front 1 What structure does light pass through after leaving the condenser in a compound light microscope | back 1 specimen |
front 2 Which of the following pair is matched | back 2 a) methylene blue - simple stain b) acidic dye - capsule stain c) crystal violet - simple stain d) alcohol-acetone - descolorizer e) crystal violet - basic dye f) iodine- mordant g) carbolfuchsin - basic day |
front 3 Which of the following steps of the Gram stain are in correct order? | back 3 a) Crystal Violet b) Iodine c) Alcohol acetone d) Safranin |
front 4 Which of the following is true regarding the acid-fast stain | back 4 a) It is used to identify members of the genus Mycobacterium b) acid-fast cell retain the primary dye after treatment with acid-alcohol c) acid fast cell appear red in a completed acid fast stain d) non-acid-fast microbes appear blue in a completed acid-fast stain |
front 5 The purpose of a mordant in the Gram stain is to | back 5 prevent the crystal violet from leaving the cells |
front 6 Which of the following steps is correct regarding to specimen slide preparation? | back 6 a) making a smear b) fixing c) staining |
front 7 The negative stain is used to | back 7 visualize capsule |
front 8 Simple staining is necessary to improve contrast in which microscope? | back 8 compound lightt microscope |
front 9 Which microscope is used to see internal structures of cell in a natural state? | back 9 phase contrast microscope |
front 10 Which microscope uses visible light? | back 10 differential interference contrast (DIC) microscope |
front 11 Which microscope has the highest magnification and greatestt resolution? | back 11 electron microscope |
front 12 Which microscope produces an image of a light cell against a dark background; internal structures are not visible | back 12 darkfield microscope |
front 13 A virus measures 100 nm in length. What is its length in um? | back 13 0.01 um |
front 14 Which microscope is not useful for observing living cells? | back 14 scanning electron microscope (SEM) |
front 15 A microorganism measures 5 um in length. Its length in mm would be | back 15 0.005 mm |
front 16 Which of the following correctly traces the path of light through the compound microscope? | back 16 a) light source b) condenser c) specimen d) objective lens e) ocular lens |
front 17 Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light? | back 17 fluorescence microscope |
front 18 Which microscope is most useful for visualizing a BIOFILM? | back 18 scanning acoustic microscope (SAM) |
front 19 You are performing a Gram stain on gram positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point? | back 19 purple |
front 20 You are performing a Gram stain on gram negative bacteria and you stop after the addition of the mordant. What is the appearance of the bacteria at this point? | back 20 purple |
front 21 You are performing a Gram stain on gram negative bacteria and you stop after the descolorizer. What is the appearance of the bacteria at this point? | back 21 colorless |
front 22 You are performing a Gram stain on gram positive bacteria and you stop after the addition of the counterstain. What is the appearance of the bacteria at this point? | back 22 purple |
front 23 Which type of stain is useful in helping clinicians to decide which antibiotic to prescribe for a bacterial infection? | back 23 Gram stain |
front 24 What is the total magnification of a specimen viewed with a 10x ocular lens and a 40x objective lens? | back 24 400 |
front 25 Which of the following provides the lowest magnification ? | back 25 scanning electron microscope (SEM) |
front 26 Which microscope uses two beam of light to produce a three dimensional color image? | back 26 DIC microscope |
front 27 Which microscope is used to see intracellular detail in a living cell? | back 27 two-photon microscope |
front 28 Which microscope is used to observe viruses and the internal structure of thinly sectioned cells? | back 28 transmission electron microscope (TEM) |
front 29 Which step in the Gram stain is the critical step in differentiating gram positive cells from gram negative cell? | back 29 alcohol-acetone |
front 30 You find colorless areas in cell in a gram stained smear. What should you apply next? | back 30 an endospore stain |
front 31 Which microscope is used for observing the surfaces of cell and viruses | back 31 scanning electron microscope (SEM) |
front 32 The resolution of a microscope can be improved by changing the | back 32 wavelength of light |
front 33 The term resolution refers to | back 33 the ability to distinguish fine structure and detail in a specimen |
front 34 The courterstain used in the Gram stain is a basic dye | back 34 True |
front 35 Both phase-contrast microscopy and differential interference contrast microscopy are used to view the internal structures of cells without staining | back 35 True |
front 36 The capsules and flagella of bacteria can be observed in gram stained smears | back 36 False |
front 37 Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, molecules or DNA | back 37 True |