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Microbiology Lab Experiments- Exercise 20

front 1

The effect of temperature can be determined by measuring the _________________________.

back 1

population growth rate

front 2

When they speak of "air" as a growth requirement what do they mean?

back 2

they are referring to the oxygen in air

front 3

When they refer to oxygen they mean _______________.

back 3

molecular oxygen that acts as an electron acceptor in aerobic respiration.

front 4

What is the an image of?

back 4

bacterial growth rate

front 5

During log phase the cells are growing at ____________________________.

back 5

Fastest rate possible under the conditions provided

front 6

What is generation or doubling time?

back 6

The time it takes for one cell to divide into 2 cells or the time required for the population of cells to double.

front 7

The shorter the generation time the ___________ the growth.

back 7

faster

front 8

Turbidity can be measured with a ______________.

back 8

spectrophotometer to determine growth

front 9

True or false. Although turbidity is not a direct measure of bacterial numbers, increasing turbidity does indicate growth.

back 9

true

front 10

Changes in the logarithmic absorbance scale on the spectrophotometer correspond to changes in ______________________________.

back 10

The number of cells

front 11

A growth curve can be obtained by graphing absorbance (Y-axis)vs. time (X-axis). The rate of growth is indicated by the ____________ of the lines, faster growth produces a steeper (higher number) slope.

back 11

slope

front 12

What is the minimum growth temperature?

back 12

the lowest temperature at which a species will grow

front 13

A species grows fastest at its ___________________?

back 13

optimum growth temperature

front 14

The highest temperature at which a species will grow is its ____________________________.

back 14

maximum growth temperature

front 15

At temperatures near the maximum growth temperature, growth ceases, presumably because of the ______________________________.

back 15

denaturation of enzymes

front 16

Increasing temperature optimizes or lowers the growth of the bacteria?

back 16

lowers

front 17

At what temperature would you expect E-coli to grow best?

back 17

35 degrees Celsius

front 18

Define the optimal growth temperature for each of the following classes of bacteria...

back 18

hyperthermophile 80 and above, Thermophile between 50 and 80. Mesophile between 20 and 50. Psychrophile -5 to 20.

front 19

What is the optimum growth temperature for most human pathogens?

back 19

37* is the optimum temperature for most pathogens because that is human body temperature.