The reaction catalyzed by reverse transcriptase is
mRNA - cDNA
Advantage of using E coli to make a human gene product
its genes are well known
Which of the following is an agricultural product made by DNA techniques?
a) frost retardant
b) Bacillus thuringiensis insecticide
c) nitrogen fixation
d) glyphosate resistant crops
if you have inserted a gen in the Ti plasmid, the next step is
inserting the Ti plasmid into Agrobacterium
Biotechnology involves the
a) use of microorganisms t make desired products, the use of animal cells t make vaccines, and the development of disease-resistant crop plants
Which of the following is a desired characteristics of DNA vectors used in gene cloning procedures
a) self-replication
b) has a selectable marker
c) circular form of DNA
d) replicate in several species
An advantage of synthetic DNA is the ability to
insert desired restriction sites into the DNA sequence
An advantage of cDNA is that it
lacks introns
Genomic libraries contain
gene introns
Pieces of DNA stored in yeast cell are called a
library
A population of cells carrying a desired plasmid is called a
clone
Self-replicating DNA used to transmit a gene from one organism to another is a
vector
The Human Genome Project was focused on
determine the nucleotide sequence of the entire human genome
The steps in the PCR procedure
1. Incubate at 94 C to denature DNA strands
2. Incubate at 60 C for primer hybridization
3. Incubate at 72 C for DNA synthesis
Which of the following techniques are used to introduce recombinant DNA into plants?
a) gene guns
b) protoplast fusion
c) Ti plasmids and Agrobacterium
d) electroporation
PCR can be used to identify an unknown bacterium because
the RNA primer is specific
The random shotgun method is used in
genome sequencing
Restriction enzymes are
Bacterial enzymes that destroy phage DNA
A vector is a self-replicating segment of DNA such as
plasmid or viral genome
A shuttle vector is a plasmid that is used to move pieces of DNA among organisms such as bacterial, fungal and plant cells
True
The Ti plasmid isolated from Agrobacterium can be used to insert DNA into any type of plant
False
The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction
True