front 1 | back 1 Serratia Marcescens |
front 2 | back 2 Micrococcus luteus |
front 3 | back 3 Ameba proteus |
front 4 | back 4 Plasmodium falciparum |
front 5 | back 5 Staphylococcus epidermidis |
front 6 | back 6 Bacillus megaterium |
front 7 | back 7 E Coli, Pseudomonas, and Myobacterium |
front 8 | back 8 Trypanosoma cruzi |
front 9 | back 9 Trichomonas Vaginalis |
front 10 | back 10 Paramecium |
front 11 | back 11 Bacillus megaterium |
front 12 Steps of Gram Stain | back 12 1. Crystal Violet (Primary) 1 min 2. Iodine (Mordant) 1 min 3. Alcohol (Decolorizer) 5-10 secs 4. Safranin (Counterstain) 1 min |
front 13 Acid Fast Stain | back 13 1. Carbolfuchsin (Primary) USE Bibulous Paper! 2. Heat (Mordant) 5-10 mins 3. Acid Alcohol (Decolorizer) 5-10 secs 4. Methylene Blue (Counterstain) 1 min |
front 14 Endospore Stain | back 14 1. Malachite Green (Primary) USE Bibulous Paper! 2. Heat (Mordant) 5-7 mins 3. Rinse with Water (Decolorizer) 4. Safranin (Counterstain) 1 min |
front 15 Gram Stain Positive Color and Why | back 15 Purple, Thick Peptidoglycan |
front 16 Gram Stain Negative Color and Why | back 16 Red, Thin Peptidoglycan |
front 17 Acid Fast Stain Positive Color and Why | back 17 Red, Contains Mycolic Cell Wall |
front 18 Acid Fast Stain Negative Color and Why | back 18 Blue, Lacks Mycolic Cell Wall |
front 19 Reason for Endospore Stain | back 19 To distinguish between metabolically active vegetative cells and dormant, resistant spores |