1
Which of the following is NOT considered a significant advance in PCR methodology?
The invention of the thermal cycler
The use of reverse transcriptase to generate cDNA from single-stranded RNA
The use of agarose gel electrophoresis to visualize amplified products
The use of fluorescently-tagged probes for real-time quantitation of PCR
The use of agarose gel electrophoresis to visualize amplified products
2
DNA ligase:
Joining pieces of DNA together
Performing PCR reactions
Digesting DNA
Degrading protein
Joining pieces of DNA together