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136 notecards = 34 pages (4 cards per page)

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Microbiology Lab Practical

front 1

Condenser

back 1

Focuses light through specimen

front 2

Iris Diaphragm

back 2

Delivers concentrated beam of light to specimen

front 3

Coarse Adjustment Knob

back 3

For focusing on scanning. When the knob is turned, the stage moves up or down in order to coarse adjust the focus.

front 4

Fine Adjustment Knob

back 4

For precise focusing once initial focusing has been done.

front 5

Illuminator

back 5

Light Source

front 6

Stage

back 6

Holds microscope slide in position

front 7

Ocular Lens

back 7

Remagnifies image framed by objective lens.

front 8

Objective Lens

back 8

4 objective lenses on a microscope, consisting of 4X, 10x, 40x, nd 100x magnification powers. In order to obtain total magnification of an image, multiply eyepiece lens power by objective lens power.

front 9

Labeled Microscope

back 9

front 10

If you're looking at something under the microscope and want to move the slide backwards, what would you do?

back 10

Move it forward,

front 11

How to prepare an organism for staining

back 11

  1. Smear
  2. Air dry
  3. Heat fix

front 12

What's a negative stain?

back 12

When you stain the background and the organism is clear.

front 13

When do you do a negative stain?

back 13

When you can't heat fix an organism.

front 14

What color is a gram positive organism?

back 14

Purple

front 15

What color s a gram negative organism?

back 15

Red

front 16

A simple stain is...

back 16

Just coloring it.

front 17

Gram Stain Steps

back 17

  1. Crystal Violet
  2. Iodine
  3. Alcohol
  4. Safranin

front 18

If an acid fast stain is positive it is positive for...

back 18

Mycobacterium

front 19

If you do an endospore stain and find endospores, it is positive for what 2 genuses?

back 19

Bacillus and Clostridum.

front 20

Endospore Stain Procedure

back 20

  • Malachite Green
  • Wash with water
  • Safranin

(red rods, green cocci)

front 21

Acid Fast Stain

back 21

front 22

Acid fast Pos/Neg

back 22

front 23

Capsule Stain

back 23

front 24

Endospore Stain

back 24

front 25

Blood Agar is selective for...

back 25

Streptococcus

front 26

Blood Agar is differential for...

back 26

An organism's ability to hemolyze red blood cells

front 27

Eosin Methylene Blue (EMB) Agar is selective for...

back 27

Members of enteric bacteria (Gram negative rods)

front 28

Eosin Methylene Blue (EMB) Agar is differential for...

back 28

An organism's ability to ferment lactose.

front 29

Color of nonpathogen on EMB Agar?

back 29

Green

front 30

Color of weak pathogen on EMB Agar?

back 30

Pink

front 31

Color of strong pathogen on EMB Agar?

back 31

White

front 32

MacConkeys Agar is selective for....

back 32

Enteric gut bacteria

front 33

MacConkeys Agar is differential for...

back 33

An organism's ability to ferment lactose

front 34

Color of nonpathogen on MAC Agar?

back 34

Pink

front 35

Color of pathogen on MAC Agar?

back 35

White

front 36

Mannitol Salt Agar is selective for...

back 36

Staphylococcus aureus

front 37

Mannitol Salt Agar is differential for...

back 37

An organism's ability to ferment the carbohydrate mannitol

front 38

Color of a pathogen on Mannitol Salt Agar plate?

back 38

Yellow

front 39

Color of nonpathogen on Mannitol Salt Agar plate?

back 39

Red

front 40

Blood Agar Plate Hemolysis

back 40

front 41

Blood Agar Plate Hemolysis 2

back 41

front 42

MSA Pos/Neg Colors

back 42

front 43

MSA organisms that are pos/neg

back 43

front 44

EMB pos/neg

back 44

front 45

EMB organisms that are pos/neg

back 45

front 46

MAC pos/neg

back 46

front 47

MAC organisms that are pos/neg

back 47

front 48

What does a positive Oxidase test look like?

back 48

Dark blue/purple

front 49

What does a negative Oxidase test look like?

back 49

No color change

front 50

What is the color indicator in a fermentation test/MSA plate?

back 50

Phenol Red

front 51

What does a positive Catalase test look like?

back 51

Bubbles

front 52

What does a negative Catalase test look like?

back 52

No bubbles

front 53

What does a positive Nitrate Reduction test look like?

back 53

Gas (non fermenter), Red color (after addition of reagents A and B), No color change (after addition of Zinc dust.)

front 54

What does a negative Nitrate Reduction test look like?

back 54

No gas, Red color (after addition of Zinc dust.)

front 55

What gas is produced from a fermentation test?

back 55

Carbon Dioxide

front 56

What does a positive Phenol Red test look like?

back 56

Yellow

front 57

What does a negative Phenol red test look like?

back 57

Red

front 58

What does a positive Methyl Red test look like?

back 58

Red

front 59

What does a negative Methyl Red test look like?

back 59

No color change; yellow

front 60

What does a positive Voges-Proskauer test look like?

back 60

Red ring; red

front 61

What does a negative Voges-Proskauer test look like?

back 61

No color change; yellow

front 62

What does a positive Starch Hydrolysis test look like?

back 62

Halo

front 63

What does a negative Starch Hydrolysis test look like?

back 63

No halo

front 64

What does a positive Citrate test look like?

back 64

Blue, or there is no color change but growth is present

front 65

What does a negative Citrate test look like?

back 65

Green, no growth

front 66

What does a positive Sulfur Reduction test look like?

back 66

Black

front 67

What does a negative Sulfur Reduction test look like?

back 67

No black in medium; yellow

front 68

What does a positive Indole test look like?

back 68

Red ring

front 69

What does a negative Indole test look like?

back 69

No color change; yellow

front 70

What does a positive Motility test look like?

back 70

Growth radiating outwards from stab line

front 71

What does a negative Motility test look like?

back 71

No radiating growth

front 72

What does a positive Gelatinase test look like?

back 72

Liquid

front 73

What does a negative Gelatinase test look like?

back 73

Solid

front 74

What does a positive Urease test look like?

back 74

Fuchsia (pink)

front 75

What does a negative Urease test look like?

back 75

Yellow

front 76

Oxidase test

back 76

front 77

Catalase test

back 77

front 78

Nitrate Reduction test

back 78

front 79

Methyl Red test

back 79

front 80

Voges-Proskauer test

back 80

front 81

Phenol Red Fermentation test

back 81

front 82

Starch Hydrolysis test

back 82

front 83

Citrate test

back 83

front 84

Sulfur test

back 84

front 85

Indole test

back 85

front 86

Motility test

back 86

front 87

Geltinase test

back 87

front 88

Urease test

back 88

front 89

back 89

  • Fungi
  • Yeast
  • Saccharomyces cerevisiae

front 90

back 90

  • Fungi
  • Sporagiospore

front 91

back 91

  • Fungi
  • Zygospore

front 92

back 92

  • Fungi
  • Conidiospore
  • Penicillum

front 93

back 93

  • Fungi
  • Apothecium
  • Pezzia cup

front 94

back 94

  • Fungi
  • Corpinus sp.
  • Mushroom

front 95

back 95

  • Lichen

front 96

back 96

  • Protozoa
  • Amoeba

front 97

back 97

  • Protozoa
  • Paramecium

front 98

back 98

  • Protzoa
  • Euglena

front 99

back 99

  • Protozoa
  • Trichomas

front 100

back 100

  • Protozoa
  • Trypanosoma

front 101

back 101

  • Protozoa
  • Plasmodium

front 102

back 102

  • Trematodes
  • Asian Liver Fluke

front 103

back 103

  • Cestodes tapeworm
  • Scolex

front 104

back 104

  • Cestodes tapeworm
  • Proglottids

front 105

back 105

  • Nematodes
  • Enterobius vermicularis Adult

front 106

back 106

  • Nematodes
  • Necator Americanus larvae

front 107

UV light works by Inducting genetic mutations called...

back 107

Thymine Dimmers

front 108

What kind of Agar does the Kirby-Bauer test use?

back 108

Mueller-Hinton

front 109

What do you measure in a Kirby-Bauer test?

back 109

Zone of inhibition

front 110

What do we measure the Zone of Inhibition in?

back 110

Millimeters

front 111

What does the Membrane Filter Technique use?

back 111

Eosin Methylene Blue agar (EMB) in combination with a membrane filter

front 112

When is water considered potable in the Membrane Filter Technique?

back 112

If the count is less than 1 coliform per 100 mLs.

front 113

What color is Methylene blue when it is oxidized?

back 113

Blue

front 114

What color is Methylene blue when it is reduced?

back 114

Colorless

front 115

What is an indicator of good quality milk in a Methylene Blue Reductase test?

back 115

It takes more than 6 hours to turn from blue to white.

front 116

What is the pH indicator in the Snyder test?

back 116

Bromcresol blue

front 117

What does a positive Snyder test look like?

back 117

Yellow

front 118

What does a negative Snyder test look like?

back 118

Green

front 119

The Snyder test is a test to detect...

back 119

The prescience of Lactobacillus in saliva as an indicator of dental care susceptbility.

front 120

Methylene Blue test

back 120

front 121

Snyder test

back 121

Positive is yellow

front 122

What does color change in less than 24 hours mean on a Snyder test?

back 122

High susceptibility

front 123

What does color change in less than 48 hours mean on a Snyder test?

back 123

Moderate susceptibility

front 124

What does no color change 72 hours mean on a Snyder Test?

back 124

Negative result or low susceptibility.

front 125

The Oxidase test differential for

back 125

Enterobacteriales and Pseudomonas

front 126

Phenol red differentiates between ______________ and _____________

back 126

E.Coli and Proteus vulgaris

front 127

MR/VP differential for

back 127

E.Coli and Enterobacter aerogenes

front 128

Catalase test differential for

back 128

Staphylococcus areus and Streptococcus

front 129

Nitrate Reduction differentiates for

back 129

Pseudomonas, Bacillus subtitles, e. coli

front 130

Citrate test differential for

back 130

E.Coli and Enterobacter aerogenes

front 131

Starch Hydrolysis differential for

back 131

Bacillus subtitles, pseudomonas arguenosa, e.coli

front 132

Urease differential for

back 132

Proteus and Enterobacter

front 133

Gelatinase differential for

back 133

E.Coli and Proteus

front 134

Sulfur differential for

back 134

E.Coli, Salmonella, Shigella

front 135

Indole differential for

back 135

E.Coli, Salmonella, Shigella

front 136

Motility differential for

back 136

E.Coli, Salmonella, Shigella