week 11 final lab
1. Penicillin is made by a fungus and used to kill bacterial cells. Therefore, penicillin is an example of a(n)
antibiotic
An antimicrobial that is effective against many types of pathogenic bacteria would be considered a_____agent
Broad spectrum
what information does Kirby Bauer method provide
Sensitivity and resistance to antibiotics
what type of medium is used in the Kirby Bauer Method
Mueller Hinton
What tool is usually used for inoculating the agar when creating a bacterial lawn
sterile swab
After inoculating in this way, how will the plate appear after inoculation?
Non isolated bacterial colonies all over the surface
What are the disks used for in this method
They contain different antibiotics
True or false if the zone of inhibition is large, the organism must be sensitive to the anibiotic
false
When testing for synergism of antibiotics, how many different disks are applied to the bacterial lawn?
2
7. What does synergism mean?
The effectiveness of both antibiotics is better than either antibiotic alone
In a Kirby-Bauer antimicrobial susceptibility test, the antimicrobial disk with the largest zone of inhibition is the one that the test organism is most sensitive to.
False
If the Mueller-Hinton agar plate used for the Kirby-Bauer test is too thin, what effect will this have on the diameter of the zones of inhibition?
They will be larger they would be on a plate of the appropriate thickness
If you test ten different gram-positive and gram-negative organisms and find that almost all of them are sensitive to a particular drug, what would be that drug's spectrum of activity?
Broad spectrum
Please choose the method of antimicrobial susceptibility testing that involves measuring and evaluating zones of inhibition around antibiotic disks placed on a culture of bacteria prior to incubation.
Kirby Bauer test
A chemical compound, regardless of its source (either natural or synthetic), that is capable of killing or inhibiting microorganisms is referred to as
an antimicrobial
Which choice below briefly describes the Kirby-Bauer method of antimicrobic sensitivity testing?
disk diffusion