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46 notecards = 12 pages (4 cards per page)

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Selective/Differential Media

front 1

Which types of media in this lab are selective for Gram+ organisms ?

back 1

Mannitol Salt Agar

front 2

on what basis are the Gram + selective media also differential?

back 2

for mannitol fermentation

front 3

Which types of media used in this lab are selective for Gram - organisms?

back 3

MacConkey agar and Eosin Methylene Blue

front 4

on what basis for the Gram- selective media also differential?

back 4

for lactose fermentation

front 5

what color is mannitol salt agar before it is inoculated

back 5

red

front 6

how can the appearance of MSA plate change with a positive differential result

back 6

color change to yellow

front 7

What color is MacConkey agar before it is inoculated?

back 7

Raspberry

front 8

How is the appearance of MAC plate change with a positive differential result?

back 8

pink with precipitate

front 9

what color is eosin methylene blue agar before it is inoculated?

back 9

burgundy purple

front 10

how is the appearance of EMB plate change with a positive differential result ?

back 10

shiny metallic green

front 11

What is the definition of a selective medium?

back 11

grow only certain types of organisms and actively preventing some types from growing

front 12

what is the definition of a differential medium?

back 12

grow all types of microbes but it causes some organisms to look different

front 13

Which type medium used in lab so far this semester is neither selective nor differential?

back 13

Tryptic Soy Agar

front 14

what is the purpose of differential media ?

back 14

for identification

front 15

what is the purpose of selective media ?

back 15

for identification

front 16

grow all types of microorganisms but it causes some organisms to look different

back 16

differential media

front 17

grow only certain types of organisms actively preventing some types from growing

back 17

selective media

front 18

what is enrichment media

back 18

it is designed to promote the growth of a rare organism

front 19

designed to promote the growth of rare organisms

back 19

enrichment media

front 20

which bacteria would it be if the eosin methylene blue plate is shiny metallic green?

back 20

Escherichia coli

front 21

back 21

Escherichia coli

front 22

which bacteria would it be if in MacConkey agar is pink with precipitate ?

back 22

Escherichia coli

front 23

back 23

Escherichia coli

front 24

back 24

Enterobacter aerogenes

front 25

which bacteria would it be in eosin methylene blue if it were pink and moist ?

back 25

Enterobacter aerogenes

front 26

what bacteria would it be in MacConkey of it were pink without precipitate ?

back 26

Enterobacter aerogenes

front 27

back 27

Enterobacter aerogenes

front 28

back 28

Proteus vulgaris

front 29

what bacteria would it be on a MacConkey agar and it were no new color on plate and is brownish

back 29

Proteus vulgaris

front 30

back 30

Proteus vulgaris

front 31

What is the bacteria eosin methylene blue if there is no color change?

back 31

Proteus vulgaris

front 32

strong lactose fermenter

back 32

Escherichia coli

front 33

medium lactose fermenter

back 33

Enterobacter aerogenes

front 34

does not ferment lactose

back 34

Proteus vulgaris

front 35

back 35

Staphylococcus aureus

front 36

which bacteria would be on a mannitol salt agar if it were yellow

back 36

Staphylococcus aureus

front 37

back 37

Staphylococcus epidermidis

front 38

what bacteria would it be if mannitol salt agar remains red, and maybe more pink?

back 38

Staphylococcus epidermidis

front 39

weak mannitol fermenter

back 39

Staphylococcus epidermidis

front 40

strong mannitol fermenter

back 40

Staphylococcus aureus

front 41

EMB selective

back 41

against Gram +, for Gram -

front 42

MAC selective

back 42

against Gram +, for Gram -

front 43

EMB differential

back 43

lactose fermentation

front 44

MAC differential

back 44

lactose fermentation

front 45

MSA selective

back 45

against Gram -, for Gram +

front 46

MSA differential

back 46

mannitol fermentation