Remember lab safety
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What do micropipettes do?
help us accurately and precisely transfer small volumes of liquid
What are the pipette classifications?
P10 - 0.5 to 10
P100 - 10 to 100
P1000 - 100 to 1000
What is the milliliter to microliter conversion?
1ml to 1000ul
What is the range of volume to be pipetted?
35%-100%
True or false the pipette needs to be held upright when tip is filled with liquid
true
The steps to use a micropipette
1. Set the volume on the pipette by turning the round part of the plunger.
2. Fit the proper tip on the micropipette
3. When bringing the liquid up the pipette stop at the first stop.
4. When dispensing the liquid push down past the first stop and to the second stop.
5. Finally eject the stop
True or false. Tilt your micropipette after it has liquid in the tip
false
What does gel electrophoresis do?
It is an efficient way to separate samples based on charge and weight.
True or false. The substance moves from negative to positive
true
What is the purpose of a centrifuge
They rotate at high speed to separate samples based on their density.
True or false. It is important that centrifuges are balanced to work properly.
True (therefore you want to either place samples across from each other or at an axis)
After being in the centrifuge what two parts of the substance is left
the liquid (supernatant) and the solid (pellet)
What technique do you use to transfer microbes from a tube to a plate
aseptic technique
What are the steps of transferring microbes
1. Use a tool (loop) and heat treat it with a Bunsen burner
2. Get a sample tube and treat it with the Bunsen burner too.
3. Take tool and contaminate with substance in sample tube
4. Use tool to spread substance on plate in a zig zag motion