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Biology Lab II Quiz 1

front 1

Why are open reading frames important, what do they indicate, and how many are there on single-stranded DNA?

back 1

part of a reading frame that contain no stop codons, sequence of nucleotide triplets read as codons specifying amino acids, a single strand of DNA has THREE possible reading frames, long ORFs indicate candidate coding regions

front 2

What are the programs and databases are used to annotate, and what does each program and database tell us

back 2

DNA Master – our genome editor

Glimmer, GeneMark (Starts/Stops)

NCBI, PhagesDB, HHPred (Blast data)

Phamerator, Starterator (Comparative data)

front 3

What is the difference between a gene and gene product?

back 3

a gene is a fundamental unit of heredity, a gene product is biochemical material produced as a result of gene expression

front 4

What is the difference between a DNA sequence and gene product sequence?

back 4

DNA Sequence: nucleotides

Gene Product: amino acids

front 5

Why is synteny important in the annotation process?

back 5

framework for identifying conserved homologous genes and their gene order between genomes of different species

front 6

STOP CODONS!

back 6

TAG, TGA, TAA

front 7

START CODONS!

back 7

ATG, GTG, TTG