Which types of media in this lab are selective for Gram+ organisms ?
Mannitol Salt Agar
on what basis are the Gram + selective media also differential?
for mannitol fermentation
Which types of media used in this lab are selective for Gram - organisms?
MacConkey agar and Eosin Methylene Blue
on what basis for the Gram- selective media also differential?
for lactose fermentation
what color is mannitol salt agar before it is inoculated
red
how can the appearance of MSA plate change with a positive differential result
color change to yellow
What color is MacConkey agar before it is inoculated?
Raspberry
How is the appearance of MAC plate change with a positive differential result?
pink with precipitate
what color is eosin methylene blue agar before it is inoculated?
burgundy purple
how is the appearance of EMB plate change with a positive differential result ?
shiny metallic green
What is the definition of a selective medium?
grow only certain types of organisms and actively preventing some types from growing
what is the definition of a differential medium?
grow all types of microbes but it causes some organisms to look different
Which type medium used in lab so far this semester is neither selective nor differential?
Tryptic Soy Agar
what is the purpose of differential media ?
for identification
what is the purpose of selective media ?
for identification
grow all types of microorganisms but it causes some organisms to look different
differential media
grow only certain types of organisms actively preventing some types from growing
selective media
what is enrichment media
it is designed to promote the growth of a rare organism
designed to promote the growth of rare organisms
enrichment media
which bacteria would it be if the eosin methylene blue plate is shiny metallic green?
Escherichia coli
Escherichia coli
which bacteria would it be if in MacConkey agar is pink with precipitate ?
Escherichia coli
Escherichia coli
Enterobacter aerogenes
which bacteria would it be in eosin methylene blue if it were pink and moist ?
Enterobacter aerogenes
what bacteria would it be in MacConkey of it were pink without precipitate ?
Enterobacter aerogenes
Enterobacter aerogenes
Proteus vulgaris
what bacteria would it be on a MacConkey agar and it were no new color on plate and is brownish
Proteus vulgaris
Proteus vulgaris
What is the bacteria eosin methylene blue if there is no color change?
Proteus vulgaris
strong lactose fermenter
Escherichia coli
medium lactose fermenter
Enterobacter aerogenes
does not ferment lactose
Proteus vulgaris
Staphylococcus aureus
which bacteria would be on a mannitol salt agar if it were yellow
Staphylococcus aureus
Staphylococcus epidermidis
what bacteria would it be if mannitol salt agar remains red, and maybe more pink?
Staphylococcus epidermidis
weak mannitol fermenter
Staphylococcus epidermidis
strong mannitol fermenter
Staphylococcus aureus
EMB selective
against Gram +, for Gram -
MAC selective
against Gram +, for Gram -
EMB differential
lactose fermentation
MAC differential
lactose fermentation
MSA selective
against Gram -, for Gram +
MSA differential
mannitol fermentation