
front 19 A chromophore is the _____________. | back 19 the ion that is colored |
front 20 A pure culture is one that? | back 20 only contains a single kind of microbe |
front 21 The iris diaphragm can be found _______ the __________. | back 21 below the condenser |
front 22 In a Brightfield microscope | back 22 dark objects are viewed against a bright background |
front 23 In a parfocal microscope where the specimen has been focused at low power will only need minor adjustment by using the __________ as you increase the magnification. | back 23 fine adjustment knob |
front 24 If the total magnification of your specimen is 400x which objective lens was being used? | back 24 high dry lens |
front 25 What happens to the field of vision as you change your objective lens from low power to the scanning lens? | back 25 it increases |
front 26 Agar is a solidifying agent that is extracted from? | back 26 algae |
front 27 Which of the following type of media is used to grow bacteria that require small amounts of oxygen? | back 27 agar deep |
front 28 A bacterial organism was introduces into a tube of nutrient broth which has been allowed to sit at 30*C for 24 hours. After this time it is observed to have clumps of microbial cells suspended in the broth while the remainder appears cloudy. What bacterial growth pattern is this exhibiting? | back 28 flocculent |
front 29 Which of the following is solid media?
| back 29 agar slant |
front 30 Permanent bacteria that can reside and multiply are | back 30 normal microflora |
front 31 unwanted microbe | back 31 contaminant |
front 32 liquid media is? | back 32 nutrient broth |
front 33 cloudy as a result of bacterial growth is? | back 33 turbidity |
front 34 intentional introduction of microbes onto or into media | back 34 inoculate |
front 35 steam sterilization under pressure | back 35 autoclaving |
front 36 population of cells arising from a single bacterial cell | back 36 colony |
front 37 to grow | back 37 culture |
front 38 substance that turned red when metabolized by bacteria | back 38 tetrazolium chloride |
front 39 bacteria that reside temporarily and cannot multiply | back 39 transient |
front 40 What tool should be used to introduce microbes into an agar deep? | back 40 inoculating needle |