Sac 3: 10% NaCl in distilled water

NaCl diffused from the sac into the water; silver nitrate added to the water showed the presence of Cl–. Osmosis caused water to enter the sac because the solution in the sac was hypertonic to the distilled water in the beaker.

Sac 4: 40% sucrose and Congo red dye in distilled water

The Congo red dye did not diffuse from the sac into the water; the water in the beaker did not turn red. The sucrose did not diffuse from the sac; upon boiling, some of the sucrose bonds are hydrolyzed, releasing glucose and fructose. Using Benedict’s test then indicates the presence of glucose if sucrose passed through the membrane; the Benedict’s test was negative. Water moved into the sac by osmosis; the sac gained weight.

What single characteristic of the differentially permeable membranes used in the laboratory determines the substances that can pass through them? In addition to this characteristic, what other factors influence the passage of substances through living membranes?

Size of pores. Solubility in the lipid portion of the membrane and/or presence of membrane “carriers” for the substance(s).

A semipermeable sac containing 4% NaCl, 9% glucose, and 10% albumin is suspended in a solution with the following com- position: 10% NaCl, 10% glucose, and 40% albumin. Assume that the sac is permeable to all substances except albumin. State whether each of the following will (a) move into the sac, (b) move out of the sac, or (c) not move.

glucose: a, moves into sac
albumin:c, does not move
water: b, moves out of sac
NaCl: a, moves into sac

Diffusion through living membranes—the egg

Egg 1 in distilled water:
Water moves by osmosis from an area of higher water concentration into an area of lower water concentration.
Egg 2 in 30% sucrose:
solution. Water moves by osmosis from an area of higher water concentration into an area of lower water concentration.

Microscope fields containing RBCs. Arrows show the direction of net osmosis.
Which field contains a hypertonic solution? _ The cells in this field are said to be _________. Which field contains an isotonic bathing solution? _ Which field contains a hypotonic solution? _ What is happening to the cells in this field? _________

- C. Hypertonic

- Crenated

- B. Isotonic

- A. Hypotonic

- Cells take on water by osmosis until they become bloated and burst in a hypotonic solution. Hemolysis, bursting as excessive water entry occurs

The faster-diffusing gas is _________________.

NH4OH ammonium hydroxide

The precipitate forms closer to the _________________ end.

HCl hydrochloric acid

What determines whether a transport process is active or passive?

Whether or not the cell must provide ATP for the process; if so, the process is active.

Passive process

- account for the movement of fats and respiratory gases through the plasma membrane

- include osmosis, simple diffusion, and filtration

- use hydrostatic pressure or molecular energy as the driving force

Active process

- explain solute pumping, phagocytosis, and pinocytosis

- may occur against concentration and/or electrical gradients

- move ions, amino acids, and some sugars across the plasma membrane

For the osmometer demonstration, explain why the level of the water column rose during the laboratory session.

The thistle tube was immersed in a dialysis sac which, in turn, was immersed in water. Since water will move down its concentration gradient if it is able, water diffused from the beaker into the sac, where its concentration was much lower.
As a result, the fluid column (molasses and entering water) rose in the thistle tube.

Selective permeability

Some molecules can cross. but others cannot

Diffusion

Movement of molecules from a region of their higher concentration to an area where they are in lower concentration

Simple diffusion

Movement of molecules from a region of their higher concentration to a lower region of their concentration. Its driving force is kinetic energy of the molecules themselves.

Facilitated diffusion

Movement of molecules across cell membranes in response to a concentration gradient with the aid of a membrane protein

Osmosis

Diffusion of water through a semipermeable or differentially permeable membrane. Water moves from an area of higher water concentration to an area of lower water concentration, from hypotonic to hypertonic solution

Filtration

Passage of substances across a membrane from an area of higher hydrostatic pressure to an area of lower hydrostatic pressure

Vesicular transport

The movement of vesicles within the cell with the aid of the cytoplasm

Endocytosis

Large molecules or particles move into cells

Exocytosis

Intracellular vesicles more to the cell membrane and then release their contents to the extracellular fluid