front 1 What is the purpose of heat-fixing the smear? | back 1 to kill the organisms/bacteria and to attach them to the slide |
front 2 Which part of the specimen (cell or background) is stained in a Simple Stain? | back 2 the cell |
front 3 Would you have gotten the same result if you used an anionic (-charged) stain? | back 3 No |
front 4 Do you obtain an accurate estimate of cell size with a simple stain? | back 4 No |
front 5 For which step is the timing most critical? | back 5 the decolorizing step because over decolorization may occur |
front 6 What would you expect to observe if the Simple Stain procedure rather than the Gram stain had been used on a smear containing a mixture of gram-positive cocci and gram negative rods? | back 6 the cells would all be the same color |
front 7 Microorganisms are best defined as organisms that | back 7 are too small to be seen with the unaided eye |
front 8 Among the types of microorganisms, the ______ are noncellular. | back 8 Viruses |
front 9 Unable to obtain sharp focus using oil immersion objective lens. What is the possible cause? | back 9 Condenser is too low |
front 10 If a microbiology lab technician left the safranin out of the Gram stain procedure, what would be the result? | back 10 the Gram-positive cells would remain purple but the Gram-negative cells would be colorless |
front 11 A pure culture contains | back 11 only one identified species of microorganism |
front 12 What is the term for a culture made from one isolated colony? | back 12 Both axenic and pure |