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Week 9 final lab

front 1

Which type of organism requires oxygen as its final electron acceptor in the electron transport chain?

back 1

obligate aerobe

front 2

What might explain the reason why obligate aerobes cannot grow at all in the presence of oxygen

back 2

They lack enzymes to degrade toxic forms of oxygen

front 3

True or false obligate aerobes, obligate anaerobes aerotolerant anaerobes, and facultative anaerobes are all present at different places on in the human body

back 3

True

front 4

Where would you expect obligate anaerobes to grill at FTM culture tube

back 4

At the bottom

front 5

What type of environment is created in the gas pack jar

back 5

Anaerobic

front 6

Which of the following is likely classified for the organism in the FTM tube

back 6

Obligate aerobe

front 7

True or false the organism inoculated onto the plates was likely a facultative anaerobe

back 7

False

front 8

back 8

no data

front 9

back 9

no data

front 10

A bacterial species that cannot tolerate the presence of oxygen and will only grow in the absence of oxygen is classified as a(n)

back 10

obligate anaerobe

front 11

Some organisms exhibit flexibility in terms of their energy harvesting reactions. If provided with oxygen, they will obtain energy through aerobic respiration. If in an environment without oxygen, they will turn to fermentation to satisfy their energy needs. These organisms are classified as

back 11

Facultative anaerobes

front 12

Indicate which of the following are used for the cultivation of obligate anaerobes.

back 12

Anaerobic jar

CO2 incubator

front 13

What is the role of resazurin in fluid thioglycolate medium?

back 13

Resazurin indicates the presence of oxygen in the media

front 14

Inoculated tubes of fluid thioglycolate medium should be placed in an anaerobic jar during incubation.

back 14

Fasle

front 15

Where would you predict an obligate anaerobe would grow in a tube of fluid thioglycolate medium?

back 15

At the bottom of the tube

front 16

Where would you predict a facultative anaerobe would grow in a tube of fluid thioglycolate medium?

back 16

throughout the tube

front 17

The pink color in the top of the broth in an FTM tube indicates

back 17

the presence of oxygen

front 18

back 18

no data

front 19

Which parts of a cell are affected by temperature

back 19

DNA

Enzymes

RNA

Membrane fluidity

ribosomes

front 20

Both human pathogens and normal microbiota are typically classified as

back 20

mesophiles

front 21

You are studying a bacterium found in the normal intestinal flora of humans and most other mammals, and are doing an experiment to determine the optimum growth rate of this bacteria. You have inoculated five nutrient broths, and are incubating one broth at each of the following temperatures: 5°C, 25°C, 38°C, 42°C, and 55°C. At which temperature would you predict to see the optimum growth rate?

back 21

38 c

front 22

As temperature decreases, membrane fluidity increases

back 22

False

front 23

Listeria monocytogenes is a human pathogen, growing readily in the human body. It poses a problem in food storage because of its ability to grow in refrigerated foods, such as unpasteurized milk or deli meats. An organism that prefers moderate temperatures, such as 25–40°C but is capable of growth at refrigerator temperatures is known as a

back 23

psychrotoph

front 24

Taq polymerase is a DNA polymerase enzyme that demonstrates maximal activity at 72°C. The organism from which this enzyme was isolated would most likely be classified as a

back 24

thermophile

front 25

back 25

no data

front 26

back 26

no data