front 1 Remember lab safety | back 1 kk |
front 2 What do micropipettes do? | back 2 help us accurately and precisely transfer small volumes of liquid |
front 3 What are the pipette classifications? | back 3 P10 - 0.5 to 10 P100 - 10 to 100 P1000 - 100 to 1000 |
front 4 What is the milliliter to microliter conversion? | back 4 1ml to 1000ul |
front 5 What is the range of volume to be pipetted? | back 5 35%-100% |
front 6 True or false the pipette needs to be held upright when tip is filled with liquid | back 6 true |
front 7 The steps to use a micropipette | back 7 1. Set the volume on the pipette by turning the round part of the plunger. 2. Fit the proper tip on the micropipette 3. When bringing the liquid up the pipette stop at the first stop. 4. When dispensing the liquid push down past the first stop and to the second stop. 5. Finally eject the stop |
front 8 True or false. Tilt your micropipette after it has liquid in the tip | back 8 false |
front 9 What does gel electrophoresis do? | back 9 It is an efficient way to separate samples based on charge and weight. |
front 10 True or false. The substance moves from negative to positive | back 10 true |
front 11 What is the purpose of a centrifuge | back 11 They rotate at high speed to separate samples based on their density. |
front 12 True or false. It is important that centrifuges are balanced to work properly. | back 12 True (therefore you want to either place samples across from each other or at an axis) |
front 13 After being in the centrifuge what two parts of the substance is left | back 13 the liquid (supernatant) and the solid (pellet) |
front 14 What technique do you use to transfer microbes from a tube to a plate | back 14 aseptic technique |
front 15 What are the steps of transferring microbes | back 15 1. Use a tool (loop) and heat treat it with a Bunsen burner 2. Get a sample tube and treat it with the Bunsen burner too. 3. Take tool and contaminate with substance in sample tube 4. Use tool to spread substance on plate in a zig zag motion |