Print Options

Card layout: ?

← Back to notecard set|Easy Notecards home page

Instructions for Side by Side Printing
  1. Print the notecards
  2. Fold each page in half along the solid vertical line
  3. Cut out the notecards by cutting along each horizontal dotted line
  4. Optional: Glue, tape or staple the ends of each notecard together
  1. Verify Front of pages is selected for Viewing and print the front of the notecards
  2. Select Back of pages for Viewing and print the back of the notecards
    NOTE: Since the back of the pages are printed in reverse order (last page is printed first), keep the pages in the same order as they were after Step 1. Also, be sure to feed the pages in the same direction as you did in Step 1.
  3. Cut out the notecards by cutting along each horizontal and vertical dotted line
To print: Ctrl+PPrint as a list

9 notecards = 3 pages (4 cards per page)

Viewing:

Exercise 27 Effects of Oxygen on Growth: Ignore TGYA, and Brewers agar

front 1

Tube inoculations

back 1

Tube inoculations

  1. coli : facultative anaerobe
  2. aeruginosa : obligate aerobe
  3. sporogenes : obligate anaerobe

front 2

Plate inoculations

back 2

Plate inoculations in air (incubator) vs anaerobe jar (gaspack)

  1. coli : facultative anaerobe
  2. aeruginosa : obligate aerobe
  3. sporogenes : obligate anaerobe

front 3

What is the role of oxygen in aerobic respiration?

back 3

Oxygen acts as a terminal electron acceptor during respiration.

front 4

What type(s) of metabolism can occur in the absence of oxygen?

back 4

In the absence of oxygen, anaerobic respiration or fermentation occurs.

front 5

Name two enzymes that are present in obligate aerobes but lacking in obligate anaerobes. What is the function of each enzyme?

back 5

Obligate anaerobes lack superoxide dismutase, which converts toxic superoxide to a harmless compound, and catalase, which converts toxic peroxide to oxygen.

front 6

Differentiate between a microaerophile and an aerotolerant organism

back 6

Microaerophiles require low amounts of oxygen for metabolism, although higher concentrations are lethal. Aerotolerant organisms can grow in oxygen but do not use it for metabolism and are thus indifferent to its presence.

front 7

Why is resazurin a useful media additive for the study of anaerobes?

back 7

Resazurin is an indicator for the presence of oxygen

front 8

Why is a GasPak anaerobic jar necessary for the cultrue of anaerobes on Brewer's anaerobic agar plates but not in tubes of fluid thioglycollate medium (FTM)?

back 8

Change the question to regular TSA instead of Brewer’s Agar: The GasPak anaerobic jar is necessary to remove the oxygen for anaerobic growth. Anaerobes grow in fluid thioglycollate medium because in the depths of the tube, the conditions are anaerobic; therefore the GasPak is unnecessary.

front 9

Where in the human body would you expect to find an obligate aerobe? Where would you expect to find an obligate anaerobe? Look up some examples of each and indicate where they may be found in the body?

back 9

Aerobes can be found on the human skin, e.g. Staphylococcus. Anaerobes can be found in hair follicles, e.g., Propionibacterium acnes and in the intestinal tract, e.g., Bacteroides fragilis. (knowing names of these species is not important)