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BCH 100 Lecture 4: Amino Acids 2

front 1

Arginine has a ________ group

back 1

Guanidino (A really strong base/ very high pKa)

front 2

Lysine has an _______ group

back 2

Amine

front 3

Histidine has an _______ group

back 3

Imidazole (Deprotonation occurs at a very low pH)

front 4

Can Zwitterions Act as Acids or Bases?

back 4

Yes they can

front 5

Which Amino Acids are ionizable?

back 5

Aspartate, Arginine, Tyrosine, Histidine, Glutamate, Cysteine, and Lysine

front 6

How do you calculate the Isoelectric point?

back 6

We need to find the pK values on either side of the Zwitterion. First, draw out all the ionizable amino acids. Then, write out their pKa values and rank them from 1 to however many. Then as you move through the number line of charges, note down which pKa takes the charge to 0 and from 0. Add those two values together and divide by 2 to find the isoelectric group.