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Laboratory Experiments in Microbiology-Exercise 4

front 1

Agar deep is a semi-solid or solid?

back 1

semi-solid

front 2

Describe the growth pattern of a motile organism in a semisolid deep.

back 2

Moved upwards spread out near top where it is oxygen rich- the bacteria is nutrient rich

front 3

How will the arrangement of Lactococcus lactis in broth differ from the arrangement in the slant culture?

back 3

in the broth it will be turbid, flocculent, pellicle or sediment because it is in liquid
and it is oxygen rich
the slant will be zig-zagged and less growth at top

front 4

Results of nutrient broth:

back 4

Lactococcus lactis:turbid -yes; flocculent or pellicle-no; pigment-no
Pseudomonas aeruginosa-yes, pellicle and sediment and no pigment
Proteus vulgaris-yes, no, no

front 5

Nutrient agar slant results:

back 5

Lactococcus lactis- flocculent
Pseudomonas aeruginosa-green down side
Proteus vulgaris- flocculent all over

front 6

Nutrient solid agar deep:

back 6

Lactococcus lactis- squiggly down stab mark
Pseudomonas aeruginosa-motile and flocculent and pellicle
Proteus vulgaris- aerobic and stab mark

front 7

What are agar slants used for?

back 7

provide solid growth surface

front 8

Did growth occur at different levels of the agar deep?

back 8

yes