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week 10 quiz

front 1

Which structures are less susceptible to UV damage?

  • Fungal spores
  • Endospores
  • Protozoan cysts
  • Vegetative bacterial cells

back 1

  • Endospores
  • Protozoan cysts

front 2

Which of the following would be appropriately sterilized using UV radiation?

  • flask of nutrient broth
  • stack of plastic petri dishes, prior to pouring media
  • stainless steel surface in biological safety cabinet used for media preparation
  • nutrient agar plates with lids

back 2

  • stainless steel surface in biological safety cabinet used for media preparation

front 3

Which of the following DNA sequences would be the most likely to be damaged by UV radiation?

  • CTTGAATTAA
  • GCATGACATA
  • ATGCACATGA
  • GACACGTAGA

back 3

  • CTTGAATTAA

front 4

How is an antiseptic different from a disinfectant?

  • An antiseptic is used to remove or kill microorganisms IN the body, whereas a disinfectant is used to remove or kill microorganisms on the body surfaces.
  • An antiseptic kills or inactivates all microbial forms but a disinfectant does not destroy endospores.
  • There is essentially no difference and these terms can be used interchangeably.
  • An antiseptic is used to remove or kill microorganisms on tissue, whereas a disinfectant is used to remove or kill microorganisms on inanimate objects.

back 4

  • An antiseptic is used to remove or kill microorganisms on tissue, whereas a disinfectant is used to remove or kill microorganisms on inanimate objects.

front 5

How are agar plates inoculated for disk diffusion testing of antiseptics and disinfectants?

  • A loop is used to prepare a 4-way quadrant streak
  • A loop is used to prepare a lawn of growth by inoculating the entire surface of the plate
  • A swab is used to inoculate the entire surface of the plate, producing a lawn of growth
  • A four-way quadrant streak is done using a swab

back 5

  • A swab is used to inoculate the entire surface of the plate, producing a lawn of growth.

front 6

UV light has ______ wavelengths than visible light waves, and its peak energy is at a wavelength of ______.

  • longer; 254nm
  • longer; 100nm
  • shorter; 100nm
  • shorter; 254nm

back 6

  • shorter; 254nm

front 7

Place these types of bacteria in order of their susceptibility to chemical disinfection, from least susceptible to most susceptible.

  • Gram-positive bacteria
  • Acid-fast bacteria
  • Gram-negative bacteria
  • Bacterial endospores

back 7

  1. Bacterial endospores
  2. Acid-fast endospores
  3. Gram-negative bacteria
  4. Gram-positive bacteria

front 8

What two factors increase the effectiveness of a disinfectant on microorganisms?

  • Concentration of disinfectant
  • Time of exposure
  • Material used to apply disinfectant
  • Corrosiveness of the disinfectant

back 8

  • Concentration of disinfectant
  • Time of exposure

front 9

Which of the following are limitations of using UV radiation for disinfection?

  • UV radiation does not penetrate through paper, plastics, or liquids
  • UV radiation damages plastics and other nonmetal materials
  • UV radiation destroys bacteria but not viruses
  • UV radiation can damage the skin and eyes

back 9

  • UV radiation does not penetrate through paper, plastics, or liquids
  • UV radiation can damage the skin and eyes

front 10

The clear area of devoid of bacterial growth around an antiseptic or disinfectant disk is referred to as the

  • zone of death
  • no growth zone
  • death zone
  • zone of inhibition

back 10

  • zone of inhibiton

front 11

When two adjacent thymine molecules are joined by a covalent bond as a result of exposure to UV light, this structure is referred to as a

  • thymine dimer
  • thymine couplet
  • thymine duplex
  • thymine doublet

back 11

  • thymine dimer

front 12

Which of the following infectious agents is least likely to be damaged by UV exposure?

  • bacteria
  • viruses
  • prions
  • fungi
  • protozoa

back 12

  • prions

front 13

Place these steps in the order in which they will be performed when setting up a disk diffusion assay to test the effectiveness of disinfectants and antiseptics.

  • Using a swab, inoculate a suspension of the bacteria being tested onto an agar plate, spreading the inoculum over the entire surface.
  • Saturate sterile filter paper disks with the test chemical (disinfectant or antiseptic) and place onto the inoculated agar plate. Repeat with each chemical being tested.
  • Observe plates to determine the presence of a zone of inhibition around each antiseptic or disinfectant disk.
  • Incubate inoculated agar plates overnight to promote bacterial growth.

back 13

  1. Using a swab, inoculate a suspension of the bacteria being tested onto an agar plate, spreading the inoculum over the entire surface.
  2. Saturate sterile filter paper disks with the test chemical (disinfectant or antiseptic) and place onto the inoculated agar plate. Repeat with each chemical being tested.
  3. Incubate inoculated agar plates overnight to promote bacterial growth.
  4. Observe plates to determine the presence of a zone of inhibition around each antiseptic or disinfectant disk.

front 14

Which of the following would affect the size of a zone of inhibition around an antiseptic or disinfectant disk?

  • The susceptibility of the microorganism to the chemical
  • The concentration of chemical in the disk
  • The cellular target of the chemical
  • How quickly the chemical diffuses through the agar
  • The depth of the agar

back 14

  • The concentration of chemical in the disk
  • How quickly the chemical diffuses through the agar
  • The depth of the agar
  • The susceptibility of the microorganism to the chemical