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Microbiology chapter 3 pre-test

front 1

Which of the following pairs is correctly matched?

back 1

Atomic force microscopes & DNA

front 2

What does refraction mean?

back 2

A measure of the light-bending ability of medium

front 3

Place these structures of the compuound light microscope in the order that light passes through them on the way to the observer's eyes:
1) condenser
2) ocular lens
3) illuminator
4) specimen
5) objective lens

back 3

Illuminator -->condenser-->specimen-->objective lens (ocular lens).

front 4

What is the correct order for the Gram stain process?

back 4

crystal violet--> iodine--> decolorizer--> safranin

front 5

The acid-fast stain differs from the Gram stain procedure in the decolorizing agen used. What is the reagent used in an acid-fast stain, and why is it necessary?

back 5

Acid-alcohol is used to remove the primary stain from bacteria that do not have waxy cell walls.

front 6

A student completes a Gram stain on a bacterial smear that has a mix of gram-negative and gram positive organisms but forget to apply the safranin counterstain. What would be the outcome of the Gram stain?

back 6

The gram-positive organisms would appear purple, and the gram-negative organisms would be colorless.

front 7

Which of the following is NOT visible through a compund light microscope?

back 7

0.02-micrometer ribosome

front 8

In the Gram stain, what is the purpose of the counterstain?

back 8

To make gram-negative cells visible

front 9

Which of the following statements about electron microscopy & light microscopy is FALSE?

back 9

Both the electron micorscope and the light microscope use the same wavelengths for illumination.

front 10

In the Gram stain, what is the purpose of the decolorizer?

back 10

To selectively remove stain from cells

front 11

Before cells are stained, the specimen must be fixed. Fixing a specimen accomplishes all of the following EXCEPT which one?

back 11

Fixation differentiates the various cells being observed.

front 12

Which of the following staining procedures is matched with the principal use for that particular stain?

back 12

Endospore stain to distinguish dormant structures formed during adverse environmental conditions

front 13

What metric unit would be most appropriate for expressing the size of most viruses?

back 13

Nanometer

front 14

A student is observing a Gram stain on the 100x (oil immersion) lens. The image appears cloudy & dark. Which of the following actions will NOT increase the clarity of the image?

back 14

Lower the stage to increase the distance between the object & the objective lens.

front 15

Immersion oil is used with the 100x objective lens of the compound light microscope to ______.

back 15

prevent the loss of resolution due to refraction

front 16

Consider the following situations, and determine when a simple stain should be used instead of a Gram stain.

back 16

A microbiologist is trying to determine whether an organism has flagella.

front 17

If you use a compound light microscope, a 2-micrometer bacterial cell is best seen at which magnification?

back 17

1000x

front 18

Which of the following types of microscopy allows organisms to be identified based on antigens, even if the organisms are inside a cell?

back 18

Fluorescent microscopy

front 19

A student is observing an object that displays the internal detail on virus at great magnification. Which type of microscope is used for this image?

back 19

Transmission electron microscope

front 20

A microscopist is studying the surface pores on a bacterial specimen. Which microscope provides the best three-dimensional surface structure image?

back 20

Scanning electron microscope

front 21

Which of the following is the most convenient & appropriate unit for expressing the size of an average bacterial cell?

back 21

Micrometer

* Most bacteria fall in the range of 1-10 micrometers in size.