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50 notecards = 13 pages (4 cards per page)

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Microbiology lab exam

front 1

parts of the microscope

back 1

front 2

cocci

back 2

round shape

front 3

diplococci

back 3

cocci that remain in pairs after dividing

front 4

streptococci

back 4

divides and remain attached in chainlike

front 5

tetrads

back 5

two planes and remain in groups of four

front 6

staphylococci

back 6

multiple planes. grapelike

front 7

bacilli

back 7

rodlike

front 8

single bacilli

back 8

single rods

front 9

diplobacilli

back 9

pairs after division

front 10

steptobacilli

back 10

chains

front 11

coccobacillus

back 11

oval like cocci

front 12

vibrio

back 12

curved rods. like a comma

front 13

spirillum

back 13

helical shape

front 14

spirochete

back 14

helical and flexible

front 15

spore staining

back 15

prepare slide

add culture

air dry (10min)

heat dry (5X)

put on noiling water

flood slide with malachite green

remove after 10mins and rinse with DI water

add safranin

wait 1min

perform microscopy

front 16

E.coli

back 16

gram negative

non acid fast

non sporulating

acid slant and acid butt

front 17

S. aureus

back 17

gram positive

non acid fast

non sporulating

front 18

bacillus

back 18

gram positive

acid fast

sporulating

front 19

M. smeg

back 19

acid fast

front 20

what test do you perform when a microbe is found to be catalase positive

back 20

coagulase test

DNAase

MSA

front 21

What happens to a medium when H2O2 is added to it

back 21

it bubbles which shows that it's catalase positive

front 22

which organisms came out to be catalase positive

back 22

S. aureus and S. epidermis

front 23

which organisms came out to be catalase negative

back 23

S.pyogenes

S. salvarius

S. fecalis

front 24

Which organism was MSA positive and what color did it turn

back 24

S. aureus; yellow

front 25

which organism was MSA negative and what color did it turn

back 25

S. epidermis; red

front 26

which organism had a clear halo after the DNAase test

back 26

S. aureus

front 27

which organism had no halo after the DNAase test

back 27

S. epidermis

front 28

Which organism clotted for the coagulase test

back 28

S. aureus

front 29

which organism didn't clot for the coagulase test

back 29

S. epidermis

front 30

Which organism turned black for the bile esculin test

back 30

S. fecalis

front 31

which organism turned green for the bile esculin test

back 31

S. pyogenes

S. salvaris

front 32

which organism turned yellow for the phenol red test

back 32

S. fecalis

front 33

which organism turned red for the phenol red test

back 33

s. pyogenes

S. salvarius

front 34

For the blood hemolysis test, which one was gamma

back 34

S. fecalis

front 35

which blood hemolysis came out to be beta

back 35

S. pyogenes

front 36

which blood hemolysis came out to be alpha

back 36

S. salvarius

front 37

what color does the organism turn when it's lactose fermenter

back 37

pink

front 38

what color does the organism turn when it's a non-lactose fermenter

back 38

colorless/white

front 39

what dye is first used in a gram staining

back 39

crystal violet followed by acetone decolorizer, then safranin

front 40

what dye is used in spore staining

back 40

malachite green

front 41

what was added to the TSI tubes

back 41

kovac's reagant

front 42

P. aer

back 42

pinkish

alkaline slant

alkaline butt

non lactose

front 43

P. vul

back 43

yellow slant

yellow butt

gas inside

black ppt

front 44

C. fir

back 44

black ppt

creates gas

yellow slant

front 45

K. pne

back 45

lactose fermenter

yellow everywhere

front 46

S. ent

back 46

black ppt only in acid

it turns the media yellow first

front 47

mordent for gram staining technique

back 47

iodine and moist heat

front 48

what dye is used as a counter stain

back 48

methylene blue

front 49

decolorizer for acid fast stain?

back 49

acid alcohol

front 50

decolorizer in gram staining

back 50

acetone alcohol