Why are open reading frames important, what do they indicate, and how many are there on single-stranded DNA?
part of a reading frame that contain no stop codons, sequence of nucleotide triplets read as codons specifying amino acids, a single strand of DNA has THREE possible reading frames, long ORFs indicate candidate coding regions
What are the programs and databases are used to annotate, and what does each program and database tell us
DNA Master – our genome editor
Glimmer, GeneMark (Starts/Stops)
NCBI, PhagesDB, HHPred (Blast data)
Phamerator, Starterator (Comparative data)
What is the difference between a gene and gene product?
a gene is a fundamental unit of heredity, a gene product is biochemical material produced as a result of gene expression
What is the difference between a DNA sequence and gene product sequence?
DNA Sequence: nucleotides
Gene Product: amino acids
Why is synteny important in the annotation process?
framework for identifying conserved homologous genes and their gene order between genomes of different species
STOP CODONS!
TAG, TGA, TAA
START CODONS!
ATG, GTG, TTG