front 1 Those organisms that pose very little risk of disease for healthy students and they can still be capable of causing infection under certain circumstances are rated | back 1 BSL-1 |
front 2 Though we will not use these organisms during lab, these organisms can cause disease in healthy adults, pose a lethal risk, and do not respond to vaccines or antimicrobial therapies | back 2 BSL-4 |
front 3 Which container would you put a used coverslip in? | back 3 Broken glass container |
front 4 Which sections of the SDS contain general information about the chemical, identification hazards, compositions, safe handling practices, and emergency control measures | back 4 1-8 |
front 5 Which machine is used to heat a mixture and stir a mixture using electromagnetic forces? | back 5 Hot plate/magnetic stirrer |
front 6 Which piece of equipment measures the amount of material based on absorbance? | back 6 Spectrophotometer |
front 7 Which tool is used to move microorganisms between cultures and can be repeatedly sterilized? | back 7 Inoculating loop |
front 8 Which machine agitates a mixture in a test tube to make a homogenized mixture? | back 8 Vortex mixer |
front 9 Which piece of glassware is used to move liquid culture around an agar plate evenly? | back 9 Spreader |
front 10 Which pipet moves a fixed volume? | back 10 Pasteur piper |
front 11 Which lenses are typically binocular? | back 11 Ocular lens |
front 12 Which objective lens is used for quickly scanning the microscope slides? | back 12 4x |
front 13 This type of microscope causes the light source to hit the specimen at a severely oblique angle | back 13 Darkfield |
front 14 This is the bending of light as it passes from one medium to another | back 14 Refraction |
front 15 Which one of the following phylum is considered an algae? | back 15 Rhodophyta |
front 16 Which of the following is not in the group Alveolata? | back 16 Parabasilids |
front 17 Which one of the following bacterial phyla is Gram positive? | back 17 Firmicutes |
front 18 Which phylum contains the plant pathogenic fungi known as smuts and rusts? | back 18 Basidiomycota |
front 19 Which phylum is an animal microbe? | back 19 Platyhelminthes |
front 20 Which virus classification does not match the description? Class VI- double stranded DNA with reverse transcriptase | back 20 Class I- double-stranded DNA Class IV- single stranded RNA (+) |
front 21 Which type of stain does not stick to the cell, but dries around the cell boundary creating a silhouette? | back 21 Negative staining |
front 22 Which type of stain uses both basic and acidic dyes? | back 22 Positive staining |
front 23 Which type of stain uses two dyes- a primary dye and a counterstain? | back 23 Differential staining |
front 24 This type of differential stain reacts to a waxy material called mycolic acid in the bacterium's cell wall | back 24 Acid-fast staining |
front 25 The method completed in lab using crystal violet and safranin | back 25 Gram staining |
front 26 With Gram staining, Gram positive bacteria will stain as which color? | back 26 Purple/blue |
front 27 Which spore staining, vegetative cells will appear as which color? | back 27 Red |
front 28 With acid-fast staining method, non-acid-fast bacteria will stain which color? | back 28 Blue |
front 29 The first step in smear preparation from a broth is? | back 29 Draw a "target circle" on the bottom of the microscope slide |
front 30 Differential staining with an endospore stain _______ a heat-fixed smear | back 30 requires |
front 31 These media contain agar for thickening but do not form a firm substrate | back 31 Semisolid |
front 32 These media contain substances that absorb oxygen or slow the penetration of oxygen | back 32 Anaerobic growth |
front 33 This type of media contains one or more agents that inhibit the growth of certain microbes but not another | back 33 Selective |
front 34 This type of media has large molecules such as proteins, polysaccharides, and lipids that can vary greatly in exact composition | back 34 Complex media |
front 35 When agar media is placed in a test tube and is allowed to solidify vertically, it is called a | back 35 Deep |
front 36 This media is a general purpose media used for nonfastidious microbes including many bacteria and fungi | back 36 Nutrient Agar or both (NA or NB) |
front 37 This selective and differential media is used to isolate enteric bacteria that hydrolyze urea | back 37 EMB |
front 38 Which of the following is commonly used to culture algae? | back 38 Salt agar |
front 39 Which piece of glassware is used to sterilize media in? | back 39 Media bottle |
front 40 The method uses media to separate out individual microbes to make pure culture is known as | back 40 Isolation |
front 41 The first step when removing a colony from a slant is | back 41 Flame an inoculating loop |
front 42 The first step in inoculating an agar plate is | back 42 Open the lid of the petri dish |
front 43 Which tool is used to sterilize the inoculating loop? | back 43 Micro-incinerator |
front 44 Growing microbes in culture is important to increase visibility and manage microbes in an artificial environment is which of the following | back 44 Inoculation |
front 45 This method takes a mixed culture and selects a single colony to result in a pure culture | back 45 Sub-culturing |
front 46 This type of media can grow several types of microbes but are designed to bring out visible differences among those microbes | back 46 Differential |
front 47 Which selective media uses methylene blue to inhibit the growth of non-target bacteria? | back 47 EMB |
front 48 MSA is selective for which bacteria? | back 48 Staphylococcus sp. |
front 49 EMB agar is selective because only bacteria that ferments this sugar produce colored colonies | back 49 Lactose |
front 50 Colonies of E. coli on EMB agar will produce what color colonies? | back 50 Green |
front 51 In this phase, the cells reach the maximum rate of cell division | back 51 Log phase |
front 52 In this phase, there are few cells present | back 52 Lag phase |
front 53 How many cells are present after 20 generations (starting from 1 cell) of exponential growth? | back 53 1,048,576 |
front 54 A dilution of 1mL of bacteria from broth into 999mL broth or water | back 54 1:1000 |
front 55 A serial dilution of 1:10 followed by 1:10 gives a final dilution of | back 55 1:100 |
front 56 A serial dilution of 1:4 followed by 1:2 gives a final dilution of | back 56 1:8 |
front 57 A dilution of 0.1mL bacteria from broth in 999.9mL broth or water | back 57 1:10,000 |
front 58 A 1:1,000,000 is a common dilution factor, which serial dilution will end with this dilution? | back 58 10^-6 |
front 59 Which generation time is calculated from 30 x log 2 / (log 1257 - log 536)? | back 59 24.4 |
front 60 Two samples have the following absorbance readings 0.23 (A) and 0.17 (B). Which one has more cells present? | back 60 A |
front 61 The generation of cellular energy, ATP, can have metabolic reactions that require a particular amount of which physical property? | back 61 Oxygen levels |
front 62 Microbes that are flexible and can grow with or without oxygen are | back 62 Facultative anaerobe |
front 63 When using a fluid thioglycollate media which type of bacteria would be present at the top of the media? | back 63 Obligate aerobes |
front 64 Which bacteria have optimal growth between 45°C to 75°C? | back 64 Thermophiles |
front 65 When the temperature increases past the optimal, these denature | back 65 Enzymes |
front 66 When the temperature increases past the optimal, lipids can be destroyed affecting which structure? | back 66 Cell membrane |
front 67 When the temperature increases past the optimal, the hydrogen bonding in the RNA breaks down and the proteins denature | back 67 Ribosomes |
front 68 A bacterium that grows at a pH of 4 is a | back 68 Acidophile |
front 69 An environment that has more solutes than a cell is | back 69 Hypertonic |
front 70 These microbes can grow in moderate concentration of sodium chloride | back 70 Halotolerant |