Prelab for Spectrophotometric Determination of a Food-Dye SD
What food dye will you be testing for?
Red 40
What color does the food dye you will be testing for mostly absorb?
green
In the formula A = εbc what does the "A" represent?
absorbance
If the Alura Red food-dye stock solution has a molarity of 0.42 M and 2.00 mL of this stock solution is diluted to 100.00 mL, what is the concentration of the diluted solution?
Do not use scientific notation.
(0.42 M)(2.00 mL) = (C2)(100 mL)
C2 = 0.0084 M
Ex.
where C1 is the concentration (molarity) of the stock Allura Red solution, V1 is the amount of stock solution used, C2 is the concentration of the diluted solution, and V2 is the volume of the diluted solution.
An unknown food-dye is analyzed by the method used in this experiment. The absorbance of 5 standard solutions is plotted vs. their concentration (molarity) and a straight line is obtained with the formula:
Abs = 2.100x
An unknown food-dye solution is diluted and it's absorbance is found to be 0.65.
What is the molarity of the diluted unknown, to two significant figures?
Concentration (Molarity) = 0.65 / 2.100 = 0.31