front 1 The reaction catalyzed by reverse transcriptase is | back 1 mRNA - cDNA |
front 2 Advantage of using E coli to make a human gene product | back 2 its genes are well known |
front 3 Which of the following is an agricultural product made by DNA techniques? | back 3 a) frost retardant b) Bacillus thuringiensis insecticide c) nitrogen fixation d) glyphosate resistant crops |
front 4 if you have inserted a gen in the Ti plasmid, the next step is | back 4 inserting the Ti plasmid into Agrobacterium |
front 5 Biotechnology involves the | back 5 a) use of microorganisms t make desired products, the use of animal cells t make vaccines, and the development of disease-resistant crop plants |
front 6 Which of the following is a desired characteristics of DNA vectors used in gene cloning procedures | back 6 a) self-replication b) has a selectable marker c) circular form of DNA d) replicate in several species |
front 7 An advantage of synthetic DNA is the ability to | back 7 insert desired restriction sites into the DNA sequence |
front 8 An advantage of cDNA is that it | back 8 lacks introns |
front 9 Genomic libraries contain | back 9 gene introns |
front 10 Pieces of DNA stored in yeast cell are called a | back 10 library |
front 11 A population of cells carrying a desired plasmid is called a | back 11 clone |
front 12 Self-replicating DNA used to transmit a gene from one organism to another is a | back 12 vector |
front 13 The Human Genome Project was focused on | back 13 determine the nucleotide sequence of the entire human genome |
front 14 The steps in the PCR procedure | back 14 1. Incubate at 94 C to denature DNA strands 2. Incubate at 60 C for primer hybridization 3. Incubate at 72 C for DNA synthesis |
front 15 Which of the following techniques are used to introduce recombinant DNA into plants? | back 15 a) gene guns b) protoplast fusion c) Ti plasmids and Agrobacterium d) electroporation |
front 16 PCR can be used to identify an unknown bacterium because | back 16 the RNA primer is specific |
front 17 The random shotgun method is used in | back 17 genome sequencing |
front 18 Restriction enzymes are | back 18 Bacterial enzymes that destroy phage DNA |
front 19 A vector is a self-replicating segment of DNA such as | back 19 plasmid or viral genome |
front 20 A shuttle vector is a plasmid that is used to move pieces of DNA among organisms such as bacterial, fungal and plant cells | back 20 True |
front 21 The Ti plasmid isolated from Agrobacterium can be used to insert DNA into any type of plant | back 21 False |
front 22 The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction | back 22 True |