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Chapter 5

front 1

1) Which of the following compounds is NOT an enzyme?

A) dehydrogenase

B) cellulase

C) coenzyme A

D) β-galactosidase

E) sucrase

back 1

Answer: C

front 2

2) Figure 5.1

Which compound is being reduced in the reaction shown in Figure 5.1?

A) isocitric acid and α-ketoglutaric acid

B) α-ketoglutaric acid and NAD+

C) NAD+

D) NADH

E) NADH and isocitric acid

back 2

Answer: C

front 3

3) Which organism is NOT correctly matched to its energy source?

A) photoheterotroph - light

B) photoautotroph - CO2

C) chemoautotroph - Fe2+

D) chemoheterotroph - glucose

E) chemoautotroph-NH3

back 3

Answer: B

front 4

4) Which of the following statements about anaerobic respiration is FALSE?

A) It yields lower amounts of ATP when compared to aerobic respiration.

B) The complete Kreb's cycle is utilized.

C) It involves the reduction of an organic final electron acceptor.

D) It generates ATP.

E) It requires cytochromes.

back 4

Answer: C

front 5

What type of reaction is in Figure 5.2?

A) decarboxylation

B) transamination

C) dehydrogenation

D) oxidation

E) reduction

back 5

Answer: B

front 6

6) What is the fate of pyruvic acid in an organism that uses aerobic respiration?

A) It is reduced to lactic acid.

B) It reacts with oxaloacetate to form citrate.

C) It is oxidized in the electron transport chain.

D) It is catabolized in glycolysis.

E) It is converted into acetyl CoA.

back 6

Answer: E

front 7

How would a noncompetitive inhibitor interfere with a reaction involving the enzyme shown in Figure 5.3?

A) It would bind to a.

B) It would bind to b.

C) It would bind to c.

D) It would bind to d.

E) The answer cannot be determined based on the information provided.

back 7

Answer: B

front 8

How is ATP generated in the reaction shown in Figure 5.4?

A) glycolysis

B) fermentation

C) photophosphorylation

D) oxidative phosphorylation

E) substrate-level phosphorylation

back 8

Answer: E

front 9

9) Fatty acids are oxidized in

A) the Krebs cycle.

B) the electron transport chain.

C) glycolysis.

D) the pentose phosphate pathway.

E) the Entner-Doudoroff pathway.

back 9

Answer: A

front 10

Which of the graphs in Figure 5.5 best illustrates the activity of an enzyme that is saturated with substrate?

A) a

B) b

C) c

D) d

E) e

back 10

Answer: C

front 11

11) Which of the following is the best definition of oxidative phosphorylation?

A) Electrons are passed through a series of carriers to O2.

B) A proton gradient allows hydrogen ions to flow back into the cells through transmembrane protein channels, releasing energy that is used to generate ATP.

C) ATP is directly transferred from a substrate to ADP.

D) Electrons are passed through a series of carriers to an organic compound.

back 11

Answer: B

front 12

12) Which of the following statements about substrate-level phosphorylation is FALSE?

A) It involves the direct transfer of a high-energy phosphate group from an intermediate metabolic compound to ADP.

B) No final electron acceptor is required.

C) It occurs in glycolysis.

D) The oxidation of intermediate metabolic compounds releases energy that is used to generate ATP.

E) It occurs to a lesser degree in the Krebs cycle than in glycolysis.

back 12

Answer: D

front 13

13) Which of the following statements about photophosphorylation is FALSE?

A) Light liberates an electron from chlorophyll.

B) The oxidation of carrier molecules releases energy.

C) Energy from oxidation reactions is used to generate ATP from ADP.

D) It requires CO2.

E) It occurs in photosynthesizing cells.

back 13

Answer: D

front 14

14) A strictly fermentative bacterium produces energy

A) by glycolysis only.

B) by aerobic respiration only.

C) by fermentation or aerobic respiration.

D) only in the absence of oxygen.

E) only in the presence of oxygen.

back 14

Answer: A

front 15

15) The advantage of the pentose phosphate pathway is that it produces all of the following EXCEPT

A) precursors for nucleic acids.

B) precursors for the synthesis of glucose.

C) three ATPs.

D) NADPH.

E) precursors for the synthesis of amino acids.

back 15

Answer: C

front 16

16) Which biochemical process is NOT used during glycolysis?

A) substrate-level phosphorylation

B) oxidation-reduction

C) carbohydrate catabolism

D) beta oxidation

E) enzymatic reactions

back 16

Answer: D

front 17

17) In noncyclic photophosphorylation, O2 is released from

A) CO2.

B) H2

C) C6H12O6.

D) sunlight.

E) chlorophyll.

back 17

Answer: B

front 18

18) Which of the following is the best definition of fermentation?

A) the partial reduction of glucose to pyruvic acid

B) the partial oxidation of glucose with organic molecules serving as electron acceptors

C) the complete catabolism of glucose to CO2and H2O

D) the production of energy by oxidative-level phosphorylation

E) the production of energy by both substrate and oxidative phosphorylation

back 18

Answer: B

front 19

19) Which of the following is NOT necessary for respiration?

A) cytochromes

B) flavoproteins

C) a source of electrons

D) oxygen

E) quinones

back 19

Answer: D

front 20

20) Which one of the following would you predict is an allosteric inhibitor of the Krebs cycle enzyme, α-ketoglutarate dehydrogenase?

A) citric acid

B) α-ketoglutaric acid

C) NAD+

D) NADH

E) ADP

back 20

Answer: D

front 21

21) In green and purple bacteria, electrons to reduce CO2 can come from

A) CO2.

B) H2

C) C6H12O6.

D) H2

E) chlorophyll.

back 21

Answer: D

front 22

22) Assume you are growing bacteria on a lipid medium that started at pH 7. The action of bacterial lipases should cause the pH of the medium to

A) increase.

B) decrease.

C) stay the same.

back 22

Answer: B

front 23

23) Which of the following uses CO2 for carbon and H2 for energy?

A) chemoautotroph

B) chemoheterotroph

C) photoautotroph

D) photoheterotroph

back 23

Answer: A

front 24

24) Which of the following uses glucose for carbon and energy?

A) chemoautotroph

B) chemoheterotroph

C) photoautotroph

D) photoheterotroph

back 24

Answer: B

front 25

25) Which of the following has bacteriochlorophylls and uses alcohols for carbon?

A) chemoautotroph

B) chemoheterotroph

C) photoautotroph

D) photoheterotroph

back 25

Answer: D

front 26

26) Cyanobacteria are a type of

A) chemoautotroph.

B) chemoheterotroph.

C) photoautotroph.

D) photoheterotroph.

back 26

Answer: C

front 27

27) Which of the following statements are TRUE?

1-Electron carriers are located at ribosomes.

2-ATP is a common intermediate between catabolic and anabolic pathways.

3-ATP is used for the long-term storage of energy and so is often found in storage granules.

4-Anaerobic organisms are capable of generating ATP via respiration.

5-ATP can be generated by the flow of protons across protein channels.

A) 2, 4, 5

B) 1, 3, 4

C) 2, 3, 5

D) 1, 2, 3

E) All of the statements are true.

back 27

Answer: A

front 28

28) Microorganisms that catabolize sugars into ethanol and hydrogen gas would most likely be categorized as

A) aerobic respirers.

B) anaerobic respirers.

C) heterolactic fermenters.

D) homolactic fermenters.

E) alcohol fermenters.

back 28

Answer: C

front 29

29) Which of the following statements regarding metabolism is FALSE?

A) Heat may be released in both anabolic and catabolic reactions.

B) ATP is formed in catabolic reactions.

C) ADP is formed in anabolic reactions.

D) Anabolic reactions are degradative.

back 29

Answer: D

front 30

30) Which of the following is TRUE about this reaction?

A) This process requires O2.

B) This process occurs anaerobically.

C) This process requires the entire electron transport system.

D) This process requires light.

E) This process requires O2and the electron transport system.

back 30

Answer: B

front 31

31) Which of the following statements regarding the Entner-Doudoroff pathway is TRUE?

A) It involves glycolysis.

B) It involves the pentose phosphate pathway.

C) NADH is generated.

D) ATP is generated.

E) NADH and ATP are generated.

back 31

Answer: D

front 32

32) Assume you are working for a chemical company and are responsible for growing a yeast culture that produces ethanol. The yeasts are growing well on the maltose medium but are not producing alcohol. What is the most likely explanation?

A) The maltose is toxic.

B) O2is in the medium.

C) Not enough protein is provided.

D) The temperature is too low.

E) The temperature is too high.

back 32

Answer: B

front 33

The rates of O2 and glucose consumption by a bacterial culture are shown in Figure 5.6. Assume a bacterial culture was grown in a glucose medium without O2. Then O2 was added at the time marked X. The data indicate that

A) these bacteria don't use O2.

B) these bacteria get more energy anaerobically.

C) aerobic metabolism is more efficient than fermentation.

D) these bacteria cannot grow anaerobically.

back 33

Answer: C

front 34

34) An enzyme, citrate synthase, in the Krebs cycle is inhibited by ATP. This is an example of all of the following EXCEPT

A) allosteric inhibition.

B) competitive inhibition.

C) feedback inhibition.

D) noncompetitive inhibition.

back 34

Answer: B

front 35

35) If a cell is starved for ATP, which of the following pathways would most likely be shut down?

A) Kreb's cycle

B) glycolysis

C) pentose phosphate pathway

D) Krebs cycle and glycolysis

back 35

Answer: C

front 36

36) Which of the following statements regarding the glycolysis pathway is FALSE?

A) Two pyruvate molecules are generated.

B) Four ATP molecules are generated via substrate-level phosphorylation.

C) Two NADH molecules are generated.

D) One molecule of ATP is expended.

E) Two molecules of water are generated.

back 36

Answer: D

front 37

The graph at the left in Figure 5.7 shows the reaction rate for an enzyme at its optimum temperature. Which graph shows enzyme activity at a higher temperature?

A) a

B) b

C) c

D) d

back 37

Answer: B

front 38

38) A bacterial culture grown in a glucose-peptide medium causes the pH to increase. The bacteria are most likely

A) fermenting the glucose.

B) oxidizing the glucose.

C) using the peptides.

D) not growing.

back 38

Answer: C

front 39

39) Gallionella bacteria can get energy from the reaction Fe2+ → Fe3+. This reaction is an example of

A) oxidation.

B) reduction.

C) fermentation.

D) photophosphorylation.

E) the Calvin-Benson cycle.

back 39

Answer: A

front 40

40) In Figure 5.8, where is ATP produced?

A) a

B) b

C) c

D) d

E) e

back 40

Answer: E

front 41

41) Refer to Figure 5.8. In aerobic respiration, where is water formed?

A) a

B) b

C) c

D) d

E) e

back 41

Answer: D

front 42

42) In Figure 5.8, the structure labeled "1" is
A) NAD+.
B) ATP synthase.
C) a plasma membrane.
D) a cell wall.
E) cytoplasm.

back 42

Answer: C

front 43

43) In Figure 5.8, the path labeled "2" is the flow of

A) electrons.

B) protons.

C) energy.

D) water.

E) glucose.

back 43

Answer: B

front 44

44) What is the most acidic place in Figure 5.8?

A) a

B) b

C) c

D) d

E) e

back 44

Answer: A

front 45

45) A urease test is used to identify Mycobacterium tuberculosis because

A) urease is a sign of tuberculosis.

B) tuberculosis produces urease.

C) urea accumulates during tuberculosis.

D) some bacteria reduce nitrate ion.

E) bovis can cause tuberculosis.

back 45

Answer: B

front 46

46) Researchers are developing a ribozyme that cleaves the HIV genome. This pharmaceutical agent could be described as

A) an RNA molecule capable of catalysis.

B) a hydrolase.

C) a genetic transposable element.

D) a protease inhibitor.

E) a competitive inhibitor for reverse transcriptase.

back 46

Answer: A

front 47

47) Which statements correspond to amphibolic pathways?

  1. anabolic and catabolic reactions are joined through common intermediate
  2. shared metabolic pathways
  3. Feedback inhibition can help regulate rates of reactions
  4. both types of reactions are necessary but do not occur simultaneously

A) 1 only

B) 1, 2, 3

C) 1, 2, 3, 4

D) 2, 4

E) 2, 3, 4

back 47

Answer: B