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Micro Chapter 3
front 1 2) What structure does light pass through after leaving the condenser in a compound light microscope? A) ocular lens B) objective lens C) specimen D) illuminator | back 1 C) specimen |
front 2 3) Which of the following pairs is mismatched? A) nigrosin - negative stain B) methylene blue - simple stain C) acidic dye - capsule stain D) basic dye - negative stain E) crystal violet - simple stain | back 2 D) basic dye - negative stain |
front 3 4) Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet 3-Safranin 4-Iodine A) 1-2-3-4 B) 2-1-4-3 C) 2-4-1-3 D) 4-3-2-1 E) 1-3-2-4 | back 3 C) 2-4-1-3 |
front 4 5) Which of the following pairs is mismatched? A) alcohol-acetone — decolorizer B) crystal violet — basic dye C) safranin — acid dye D) iodine — mordant E) carbolfuchsin — basic dye | back 4 C) safranin — acid dye |
front 5 6) Which of the following is NOT true regarding the acid-fast stain? A) It is used to identify members of the genus Mycobacterium. B) Acid-fast cells retain the primary dye after treatment with acid-alcohol. C) If cells are acid-fast, they are gram-negative. D) Acid-fast cells appear red in a completed acid-fast stain. E) Non-acid-fast microbes appear blue in a completed acid-fast stain. | back 5 C) If cells are acid-fast, they are gram-negative. |
front 6 7) The purpose of a mordant in the Gram stain is to A) remove the simple stain. B) make the bacterial cells larger. C) make the flagella visible. D) prevent the crystal violet from leaving the cells. E) make gram-negative cells visible. | back 6 D) prevent the crystal violet from leaving the cells. |
front 7 8) Which of the following places the steps in the correct sequence? 1-Staining 2-Making a smear 3-Fixing A) 1-2-3 B) 3-2-1 C) 2-3-1 D) 1-3-2 E) The order is unimportant. | back 7 C) 2-3-1 |
front 8 9) The negative stain is used to A) visualize endospores. B) determine Gram reaction. C) determine flagella arrangement. D) visualize capsules. E) determine cell size. | back 8 E) determine cell size. |
front 9 10) Simple staining is often necessary to improve contrast in which microscope? A) compound light microscope B) phase-contrast microscope C) darkfield microscope D) fluorescence microscope E) electron microscope | back 9 A) compound light microscope |
front 10 11) Which microscope is used to see internal structures of cells in a natural state? A) compound light microscope B) phase-contrast microscope C) darkfield microscope D) fluorescence microscope E) electron microscope | back 10 B) phase-contrast microscope |
front 11 12) Which microscope uses visible light? A) confocal microscope B) differential interference contrast microscope C) fluorescence microscope D) scanning acoustic microscope E) scanning electron microscope | back 11 B) differential interference contrast microscope |
front 12 13) Which microscope achieves the highest magnification and greatest resolution? A) compound light microscope B) phase-contrast microscope C) darkfield microscope D) fluorescence microscope E) electron microscope | back 12 E) electron microscope |
front 13 14) In using this microscope, the observer does NOT look directly at an image through a lens. A) compound light microscope B) phase-contrast microscope C) darkfield microscope D) fluorescence microscope E) electron microscope | back 13 E) electron microscope |
front 14 16) A virus measures 100 nm in length. What is its length in μm? A) 10 μm B) 1 μm C) 0.1 μm D) 0.01 μm E) 0.001 μm | back 14 C) 0.1 μm |
front 15 18) A microorganism measures 5 μm in length. Its length in mm would be A) 500 mm. B) 50 mm. C) 0.5 mm. D) 0.05 mm. E) 0.005 mm. | back 15 E) 0.005 mm. |
front 16 19) Which of the following correctly traces the path of light through the compound microscope? A) light source; condenser; specimen; objective lens; ocular lens B) condenser; light source; specimen; ocular lens; objective lens C) light source; specimen; condenser; objective lens; ocular lens D) condenser; light source; specimen; objective lens; ocular lens E) light source; condenser; objective lens; specimen; ocular lens | back 16 A) light source; condenser; specimen; objective lens; ocular lens |
front 17  In Figure 3.1, line "c" points to the microscope's A) illuminator. B) condenser. C) ocular lens. D) objective lens. | back 17 B) condenser. |
front 18 23) Which microscope is most useful for visualizing a biofilm? A) compound light microscope B) phase-contrast microscope C) fluorescence microscope D) scanning acoustic microscope E) transmission electron microscope | back 18 D) scanning acoustic microscope |
front 19 25) You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point? A) purple B) red C) colorless D) brown | back 19 A) purple |
front 20 26) You are performing a Gram stain on gram-negative bacteria and you stop after the addition of the mordant. What is the appearance of the bacteria at this point? A) purple B) red C) colorless D) brown | back 20 A) purple |
front 21 27) You are performing a Gram stain on gram-negative bacteria and you stop after the decolorizer step. What is the appearance of the bacteria at this point? A) purple B) red C) colorless D) brown | back 21 C) colorless |
front 22 28) You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the counterstain. What is the appearance of the bacteria at this point? A) purple B) red C) colorless D) brown | back 22 A) purple |
front 23 29) Which type of stain is most useful in helping clinicians to decide which antibiotic to prescribe for a bacterial infection? A) negative stain B) simple stain C) Gram stain D) endospore stain E) flagella stain | back 23 C) Gram stain |
front 24 31) What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens? A) 4.5x B) 10x C) 45x D) 100x E) 450x | back 24 E) 450x |
front 25 36) Which microscope is used to see detail of a 300-nm virus? | back 25 E) electron microscope |
front 26 38) Which step in the Gram stain is the critical step in differentiating gram-positive cells from gram-negative cells? A) safranin B) alcohol-acetone C) iodine D) crystal violet | back 26 B) alcohol-acetone |
front 27 40) Which microscope is best used for observing the surfaces of intact cells and viruses? A) phase-contrast microscope B) darkfield microscope C) fluorescence microscope D) brightfield microscope E) scanning electron microscope | back 27 E) scanning electron microscope |
front 28 41) Bacterial smears are fixed before staining to A) affix the cells to the slide. B) make their walls permeable. C) accept stain. D) make the cells visible. | back 28 A) affix the cells to the slide. |
front 29 43) A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put immersion oil on the slide. The specimen will appear
| back 29
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front 30 44) The purpose of the ocular lens is to A) improve resolution. B) magnify the image from the objective lens. C) decrease the refractive index. D) increase the light. E) decrease the light. | back 30 B) magnify the image from the objective lens. |
front 31 45) In microscopy, the term resolution A) refers to the ability to distinguish fine structure and detail in a specimen. B) is the same as the total magnification of a specimen. C) is improved when longer wavelengths of light are employed. D) is only observed in stained specimens. E) refers to magnification when using the electron microscope. | back 31 A) refers to the ability to distinguish fine structure and detail in a specimen. |