parts of the microscope
cocci
round shape
diplococci
cocci that remain in pairs after dividing
streptococci
divides and remain attached in chainlike
tetrads
two planes and remain in groups of four
staphylococci
multiple planes. grapelike
bacilli
rodlike
single bacilli
single rods
diplobacilli
pairs after division
steptobacilli
chains
coccobacillus
oval like cocci
vibrio
curved rods. like a comma
spirillum
helical shape
spirochete
helical and flexible
spore staining
prepare slide
add culture
air dry (10min)
heat dry (5X)
put on noiling water
flood slide with malachite green
remove after 10mins and rinse with DI water
add safranin
wait 1min
perform microscopy
E.coli
gram negative
non acid fast
non sporulating
acid slant and acid butt
S. aureus
gram positive
non acid fast
non sporulating
bacillus
gram positive
acid fast
sporulating
M. smeg
acid fast
what test do you perform when a microbe is found to be catalase positive
coagulase test
DNAase
MSA
What happens to a medium when H2O2 is added to it
it bubbles which shows that it's catalase positive
which organisms came out to be catalase positive
S. aureus and S. epidermis
which organisms came out to be catalase negative
S.pyogenes
S. salvarius
S. fecalis
Which organism was MSA positive and what color did it turn
S. aureus; yellow
which organism was MSA negative and what color did it turn
S. epidermis; red
which organism had a clear halo after the DNAase test
S. aureus
which organism had no halo after the DNAase test
S. epidermis
Which organism clotted for the coagulase test
S. aureus
which organism didn't clot for the coagulase test
S. epidermis
Which organism turned black for the bile esculin test
S. fecalis
which organism turned green for the bile esculin test
S. pyogenes
S. salvaris
which organism turned yellow for the phenol red test
S. fecalis
which organism turned red for the phenol red test
s. pyogenes
S. salvarius
For the blood hemolysis test, which one was gamma
S. fecalis
which blood hemolysis came out to be beta
S. pyogenes
which blood hemolysis came out to be alpha
S. salvarius
what color does the organism turn when it's lactose fermenter
pink
what color does the organism turn when it's a non-lactose fermenter
colorless/white
what dye is first used in a gram staining
crystal violet followed by acetone decolorizer, then safranin
what dye is used in spore staining
malachite green
what was added to the TSI tubes
kovac's reagant
P. aer
pinkish
alkaline slant
alkaline butt
non lactose
P. vul
yellow slant
yellow butt
gas inside
black ppt
C. fir
black ppt
creates gas
yellow slant
K. pne
lactose fermenter
yellow everywhere
S. ent
black ppt only in acid
it turns the media yellow first
mordent for gram staining technique
iodine and moist heat
what dye is used as a counter stain
methylene blue
decolorizer for acid fast stain?
acid alcohol
decolorizer in gram staining
acetone alcohol