Functional anatomy of prokaryotic and eukaryotic cells
Which of the following statement is incorrect regarding prokaryotic cells
they lack a plasma membrane
Each of the following statements concerning the gram-positive cell wall is true except
it protects the cell in a hypertonic environment
Which of the following statements best describes what happens when a bacterial cell is placed in a solution containing 5% NaCl
water will move out of the cell
A gram-positive bacterium suddenly acquires resistance to the antibiotic methicillin. this trait most likely occurred due to acquisition of new genetic information through
conjugation
by which of the following mechanisms can a cell transport a substance from a lower to a higher concentration
active transport
which of the following is not a typical characteristic of most bacterial plasma membranes
contains cholesterol
which one of the following organisms has a cell wall
fungi
which of the following statements is true
endospores allow a cell to survive environmental changes by producing a dormant period with no growth
which of the following pairs is mismatched
centrosomes - food storage
which of the following organelles most likely resembles a prokaryotic cell
mitochondrion
Which of the drawing is a tetrad
B
which drawing in figure 4.1 posseses an axial filament
A
which drawing in figure in 4.1 is streptococci
D
antibiotics that target cell wall synthesis ultimately cause bacterial cell death as a result of
osmotic lysis
bacteria are commonly used organism for studies of genetic material in the research laboratory. The nucleic acids must first be isolated from the cells for these studies. which of the following would most likely be used to lyse gram-positive bacterial cells for nucleic acid isolation
lysozyme
which of the following statements about gram-negative cell walls is false
they are sensitive to penicillin
which of the following structures is not found in some prokayotic cells
cilium
functions of the glycocalyx include all of the following except
binary fission
which acts like an invisibility cloak and protects bacteria from being phagocytized
capsule
which of the following is not part of the passive transport process
ATP
which of the following terms best describes the cell in figure 4.2
lophotrichous flagella
in bacteria, photosynthetic pigments are found in
chromatophores
the difference between simple diffusion and facilitated diffusion is that facilitated diffusion
requires transporter proteins
the term "run" and "tumble" are generally associated with
taxic movements of the cell in response to attractants or repellents
You have isolated a motile, gram-positive cell with no visible nucleus. you can safely assume that the cell
has a cell wall
Fimbriae and pili differ in that
pili are used to transfer DNA and motility
Which diagram of a cell wall is gram-negative cell wall
B
which diagram of a cell wall possesses lipid A/endotoxin responsible for symptoms associated with infection
B
which diagram of a cell wall has a structure that protects against osmotic lysis
both A and B
which diagram of a cell wall is decolorized by alcohol
B
which diagram of a cell wall is resistant to many antibiotics
B
which diagram of a cell wall contains teichoic acids
A
which diagram of a cell wall contains porins
A
where are phospholipids most likely found in a prokaryotic cell
the plasma membrane
where are phospholipids most likely found in a eukaryotic cell
the plasma membrane, around organelles, and surrounding flagella
which of the following is not found or observed to occur in both mitochondria and prokaryotes
cell wall
which of the following statements is correct about passive diffusion
it involves movement of molecules down a concentration gradient and may require a transport protein
oxygen crosses a plasma membrane
thorugh simple diffusion
in a hypertonic solution, a bacterial cell will typically
plasmolyze
what will happen if a bacteriacel is pretreated with a lysozyme solution, then placed in distilled water
the cell will undergo osmotic lysis
how do spirochetes and spirilla differ
spirilla have an external flagella but spirochetes have axial filaments
which one of the following pairs is mismatched
ribosomes - carbon storage
which of the following are not energy reseves
carboxysomes
Which of the following is not a functionally analogous pair
cilia - pilia
the DNA found in most bacterial cells
is circular in structure
the cell walls of bacteria are responsible for the shape of the bacteria and the difference in the gram stain reaction
true
antibiotics that target the cell wall are an effective treatment against many pathogenic bacteria
true
cells placed in a hypotonic solution tend to lose water due to osmotic pressure
false
small, hydrophobic molecules pass through a cell membrane most easily by diffusion
true
spheroplasts, protoplasts, and mycoplasms are bacterial cells without cell walls
true
endospores are a reproductive structure
false
the internal structure of eukaryotic cilia and flagella are the same
true
many enzymes in both prokaryotic and eukaryotic cells are compartmentalized within organelles
false
the number of organelles such as chloroplasts, mitochondria, and rough endoplasmic reticulum is the same in all eukaryotic cells
true
if you observe rod-shaped red cells after the gram stain, you can assume their cell walls contain endotoxin
true
characteristics of a typical prokaryotic and eukaryotic cell
prokaryotic cells' DNA is not enclosed within a membrane and is usually single.they usually lack organelles and their cell walls contain peptidoglycan. Eukaryotic cells' DNA is found in the cell's nucleus, they have a number os membrane-enclosed organelles and their cell walls are chemically simple
compare and contrast gram-positive and gram-negative cell walls
the application of alcohol dehydrates the peptidoglycan of gram positve to make it more impermeable to crystals violet iodine. in gram negative, alcohol dissolves the outer membrane and leaves small holes in the thin peptidoglycan layer through which crystal violet-iodine diffuse. Gram-positive cell walls consist of peptidoglycan while gram-negative contains a few layers of peptidoglycan and anouter membrane
What is a biofilm and what role does biofilm play in disease
a complex aggregation of microbes. microorganisms exists as single cells that float or swim independently on a liquid or attach to each other and some solid surface. it can cause infections and its bacteria are often resistant to antibiot
dipploccoci
remains in pairs after dividing
streptococci
divides and remain attached in chain-like patterns
spiral bacteria
vibrios - curved rods
spirillum - helical shape
spirochete - helical and flexible
the structure of a prokaryotic cell
capsule
fimbriae
chromosome
pilus
flagella
arrangements of bacterial flagella
gram positive bacterium
2 basal body
gram negative bacterium
4 basal bodies
gram positive cell wall
gram negative cell wall
prokaryotes
DNA is not enclosed withing a membrane, it is inside the cell
DNA is not associated with histones
Lack organelles
cell walls contain peptidoglycan
divide by binary fission
eukaryotes
DNA found in cell's nucleus
DNA consistently associated with histones and non histones
Have membrane -enclosed organelles
cell walls are chemically simple
cell division involves mitosis
arrangement of cells
cocci - round shape
bacilli -rod-like
spiral
monomorphic
maintains a single shape
pleomorphic
can have many shapes
glycocalyx
viscous, gelatinous sugar
extracellular polymeric substance
a glycocalyx that helps cells in a biofilm attach to their target environment and to each other
what is the difference between capsule and slime
capsule is neatly organized and firmly attached while slime is unorganized and loose
axial filament/endoflagella
bundles of fibrils that arise at the ends of the cell.
spirochete move by the means of
axial filaments
fimbriae
helps bacterias attach to the wall
can occur at the poles of the bacterial cell or can be evenly distributed over the entire surface of the cell
pili
longer than fimbriae and only one or two per cell. use to transfer plasmid
crystal violet
primary stain. Stains both gram positive/negative purple
Iodine
forms large crystals with the dye that are too large to escape through the cell wall
what happens to gram positive when alcohol is applied
it makes gram positive more impermeable to crystal violet-iodine
what happens to gram negative when alcohol is applied
alcohol dissolves the outermembrane and leaves holes for crystal violet to diffuse
what color does gram positive turn when safranin is applied
purple
what color does gram negative turn when safranin is applied
red
atypical cell walls
plasma membrane have sterols (lipids). protects them from lysis (rupture)
acid- fast cell walls
used to identify mycobacterium and norcardia
bacteria contain mycolic acid in their cell wall
can be stained with carbolfuchsin
protoplast
wall-less cell
spheroplast
remaining outer wall layer
Nucleoid
contains bacterial chromosome
the cell's genetic information which carries all the information required for the cell's structures and functions.
plasmid
double stranded DNA molecules. replicate independently of chromosomal DNA
ribosomes
protein synthesis take place
prokaryotic ribosome - 70s ribosomes
eukaryotic ribosome - 80s
50s, 30s