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Exercise 2-14 Micro Lab: Chemical Germicides - Disinfectants and Antiseptics

front 1

Chemical germicides

back 1

Substances designed to reduce the number of pathogens on a surface, in a liquid, or in living tissue

front 2

Disinfectants

back 2

Germicides designed for use on surfaces like floors, tables, sinks, countertops, surgical instruments, or liquids are called disinfectants

front 3

Antiseptics

back 3

Germicide's designed for use on or in living tissue

front 4

Use dilution test

back 4

A standard procedure used to measure the effectiveness of disinfectants specifically against staff, salmonella, pseudomonas, cholerasuis

front 5

What is the standard procedure for the use dilution and test

back 5

And the standard procedure, glass beads or stainless steel cylinders coated with living bacteria are exposed to varying concentrations, dilutions, of test germicides then transferred to a growth medium. After a period of incubation, the medium is examined for growth. If a solution is sufficient to prevent microbial growth at least 95% of the time, it meets the required standards and is considered a usable dilution of that germicide for a specific application.

front 6

Which disinfectants and which antiseptics will we be using in the exercise?

back 6

The disinfectants selected are household bleach and Lysol brand 2 disinfectant. The antiseptics are hydrogen peroxide and isopropyl alcohol.

front 7

If, during exposure to the germicide, all of the bacteria on the bead are killed, what happens to the inoculated broth?

back 7

The broth inoculated with that bead will remain clear

front 8

What happens if any of the bacteria survive the germicide exposure?

back 8

They will be produced during incubation and make the broth turbid