The Five I's of studying microorganisms include all of the following except
A. Inoculation
B. Incubation
C. Infection
D. Isolation
E. Identification

C

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium
is
A. Isolation
B. Inoculation
C. Immunization
D. Infection
E. Contamination

B

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium
is
A. Isolation
B. Inoculation
C. Immunization
D. Infection
E. Contamination

A

The correct microbiological term for the tiny sample of specimen that is put ino a nutrient medium in
order to produce a culture is the
A. Colony
B. Inoculum
C. Streak
D. Loop
E. None of the choices are correct

B

Which of the following is essential for into development of discrete, isolated colonies?
A. Broth medium
B. Differential medium
C. Selective medium
D. Solid medium
E. Assay medium

D

Which method often results in colonies developing down throughout the agar along with some colonies
on the surface?
A. Streak plate
B. Spread plate
C. Pour plate
D. All of the choices are correct
E. None of the choices are correct

C

What type of isolation technique is most effective for the majority of applications?
A. Pour plate
B. Streak plate
C. Spread plate
D. Loop dilution
E. Culture plate

B

Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?
A. A pure culture
B. A mixed culture
C. A solid medium
D. A liquid medium
E. A contaminated medium

C

Agar is an important component of media because
A. Bacteria require agar to grow
B. Agar inhibits mold growth
C. Agar provides a solid surface for bacterial growth
D. Agar prevents contamination
E. All of the choices are correct

C

The three physical forms of laboratory media are
A. Solid, liquid, gas
B. Solid, semisolid, liquid
C. Streak plate, pour plate, broth
D. Aerobic, anaerobic, micro aerobic
E. None of the choices are correct

B

Which of the following is not an inoculating tool?
A. Petri dish
B. Loop
C. Needle
D. Pipette
E. Swab

A

Agar is a complex polysaccharide that comes from a/an
A. Green plant
B. Fungus
C. Mold
D. Algae
E. Euglena

D

Which of the following is not a benefit of agar as a solid medium?
A. Flexibility
B. Holds moisture
C. Can be inoculated and poured at a temperature that is not harmful
D. Solid at room temperature
E. Is digested by most microbes

E

A nutrient medium that has all of its chemical components identified and their precise concentrations
known and reproducible, would be termed
A. Complex
B. Reducing
C. Enriched
D. Synthetic
E. None of the choices are correct

D

A nutrient medium that contains at least one ingredient that is NOT chemically definable would be
termed
A. Complex
B. Reducing
C. Enriched
D. Synthetic
E. None of the choices are correct

A

All of the following are examples of different types of microbiological media except
A. Broth
B. Enriched
C. Agar
D. Petri dish
E. Gel

D

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.
Following incubation, only the E. coli grows in the culture. What is the most likely explanation?
A. The microbiologist used too much inoculum
B. The culture is contaminated
C. The incubation temperature was incorrect
D. The culture medium must be selective
E. The culture medium must be differential

D

A common medium used for growing fastidious bacteria is
A. Blood agar
B. Trypticase soy agar
C. Mannitol salt agar
D. MacConkey medium
E. A reducing medium

A

A reducing medium contains
A. Sugars that can be fermented
B. Extra oxygen
C. Hemoglobin, vitamins or other growth factors
D. Substances that remove oxygen
E. Inhibiting agents

D

Which type of medium is able to distinguish different species or types of microorganisms based on an
observable change in the colonies or in the medium?
A. Differential
B. Selective
C. Enumeration
D. Enriched
E. Reducing

A

A microbiologist decides to use a nutrient medium that contains thioglycollic acid. What type of microbe
is she attempting to culture?
A. Fastidious
B. Gram positive
C. Anaerobic
D. Gram negative
E. Aerobic

C

Differential media results in which of the following growth characteristics?
A. Different color colonies
B. Different media color post incubation
C. Precipitates
D. Gas bubbles
E. All of the choices are correct

E

A reducing media is used to culture
A. Fastidious organisms
B. Aerobic organisms
C. Anaerobic organisms
D. Any pathogenic organisms
E. None of the choices are correc

C

For which bacterial genus is mannitol salt agar selective?
A. Salmonella
B. Streptococcus
C. Neisseria
D. Staphylococcus
E. Escherichia

D

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would
likely be present in selective media for analyzing this fecal specimen?
A. NaCl
B. Sheep red blood cells
C. Bile salts
D. Thioglycollic acid
E. Peptone

C

Bacteria that require special growth factors and complex nutrients are termed
A. Aerobic
B. Anaerobic
C. Fastidious
D. Microaerophilic
E. Autotrophic

C

A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both
Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.
What is the most likely explanation?
A. The microbiologist used too much inoculum
B. The culture is contaminated
C. The incubation temperature was incorrect
D. The culture medium must be selective
E. The culture medium must be differential

D

What is the term for a culture made from one isolated colony?
A. Axenic
B. Mixed
C. Pure
D. Both axenic and mixed
E. Both axenic and pure

E

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.
A. Streaked
B. Poured
C. Incubated
D. All of the choices are correct

C

The _____ of the microscope holds and allows selection of the objective lenses.
A. Stage
B. Condenser
C. Objective
D. Ocular
E. Nosepiece

E

which of the following will converge light rays to a single focal point on the specimen?
A. Ocular lens
B. Objective lens
C. Iris diaphragm
D. Condenser
E. Nosepiece

D

Which of the following magnifies the specimen to produce the real image of the specimen?
A. Condenser
B. Objective lens
C. Ocular lens
D. Body
E. Nosepiece

B

Which of the following magnifies the specimen to produce the virtual image of the specimen?
A. Objective lens
B. Ocular lens
C. Condenser
D. Body
E. Iris diaphragm

B

Which of the following controls the amount of light entering the specimen?
A. Objective lens
B. Ocular lens
C. Condenser
D. Body
E. Iris diaphragm

E

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power
of the objective lens if the ocular lens is 10X?
A. 100X
B. 950X
C. 85X
D. 850X
E. 95X

E

Magnification is achieved in a compound microscope through the initial magnification of the specimen
by the _____ lens. This image is then projected to the _____ lens that will further magnify the specimen
to form a virtual image received by the eye.
A. Ocular, objective
B. Scanning, objective
C. Objective, ocular
D. Ocular, oil
E. None of the choices are correct

C

Which of the following characteristics refers to the microscope's ability to show two separate entities as
separate and distinct?
A. Resolving power
B. Magnification
C. Refraction
D. All of the choices are correct
E. None of the choices are correct

A

All of the following are diameters of cells that would be resolved in a microscope with a limit of
resolution of 0.2µm except
A. 0.2 µm
B. 0.2 mm
C. 0.1 µm
D. 0.3 µm
E. 2.0 µm

C

The wavelength of light used and the numerical aperture governs
A. Illumination
B. Resolution
C. Magnification
D. Size of the field
E. All of the choices are correct

B

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the
slide to the objective lens.
A. The scanning objective
B. The oil objective
C. The slide
D. Immersion oil
E. The ocular

D

The type of microscope in which you would see brightly illuminated specimens against a black
background is
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron

B

Which type of microscope shows cells against a bright background and also shows intracellular structures
of unstained cells based on their varying densities?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Differential interference
E. Electron

C

Which type of microscope is the most widely used and shows cells against a bright background?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron

A

All of the following pertain to the fluorescence microscope except
A. Uses electrons to produce a specimen image
B. Type of compound microscope
C. Requires the use of dyes like acridine and fluoresce in
D. Commonly used to diagnose certain infections
E. Requires an ultraviolet radiation source

A

A confocal scanning optical microscope
A. Uses ultraviolet light to form a specimen image
B. Shows three-dimensional cell images from the cell surface to the middle of the cell
C. Produces specimen images on electron micrographs
D. Uses dyes that emit visible light when bombarded by ultraviolet rays
E. Requires specimens to be stained

B

A confocal scanning optical microscope
A. Uses a laser beam of light to form a specimen image
B. Shows only the surface of the specimen
C. Produces specimen images on electron micrographs
D. Uses dyes that emit visible light when bombarded by ultraviolet rays
E. Requires specimens to be stained

A

Which type of microscope does not use light in forming the specimen image?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron

E

Which type of microscope achieves the greatest resolution and highest magnification?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron

E

Which type of microscope cannot image live specimens?
A. Bright-field
B. Phase-contrast
C. Dark-field
D. Tunneling electron
E. Differential interference

D

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and
forth over it?
A. Fluorescence
B. Differential interference contrast
C. Scanning electron
D. Transmission electron
E. Phase-contrast

C

The specimen preparation that is best for viewing cell motility is
A. Hanging drop
B. Fixed stained smear
C. Gram stain
D. Negative stain
E. Flagellar stain

A

The purpose of staining cells on a microscope slide is to
A. Kill them
B. Secure them to the slide
C. Enlarge the cells
D. Add contrast in order to see them better
E. See motility

D

The gram staining procedure is best described as a __staining technique.
A. acid fast or Ziehl-Neelson
B. differential
C. capsule
D. Schaefer-Fulton

B

What do the Gram stain, acid-fast stain and endospore stain have in common?
A. Used on a wet mount of the specimen
B. Use heat to force the dye into cell structures
C. Outcome based on cell wall differences
D. Use a negative stain technique
E. Are differential stains

C

Basic dyes are
A. Attracted to the acidic substances of bacterial cells
B. Anionic
C. Used in negative staining
D. Repelled by cells
E. Dyes such as India ink and nigrosin

A

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.
All the cells appear blue under the oil lens. This is an example of
A. Negative staining
B. Using an acidic dye
C. Simple staining
D. Using the acid-fast stain
E. Capsule staining

C

Matching.
1.) 1 Km A.) 10-2 cm
2.) 1 mm B.) 100 mm
3.) 10 cm C.) 10-6 m
4.) 1 m D.) 1000 m

1/D, 2/A, 3/B, 4/C

Who invented the first crude microscope by grinding glass?
A. Redi
B. Lister
C. Schultz & Schwann
D. Leeuwenhoek

D

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be
tested from a satellite office site.
A. Transport
B. EMB
C. Blood
D. thioglycollate

A

The Gram staining procedure is best described as a __staining technique.
A. Acid fast or Ziehl-Neelson
B. Differential
C. Capsule
D. Schaefer-Fulton

B

The procedures for culturing a microorganism require the use of a microscope.
True False

False

A medium that is gel-like has less agar in it compared to a solid medium.
True False

True

Some microbes are not capable of growing on artificial media.
True False

True

A selective medium contains one or more substances that inhibit growth of certain microbes in order to
facilitate the growth of other microbes.
True False

True

One colony typically develops from the growth of several parent bacterial cells.
True False

False

Mixed cultures are also referred to as contaminated cultures.
True False

False

Bacterial cultures are easily identified from their microscopic appearance.
True False

False

Normal incubation temperatures range from 30° to 60° C.
True False

False

The bending of light rays as they pass from one medium to another is called refraction.
True False

True

The real image is the reverse of the actual specimen.
True False

False

A differential interference contrast microscope uses dyes to give colored three-dimensional images.
True False

False

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the
specimens.
True False

True

At the end of the Gram stain, gram positive bacteria will be seen as pink cells.
True False

False