A Smear for the capsule requires heat-fixing.

False

The basic stain (Maneval's) is applied and remains for five minutes.

False

What is the purpose for the Congo red stain?

It stains the background.

What is the purpose of the Maneval's stain?

It colorizes the cell proper

Nigrosin can be used in place of Congo red.

True

This stain is both a structural and differential stain.

True

Identify 3 species of bacteria that may have capsules.

Bacillus anthracis, streptococcus pneumoniae, Klebsiella pneumoniae

The rinsing agent in the Schaeffer-Fultron Method of endospore staining is

DI water

The stain is both a structural and differential stain

True

The primary stain in the endospore stain is:

malachite green

What color are the vegetative cells in the endospore stain?

red

Heat is not necessary using the Schaeffer-Fulton method.

False

A hood is necessary using the Schaeffer-Fulton method

True

Identify 2 genera that have endospores.

Bacillus and Clostridium

Match the location of the endospore with the descriptive term.

Subterminal endospore- The endospore is between the middle and end of the cell.

Terminal endospore- The endospore is on the end of the cell.

Central endospore- The endospore is in the middle of the cell.

This stain is both a structural and differential stain

False

Match the flagella arrangement to the definition:

Amphitrichous- Flagella at both ends of the bacterium.

Peritrichous- Flagella covering the entire cell surface of the bacterium.

Monotrichous/polar- A single flagellum found on one end of the bacterium.

Lophotrichous- Tufts of flagella at one end of the bacterium.

Why can't flagella be observed in a living sample?

Bacterial flagella are too thin to be observed with the light microscope and ordinary stains. They need to be overstained and this kills them unfortunately.

Organisms with a capsule and endospores are easy to destroy.

False

All staining methods used in all the staining exercises performed in lab always color or stain the microbe.

False