Columbia CNA with 5% sheep blood

is a differential, undefined, selective media that allows growth of gram positive organisms and stops or inhibits growth of most gram negative organisms

Which 3 bacteria does the Columbia CNA help grow?

staphylococci, streptococci, enteroccocci

What 6 carbon energy sources are in the Columbia CNA?

1. casein
2. digest of animal tissue
3. beef extract
4. yeast extract
5. corn starch
6. sheep blood

In Columbia CNA, sheep blood provides the _________ and yeast extract provides the ________

X factor
B vitamins

What attacks gram negative bacteria in the Columbia CNA and how?

antibiotics colistin and nalidixic acid (CNA) interfere with DNA replication and affect membrane integrity

Which three bacteria are CNA antibiotics effective against?

1. Klebsiella
2. Proteus
3. Pseudomonas

How does sheep's blood help with the differentiation of gram positive organisms?

based on hemolytic reaction