Microbiology: Micro Bio Chp 8 Exam 3 Flashcards

Recombinant DNA technology can be most accurately defined as the

In the 20th century, scientists harnessed the natural metabolic reactions of bacteria to make _____ for the first time in an industrial setting.

Which of the following items is NOT a part of the name of a restriction enzyme?

The natural role of restriction enzymes in bacteria is to

A library of cloned sequences representing the expressed genes of an organism is known as a

Which of the following restriction enzyme sites would produce blunt-ended fragments?

Recent modifications of microbes have produced

Which of the following statements regarding vectors is FALSE?

Synthetic nucleic acids are useful as

Mutagens are useful in biotechnology research for

Which of the following is essential to PCR?

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?

Which of the following would be an appropriate sequence of temperature for PCR?

If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?

In gel electrophoresis, DNA molecules move toward the _____ electrode because they have an overall _____ charge.

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?

A northern blot differs from a Southern blot in the

Which method of inserting DNA into cells is illustrated in this figure?

The DNA double helix can be separated into single strands using

The sequencing and analysis of an organism's genetic information is called

Which of the following devices is used for PCR?

DNA fingerprinting can be used

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?

Subunit vaccines are safer than traditional vaccines because they

Which of the following recombinant tools is NOT in DNA fingerprinting?

Transgenic organisms

Which of the following microbes produces a protein that kills a variety of insect pests?

Synthetic nucleic acids are produced

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

Which of the following procedures would be used to introduce DNA into a single mouse cell?

The procedure used to identify individuals by their unique genetic sequences is known as

An effective tool for screening a large number of genetic sequences at once is know as

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Two men may be the father of a child. this figure shows the results of a DNA fingerprint analysis to determine paternity. M is the mother, C is the child, A and B are the two men. Which is the best interpretation of these results?

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Synthesis of cDNA requires the use of

Probes used for detecting genetic sequence are frequently composed of

______ are used for cutting DNA molecules into fragments.

A microarray is assembled using

Which of the following is NOT a goal of recombinant DNA technology?

In Southern blotting, DNA molecules are immobilized on

_____ are used in the detection of DNA by FISH.

Injecting DNA into cells can be accomplished using

DNA encoding a normal gene is inserted into cells from a patient with a defective form of the gene. Recombinant cells are identified and isolated, and returned to the patients body. this is an example of?

Some of the strongest opposition to the application of recombinant DNA technology concerns

The use of microbes to make practical products such as vaccines or hormones is called (genomics/ recombination/ biotechnology)

A technique using fluorescent-tagged probes to detect specific DNA sequences in their natural locations is known as (FISH/ PCR/ BLOTTING)

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/victors/ clones).

Restriction (enzymes/ proteases), first isolated from bacterial cells, cut DNA molecules at specific sites.

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called (probes/ primers/ vectors)

A set of clones representing the entire genome of an organism is known as a gene (recombinant/ map/ library).

The northern blot is a technique used to detect specific RNA molecules in a larger population of molecules that have been separated by gel ( electroporation/ electrophoresis).

Small RNAs that bind to an mRNA and alter its expression are known as (antisense/ probes/ restriction) RNAs.

The process of locating genes within the nucleic acid of an organism is called (genetic/ library/ clone) mapping.

The insertion of foreign DNA directly into a cell's nucleus using a glass micro pipet is called (microporation/ electroporation/ microinjection).

Genetic (screening/ sequencing/ cloning) can be used to detect mutant genes associated with genetic diseases in individuals before any clinical symptoms are noted.

A DNA (library/ microarray) may be used to study the complex, changing patterns of mRNA production in an organism.

Beta-carotene, the biochemical precursor to vitamin A, can be added to rice by using (biotechnology/ cloning/ recombinant) DNA technology, thereby increasing its nutritional value.

Study of the genome of Deinococcus radiodurans may provide insight into preventing or correcting genetic damage resulting from (radiation/ mutation).

Vectors usually contain genetic (markers/sequences) such as antibiotic genes or fluorescent tags.