front 1 Which of the following is NOT considered a significant advance in PCR methodology? The invention of the thermal cycler The use of reverse transcriptase to generate cDNA from single-stranded RNA The use of agarose gel electrophoresis to visualize amplified products The use of fluorescently-tagged probes for real-time quantitation of PCR | back 1 The use of agarose gel electrophoresis to visualize amplified products |
front 2 DNA ligase: Joining pieces of DNA together Performing PCR reactions Digesting DNA Degrading protein | back 2 Joining pieces of DNA together |