What is the purpose of heat-fixing the smear?
to kill the organisms/bacteria and to attach them to the slide
Which part of the specimen (cell or background) is stained in a Simple Stain?
the cell
Would you have gotten the same result if you used an anionic (-charged) stain?
No
Do you obtain an accurate estimate of cell size with a simple stain?
No
For which step is the timing most critical?
the decolorizing step because over decolorization may occur
What would you expect to observe if the Simple Stain procedure rather than the Gram stain had been used on a smear containing a mixture of gram-positive cocci and gram negative rods?
the cells would all be the same color
Microorganisms are best defined as organisms that
are too small to be seen with the unaided eye
Among the types of microorganisms, the ______ are noncellular.
Viruses
Unable to obtain sharp focus using oil immersion objective lens. What is the possible cause?
Condenser is too low
If a microbiology lab technician left the safranin out of the Gram stain procedure, what would be the result?
the Gram-positive cells would remain purple but the Gram-negative cells would be colorless
A pure culture contains
only one identified species of microorganism
What is the term for a culture made from one isolated colony?
Both axenic and pure