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Instructions for Side by Side Printing
  1. Print the notecards
  2. Fold each page in half along the solid vertical line
  3. Cut out the notecards by cutting along each horizontal dotted line
  4. Optional: Glue, tape or staple the ends of each notecard together
  1. Verify Front of pages is selected for Viewing and print the front of the notecards
  2. Select Back of pages for Viewing and print the back of the notecards
    NOTE: Since the back of the pages are printed in reverse order (last page is printed first), keep the pages in the same order as they were after Step 1. Also, be sure to feed the pages in the same direction as you did in Step 1.
  3. Cut out the notecards by cutting along each horizontal and vertical dotted line
To print: Ctrl+PPrint as a list

35 notecards = 9 pages (4 cards per page)

Viewing:

Exercise 3: The Microscope

front 1

LABEL ALL INDICATED PARTS OF THE MICROSCOPE.

back 1

front 2

LABEL ALL INDICATED PARTS OF THE MICROSCOPE.

back 2

front 3

LABEL ALL INDICATED PARTS OF THE MICROSCOPE.

back 3

front 4

LABEL ALL INDICATED PARTS OF THE MICROSCOPE.

back 4

front 5

LABEL ALL INDICATED PARTS OF THE MICROSCOPE.

back 5

front 6

EXPLAIN THE PROPER TECHNIQUE FOR TRANSPORTING THE MICROSCOPE.

back 6

WHEN TRANSPORTING THE MICROSCOPE, HOLD IT IN AN UPRIGHT POSITION WITH ONE HAND ON ITS ARM AND THE OTHER SUPPORTING ITS BASE. AVOID SWINGING THE INSTRUMENT DURING ITS TRANSPORT AND JARRING THE INSTRUMENT WHEN SETTING IT DOWN.

front 7

THE FOLLOWING STATEMENTS ARE TRUE OR FALSE. IF TRUE, WRITE T ON THE ANSWER BLANK. IF FALSE, CORRECT THE STATEMENT BY WRITING ON THE BLANK THE PROPER WORD OR PHRASE TO REPLACE THE ONE THAT IS UNDERLINED. *(UNDERLINE WORDS WILL BE IN THESE)

1. THE MICROSCOPE LENS MAY BE CLEANED (WITH ANY SOFT TISSUE).

back 7

F: FALSE; ONLY WITH SPECIAL GRIT-FREE LENS PAPER

front 8

THE FOLLOWING STATEMENTS ARE TRUE OR FALSE. IF TRUE, WRITE T ON THE ANSWER BLANK. IF FALSE, CORRECT THE STATEMENT BY WRITING ON THE BLANK THE PROPER WORD OR PHRASE TO REPLACE THE ONE THAT IS UNDERLINED. *(UNDERLINE WORDS WILL BE IN THESE)

2. THE MICROSCOPE SHOULD BE STORED WITH THE (OIL IMMERSION) LENS IN POSITION OVER THE STAGE.

back 8

F: FALSE; LOWEST-POWER OBJECTIVE

front 9

THE FOLLOWING STATEMENTS ARE TRUE OR FALSE. IF TRUE, WRITE T ON THE ANSWER BLANK. IF FALSE, CORRECT THE STATEMENT BY WRITING ON THE BLANK THE PROPER WORD OR PHRASE TO REPLACE THE ONE THAT IS UNDERLINED. *(UNDERLINE WORDS WILL BE IN THESE)

3. WHEN BEGINNING TO FOCUS, USE THE (LOWEST-POWER) LENS.

back 9

TRUE

front 10

THE FOLLOWING STATEMENTS ARE TRUE OR FALSE. IF TRUE, WRITE T ON THE ANSWER BLANK. IF FALSE, CORRECT THE STATEMENT BY WRITING ON THE BLANK THE PROPER WORD OR PHRASE TO REPLACE THE ONE THAT IS UNDERLINED. *(UNDERLINE WORDS WILL BE IN THESE)

4. WHEN FOCUSING, ALWAYS FOCUS (TOWARD) THE SPECIMEN.

back 10

F:FALSE; AWAY (SO YOU WONT GET TO CLOSE AND BREAK THE SLIDE)

front 11

THE FOLLOWING STATEMENTS ARE TRUE OR FALSE. IF TRUE, WRITE T ON THE ANSWER BLANK. IF FALSE, CORRECT THE STATEMENT BY WRITING ON THE BLANK THE PROPER WORD OR PHRASE TO REPLACE THE ONE THAT IS UNDERLINED. *(UNDERLINE WORDS WILL BE IN THESE)

5. A COVERSLIP SHOULD ALWAYS BE USED (WITH WET MOUNTS AND THE HIGH-POWER AND OIL LENSES).

back 11

F:FALSE; WITH WET MOUNTS

front 12

PLATFORM ON WHICH THE SLIDE RESTS FOR VIEWING

back 12

I. STAGE

front 13

USED TO INCREASE THE AMOUNT OF LIGHT PASSING THROUGH THE SPECIMEN

back 13

B. CONDENSER

front 14

SECURE(S) THE SLIDE TO THE STAGE

back 14

E. MECHANICAL STAGE OR SPRING CLIPS

front 15

DELIVERS A CONCENTRATED BEAM OF LIGHT TO THE SPECIMEN

back 15

D. IRIS DIAPHRAGM

front 16

USED FOR PRECISE FOCUSING ONCE INITIAL FOCUSING HAS BEEN DONE

back 16

C. FINE ADJUSTMENT KNOB

front 17

CARRIES THE OBJECTIVE LENSES; ROTATES SO THAT THE DIFFERENT OBJECTIVE LENSES CAN BE BROUGHT INTO POSITION OVER THE SPECIMEN

back 17

F. MOVABLE NOSE-PIECE

front 18

DEFINE VIRTUAL IMAGE

back 18

THE REAL IMAGE IS MAGNIFIED BY THE OCULAR LENS TO PRODUCE THE VIRTUAL IMAGE SEEN BY YOUR EYE.

front 19

DEFINE RESOLUTION

back 19

(RESOLVING POWER), THAT IS, THE ABILITY TO DISCRIMINATE TWO CLOSE OBJECTS AS SEPARATE.

front 20

THE DISTANCE FROM THE BOTTOM OF THE OBJECTIVE LENS IN USE TO THE SPECIMEN IS CALLED THE _________.

back 20

WORKING DISTANCE

front 21

ASSUME THERE IS AN OBJECT ON THE LEFT SIDE OF THE FIELD THAT YOU WANT TO BRING TO THE CENTER (THAT IS, TOWARD THE APPARENT RIGHT). IN WHAT DIRECTION WOULD YOU MOVE YOUR SLIDE?

back 21

TO THE RIGHT

front 22

THAT AREA OF THE SPECIMEN SEEN WHEN LOOKING THROUGH THE MICROSCOPE IS THE ___________.

back 22

FIELD

front 23

IF A MICROSCOPE HAS A 10X OCULAR AND THE TOTAL MAGNIFICATION AT THE PARTICULAR TIME IS 950X, THE OBJECTIVE LENS IS USE AT THE TIME IS _______X.

back 23

95

front 24

WHY SHOULD THE LIGHT BE DIMMED WHEN LOOKING AT LIVING (NEARLY TRANSPARENT) CELLS?

back 24

TO PROVIDE MORE CONTRAST FOR VIEWING THE LIGHTLY STAINED CELLS; AND A WET MOUNT WILL DRY OUT QUICKLY IN BRIGHT LIGHT BECAUSE A BRIGHT LIGHT SOURCE IS HOT (CAN DAMAGE THE CELLS)

front 25

IF, AFTER FOCUSING IN LOW POWER, ONLY THE FINE ADJUSTMENT NEED BE USED TO FOCUS THE SPECIMEN AT THE HIGHER POWERS, THE MICROSCOPE IS SAID TO BE ____________.

back 25

PARFOCAL

front 26

IF, WHEN USING A 10X OCULAR AND A 15X OBJECTIVE, THE FIELD SIZE IS 1.5 MM, THE APPROXIMATE FIELD SIZE WITH A 30X OBJECTIVE IS __________MM.

back 26

0.75 MM

front 27

IF THE SIZE OF THE HIGH-POWER FIELD IS 1.2 MM, AND OBJECT THAT OCCUPIES APPROXIMATELY A THIRD OF THAT FIELD HAS AN ESTIMATED DIAMETER OF _______MM.

back 27

0.67

front 28

SAY YOU ARE OBSERVING AN OBJECT IN THE LOW-POWER FIELD. WHEN YOU SWITCH TO HIGH-POWER, IT IS NO LONGER IN YOUR FIELD OF VIEW. WHY MIGHT THIS OCCUR? WHAT SHOULD BE DONE INITIALLY TO PREVENT THIS FROM HAPPENING?

back 28

OCCURS BECAUSE IN LOWER POWER YOU HAVE A WIDER FIELD OF VIEW AND WHEN YOU SWITCH TO A HIGHER POWER IT NARROWS YOUR VIEW AND IT BECOMES OUT OF FOCUS WHICH DOES NOT HELP.

WAYS TO PREVENT IS: CENTER THE OBJECT YOU WANT TO VIEW AS MUCH AS POSSIBLE BEFORE SWITCHING TO A HIGHER POWER AND INCREASE MAGNIFICATION SLOWLY.

front 29

DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE?

RESOLUTION __________________________

back 29

INCREASE

front 30

DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE?

WORKING DISTANCE ____________________________

back 30

DECREASE

front 31

DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE?

AMOUNT OF LIGHT NEEDED ________________________

back 31

INCREASE

front 32

DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE?

DEPTH OF FIELD ________________________________

back 32

DECREASE

front 33

A STUDENT HAS THE HIGH-DRY LENS IN POSITION AND APPEARS TO BE INTENTLY OBSERVING THE SPECIMEN. THE INSTRUCTOR, NOTING A WORKING DISTANCE OF ABOUT 1 CM, KNOWS THE STUDENT ISN'T ACTUALLY SEEING THE SPECIMEN. HOW SO?

back 33

A HIGH-DRY LENS IS A HIGH POWER, NON-OIL IMMERSION LENS. A HIGH POWER LENS WOULD HAVE A MUCH SHORTER WORKING DISTANCE THEN 1 CM. IT WOULD BE MORE LIKE A COUPLE OF MM, AT THIS POINT THE STUDENT IS LOOKING AT DUST AND IS WAY OUT OF FOCUS

front 34

DESCRIBE THE PROPER PROCEDURE FOR PREPARING A WET MOUNT.

back 34

1. THE OBJECT IS PLACED IN A DROP OF WATER (OR SALINE) ON A CLEAN SLIDE.
2. A COVER-SLIP IS HELD AT A 45 DEGREE ANGLE WITH THE FINGERTIPS.
3. IT IS LOWERED CAREFULLY OVER THE WATER AND THE OBJECT.

front 35

INDICATE THE PROBABLE CAUSE OF THE FOLLOWING SITUATIONS ARISING DURING USE OF A MICROSCOPE.

A. ONLY HALF OF THE FIELD IS ILLUMINATED.

B. FIELD DOES NOT CHANCE AS MECHANICAL STAGE IS MOVED.

back 35

A. BLOCKAGE IN THE LIGHT PATH.

B. MECHANICAL CONTACT BETWEEN THE SPECIMEN AND THE OBJECTIVE LENS