front 1 The counterstain in the acid-fast stain is | back 1 a basic dye |
front 2 Which microscope is used to see internal structures of cells in a natural state? | back 2 Phase-contrast microscope |
front 3 Which microscope takes advantage of differences in the refractive indexes of cell structures? | back 3 Phase contrast microscope |
front 4 The appearance of gram-positive bacteria after addition of the first dye in the Gram stain. | back 4 purple |
front 5 Which microscope uses two beams of light to produce a three-dimensional, color image? | back 5 DIC microscope |
front 6 The purpose of the ocular lens is to | back 6 Magnify the image from the objective lens |
front 7 The appearance of gram-negative bacteria after addition of the mordant in the Gram stain. | back 7 purple |
front 8 Which of the following microscopes uses visible light? | back 8 DIC |
front 9 In this microscope, the observer does NOT look at an image through a lens. | back 9 electron microscope |
front 10 Cells are differentiated after which step in the Gram stain? | back 10 alcohol acetone |
front 11 The counterstain in the Gram stain is | back 11 a basic dye |
front 12 In Figure 3.1, line "a." points to the microscope's | back 12 ocular lens |
front 13 What is the total magnification of a chloroplast viewed with a 10x ocular lens and a 45x objective lens? | back 13 450x |
front 14 The appearance of gram-positive bacteria after adding the counterstain in the Gram stain. | back 14 purple |
front 15 In which microscope does the image look like a negative stain? | back 15 Darkfield microscope |
front 16 Which microscope achieves the highest magnification and greatest resolution? | back 16 electron microscope |
front 17 Which microscope is used to see intracellular detail in a living cell? | back 17 two-photon microscope |
front 18 Place the following steps in the correct sequence: | back 18 2,3,1 |
front 19 The appearance of gram-negative bacteria after addition of the decolorizing agent in the Gram stain. | back 19 colorless |
front 20 Which of the following pairs is mismatched? | back 20 Fluorescence microscope - uses a fluorescent light |
front 21 Place the steps of the Gram stain in the correct
order: | back 21 2-4-1-3 |
front 22 This microscope produces an image of a light cell against a dark background; internal structures are NOT visible. | back 22 darkfield microscope |
front 23 Which microscope is used to see detail of a 300-nm virus? | back 23 electron microscope |
front 24 What Gram reaction do you expect from acid-fast bacteria? | back 24 Gram-positive |
front 25 The resolution of a microscope can be improved by changing the | back 25 wavelength of light |
front 26 In Figure 3.1, line "b." points to the microscope's | back 26 objective lens |
front 27 The purpose of a mordant in the Gram stain is | back 27 to prevent the crystal violet from leaving the cells |
front 28 Bacterial smears are fixed before staining to | back 28 affix the cells to the slide and kill the bacteria |
front 29 Simple staining is often necessary to improve contrast in this microscope. | back 29 compound light microscope |
front 30 You find colorless areas in cells in a Gram-stained smear. What should you do next? | back 30 an endoscope stain |
front 31 What structure does light pass through after leaving the condenser in a compound light microscope? | back 31 specimen |
front 32 Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are | back 32 Endospores |
front 33 The best use of a negative stain is | back 33 to determine cell size and to determine cell shape |
front 34 In Figure 3.1, line "c." points to the microscope's | back 34 condenser |
front 35 Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light? | back 35 fluorescence microscope |