Print Options

Card layout: ?

← Back to notecard set|Easy Notecards home page

Instructions for Side by Side Printing
  1. Print the notecards
  2. Fold each page in half along the solid vertical line
  3. Cut out the notecards by cutting along each horizontal dotted line
  4. Optional: Glue, tape or staple the ends of each notecard together
  1. Verify Front of pages is selected for Viewing and print the front of the notecards
  2. Select Back of pages for Viewing and print the back of the notecards
    NOTE: Since the back of the pages are printed in reverse order (last page is printed first), keep the pages in the same order as they were after Step 1. Also, be sure to feed the pages in the same direction as you did in Step 1.
  3. Cut out the notecards by cutting along each horizontal and vertical dotted line
To print: Ctrl+PPrint as a list

59 notecards = 15 pages (4 cards per page)

Viewing:

Histology(Ch1-5)

front 1

Where did Hematoxylin come from

back 1

From Logwood

front 2

Is hematoxylin an acid or a base

back 2

Base

front 3

What does Hematoxylin stain

back 3

RNA, DNA, Carbohydrates and cartilage, because they're acidic

front 4

What does eosin stain

back 4

Most proteins and some extracellular fibers

front 5

Is eosin an acid or a base

back 5

Negative Acid

front 6

What color does Hematoxylin stain the Nucleus

back 6

Purplish blue

front 7

What color does eosin stain

back 7

Red or pink

front 8

What are the compounds in Masson's trichrome

back 8

Hematoxylin, acid fuchsin, methyl blue

front 9

What color does Masson's trichrome stain Nuclei

back 9

Blue

front 10

What color does Masson's trichrome stain connective tissue and basement layers

back 10

Green

front 11

Whats Van Gieson stain used for?

back 11

For connective tissue detection

front 12

Whats Geimsa stains used for

back 12

Detecting Erythrocytes in the bone marrow to see if they're sick, and also for blood smears (is a test used to see the shape, size and appearance of red blood cells)

front 13

What color does giemsa stains stain red blood cells

back 13

Pink

front 14

What color does giemsa stains stain white blood cells

back 14

Dark purple/ blue

front 15

What does silver stain used for

back 15

To stain nerves

front 16

Whats Golgi-Cox used for

back 16

To stain nerves

front 17

What are the compounda in Golgi-Cox

back 17

Mercuric chloride, potassium chromate K2CrO4, potassium dichromate K2Cr2O7

front 18

Whats Cresyl violet used for

back 18

Detecting the endoplasmatic reticulum of the body of nerve cells(perikarya)

front 19

What color does Cresyl Violet stain body of neurons?

back 19

Brown

front 20

Name lipid stains

back 20

Nile Blue, Oil Red O and Sudan black

front 21

How thick is sectioning if done with microtome

back 21

10-20 Microns

front 22

What are low power Lenses

back 22

*1.6, *5 and *10

front 23

What are Hight low power Lenses

back 23

*20, *40, *63 and *100

front 24

What is the wave length range for halogen lamp(tungsten lamp)

back 24

starts at 300-400nm to 700-800nm

front 25

What does the magnification of Light microscope depend on?

back 25

the Magnification of the eye piece and Objective lens, for example if you had a eyepiece in a light microscope that can magnify by x10 and then objective lens that can magnify by x10 then in total your light microscope can magnify by x100

front 26

Whats the best resolution obtainable from a light microscope

back 26

0.2 Microns or 200nm

front 27

How big are cells in diameter

back 27

20-40 microns

front 28

Whats the Wave length in a Electron microscope

back 28

0.004nm

front 29

What does electro microscope use instead of lenses to focus the light

back 29

electromagnetic coils

front 30

What is the Numerical aperture for electron microscope

back 30

0.012

front 31

What is the greatest magnification for electron microscope

back 31

x50,000

front 32

How thick are the prepared sections for Electron microscope

back 32

60-100nm

front 33

How thick is the plasmatic membrane

back 33

8-10nm

front 34

How much is the diameter of the Nucleus

back 34

10 Microns

front 35

What is the function of the Nucleolus

back 35

Making Ribosomes

front 36

How much of cell volume do endoplasmic reticulum constitute

back 36

10%

front 37

What is the function of the endoplasmic reticulum

back 37

  • Store Calcium
  • make membranes for the other organelles(rough: makes the necessary protein, Smooth: makes the necessary lipids)

front 38

How many membranes does the Smooth ER have

back 38

it has the same structure as the plasmatic membrane, a single lipid bilayer membrane

front 39

How wide are the lumens of Rough ER

back 39

20-30nm

front 40

How wide are the lumens of Smooth ER

back 40

30-60nm

front 41

What is Cisternae

back 41

its the first 3-7 discs of the Golgi apparatus, the discs that receive, modify, transform and pack proteins

front 42

What is the trans face of Golgi apparatus

back 42

its the face that transports the proteins to the plasmatic membrane

front 43

What is the function of secreting vesicles

back 43

to get molecules to the plasmatic membrane

front 44

What is the function of Endosomes

back 44

they bring molecules from outside the cell inward, then to the lysosomes so that it can break it down

front 45

What is the function of Macropinosomes

back 45

they bring large amounts of fluid into the cell

front 46

What is the function of endosomes that do Phagocytosis

back 46

they kill bacteria

front 47

What is the function of Peroxisomes

back 47

they degrade fatty acids by adding an oxygen, then convert them to cholesterol

front 48

Where are Peroxisomes common/abundant

back 48

liver and kidney

front 49

How big are vesicles

back 49

50-200nm

front 50

What is Christae

back 50

they're the folds of the inner membrane of mitochondria that folds inward, the folds are empty intermediate space

front 51

What is the function of Actin filaments

back 51

  • Act as a track for Myosin
  • they link cells to each other by tight or adhesion junctions
  • they're an important component in the creation of cell protuberances(elevations on the surface of the cell membrane) like Microvilli in the intestine

front 52

How thick are actin filaments

back 52

7nm

front 53

What is the function of Intermediate filaments

back 53

they make it easier for cells to stick to each other by desmosomes and hemidesmosomes

front 54

What is the function of Microtubules

back 54

  • act as tracks for motors (kinesins, dynein) that move vesicles around the cell
  • they make the flagella and cilia
  • they make the mitotic and meiotic spindles

front 55

What makes Microtubules

back 55

Centrosomes

front 56

How big are Intermediate filaments

back 56

10nm

front 57

How big are Microtubules

back 57

25nm

front 58

What are the things you do in tissue processing for electron microscope that you don't do in light Microscopy

back 58

1)The fixative isn't formality It's rather glutaraldehyde

2)Post fixation(another fixation after glutaraldehyde) by osmic acid

3)We put the tissue in propylene oxide that allows us to impregnate it later with resin instead of wax

4) Machine For sectioning is Ultramicrotome That has a glass Or diamond knife

5)Stains are heavy salt Metals like Osmium, uranyl acetate And lead To increase. The contrast Because they dispersed

front 59

Why do we post-fixate with osmic acid in tissue processing for electron microscopy

back 59

glutaraldehyde results in cross-linking proteins that reserves protein structure while osmic acid preserves lipids