front 1 Defined media | back 1 . ingredients are exact and known; Undefined – the ingredients are known but not the exact quantities |
front 2 All-purpose medium | back 2 used to increase the number of species grown |
front 3 Differential medium | back 3 has an indicator to inform you if a product was made or digested (pH indicator) |
front 4 Explain why there is no universal mediumcapable of growing all bacteria. | back 4 Microbes frequently require very specific nutrients and enviornments in which they thrive and grow. |
front 5 Name five all-purpose media. | back 5 1. TSA |
front 6 Alpha hemolysis | back 6 greenish/browning of blood agar; partial destruction of RBCs |
front 7 Beta hemolysis | back 7 clearing of blood agar; complete destruction of RBCs and hemoglobin |
front 8 Gamma hemolysis | back 8 no change in blood agar, bacteria is simply growing; nonhemolysis |
front 9 Understand why,when streaking a blood agar plate, the medium was stabbed with the loop upon completion of the streaking. | back 9 Hemolysins produced by streptococci ( streptolysins ) express optimally in anaerobic conditions, so the stabs encourage streptolysin activity because of the reduced oxygen concentration of the subsurface environment. |
front 10 Assuming that all of the organisms cultivated in this exercise came from the throats of healthy students, why is it important to cover and tape the plates? | back 10 prevents contamination of plate and also because microbes are opportunistic and not always pathogenic |
front 11 Why is the streak plate preferred over the spot inoculations in this procedure? | back 11 Quadrant streak is better for colony isolation as you start out with a much denser sample and then isolate it down , making the test more reliable |
front 12 Pseudomonas Agar F | back 12 enhances bacterial generation of fluorescein (fluorescent pigment that glows yellow-green) and inhibits formation of pyocyanin (a blue-green nonfluorescent pigment) |
front 13 Pseudomonas Agar P | back 13 enhances bacterial formation of pyocyanin (blue-green pigment) and inhibits generation of fluorescein (fluorescent pigment, glows yellow-green) |
front 14 Describe the value of exoenzymes to bacteria | back 14 Exoenzymes can breakdown macromolecules (i.e. starch) into smaller molecules that can be taken through the cell's membrane |
front 15 Suppose you had poured iodine on your plate and noticed clearing in the inoculated area, as well as around both of your transferred cultures.
| back 15 Starch was not mixed properly, clearings suggest contamination not seen before iodine was added |
front 16 How would you expect both positive and negative results to be affected if you were to add glucose to the medium? | back 16 The bacteria expected to eat starch will first use glucose since it ’ s easier to use as a substrate, so we won't be able to test for starch depletion using Iodine anymore because positive colonies may no longer test positive |
front 17 Exoenzyme | back 17 Digestive enzyme excreted from cells to then be phagocytized for nutrient absorption within the cell |
front 18 Endoenzyme | back 18 Non-digestive enzyme that functions within the cell. Most enzymes fall under this category |
front 19 Hydrolyze | back 19 add water to split molecular bond |
front 20 Understand the selective and differential aspects of MacConkey Agar | back 20 .. |
front 21 How is MacConkey Agar modified to allow the study of Enterococcus spp. and Staphylococcus spp | back 21 ... |
front 22 What is the complete definition of a coliform? | back 22 ______ are a group of bacteria that are used as indicators of the potential presence of pathogens, viruses or parasites in a sample. These microbes are defined as a group of bacteria having specific properties including the ability to grow at 35C in the presence of bile salts and able to ferment lactose. |
front 23 What defines the family Enterobacteriaceae? | back 23 a family of gram-negative, facultatively anaerobic, rod-shaped bacteria, usually motile, made up of saprophytes and plant and animal parasites of worldwide distribution, found in soil, water, and plants and in animals from insects to humans. have been responsible for as many as half of the nosocomial infections reported annually in the United States, most frequently by species of Escherichia, Klebsiella, Enterobacter,Proteus, Providencia, and Serratia. |
front 24 What seperates a coliform from enterobacteriaceae? | back 24 Coliforms are able to ferment lactose |