Campbell Biology: Exam 3: Ch. 17: From Gene to Protein Flashcards

What is the start codon?

What are the stop codons?

During translation, nucleotide base triplets (codons) in mRNA are read in sequence in the 5’ → 3’ direction along the mRNA. Amino acids are specified by the string of codons. What amino acid sequence does the following mRNA nucleotide sequence specify?

5′−AUGGCAAGAAAA−3′

Express the sequence of amino acids using the three-letter abbreviations, separated by hyphens (e.g., Met-Ser-Thr-Lys-Gly).

Which amino acid does the codon GCA code for?

To identify the amino acids specified by the mRNA sequence, you first need to subdivide the sequence into codons of three nucleotides each. This can be done by placing a space between each codon. Which of the following is the correct division of the codons for the sequence given? Look for the correct placement of spaces.

A. 5′−A UGG CAA GAA AA−3′

B. 5′−AU GGC AAG AAA A−3′

C. 5′−AUG GCA AGA AAA−3′

D. 5′−AUGG CAA GAA AA−3′

E. 5′−AU GGCA AGAA AA−3′

The role of DNA in determining amino acid sequences

Before a molecule of mRNA can be translated into a protein on the ribosome, the mRNA must first be transcribed from a sequence of DNA.

What amino acid sequence does the following DNA nucleotide sequence specify?

3′−TACAGAACGGTA−5′

Express the sequence of amino acids using the three-letter abbreviations, separated by hyphens (e.g., Met-Ser-His-Lys-Gly).

Steps to convert a DNA sequence into an amino acid sequence.

1. First, transcribe the DNA sequence to determine the mRNA sequence. Be sure to remember the following:
   • The mRNA strand is complementary to the DNA strand.
   • Uracil (U) takes the place of thymine (T) in RNA to pair with A on the DNA.
   • The RNA is assembled in an antiparallel direction to the template strand of DNA. A 3’→ 5’ direction in DNA is transcribed in a 5’ → 3’ direction in RNA.
2. Next, subdivide the mRNA sequence into the individual three-letter codons in the 5’ to 3’ direction.
3. Then, refer to the table of codons to identify the three-letter abbreviation for the amino acid that corresponds to each codon.

The flow of information in a cell proceeds in what sequence?

A. from DNA to RNA to protein

B. from RNA to DNA to protein

C. from RNA to protein to DNA

D. from DNA to protein to RNA

E. from protein to RNA to DNA

A codon consists of _____ bases and specifies which _____ will be inserted into the polypeptide chain.

A. four ... fatty acid

B. two ... nucleotide

C. three ... nucleotide

D. three ... amino acid

E. four ... amino acid

A particular triplet of bases in the template strand of DNA is 5' AGT 3'. The corresponding codon for the mRNA transcribed is _____.

A. 5' TCA 3'

B. 3' UGA 5'

C. 3' ACU 5'

D. 3' UCA 5'

The figure above shows a simple metabolic pathway. According to Beadle and Tatum's hypothesis, how many genes are necessary for this pathway?

A. 1

B. 2

C. 3

D. It cannot be determined from the pathway.

Refer to the metabolic pathway illustrated above. If A, B, and C are all required for growth, a strain that is mutant for the gene-encoding enzyme A would be able to grow on medium supplemented with _____.

A. nutrient A only

B. nutrient B only

C. nutrient C only

D. nutrients A and C

In the process of transcription, _____.

A. DNA is replicated

B. mRNA attaches to ribosomes

C. RNA is synthesized

D. proteins are synthesized

Which of the following specifies a single amino acid in a polypeptide chain?

A. the three-base sequence of mRNA

B. the complementarity of DNA and RNA

C. the aminoacyl-tRNA synthetase

D. the base sequence of the tRNA

In the diagram, the gray unit represents _____.

A. RNA

B. DNA

C. transcription factors

D. RNA polymerase

E. The promoter

In the diagram, the green unit represents _____.

A. RNA

B. DNA

C. transcription factors

D. RNA polymerase

E. The promoter

In the diagram below, the two blue strands represent _____.

A. RNA

B. DNA

C. transcription factors

D. RNA polymerase

E. The promoter

Which of these correctly illustrates the pairing of DNA and RNA nucleotides?

The direction of synthesis of an RNA transcript is _____.

A. 1' —> 5'

B. 5' —> 3'

C. 1' —> 3'

D. 3' —> 5'

E. 2' —> 4'

Where does RNA polymerase begin transcribing a gene into mRNA?

A. It starts at one end of the chromosome.

B. The ribosome directs it to the correct portion of the DNA molecule.

C. Transfer RNA acts to translate the message to RNA polymerase.

D. It starts after a certain nucleotide sequence called a promoter.

E. It looks for the AUG start codon.

Transcription in eukaryotes requires which of the following in addition to RNA polymerase?

A. aminoacyl-tRNA synthetase

B. several transcription factors

C. start and stop codons

D. ribosomes and tRNA

During RNA processing a(n) _____ is added to the 5' end of the RNA.

A. 3' untranslated region

B. a long string of adenine nucleotides

C. 5' untranslated region

D. coding segment

E. modified guanine nucleotide

During RNA processing a(n) _____ is added to the 3' end of the RNA.

A. 3' untranslated region

B. a long string of adenine nucleotides

C. 5' untranslated region

D. coding segment

E. modified guanine nucleotide

Spliceosomes are composed of _____.

A. snRNPs and other proteins

B. polymerases and ligases

C. introns and exons

D. the RNA transcript and protein

E. snRNPs and snurps

The RNA segments joined to one another by spliceosomes are _____.

A. caps

B. exons

C. snRNPs

D. tails

E. introns

Translation occurs in the _____.

A. cytoplasm

B. lysosome

C. nucleus

D. Golgi apparatus

E. nucleoplasm

After an RNA molecule is transcribed from a eukaryotic gene, what are removed and what are spliced together to produce an mRNA molecule with a continuous coding sequence?

A. silencers ... enhancers

B. promoters ... operators

C. exons ... introns

D. operators ... promoters

E. introns ... exons

Alternative RNA splicing _____.

A. increases the rate of transcription

B. can allow the production of proteins of different sizes and functions from a single gene

C. can allow the production of similar proteins from different RNAs

D. is a mechanism for increasing the rate of translation

Use this model of a eukaryotic transcript to answer the following question. E = exon and I = intron

5' UTR E₁ I₁ E₂ I₂ E₃ I₃ E₄ UTR 3'

Which components of the previous molecule will also be found in mRNA in the cytosol?

A. 5' UTR I₁ I₂ I₃ UTR 3'

B. 5' E₁ I₁ E₂ I₂ E₃ I₃ E₄ 3'

C. 5' UTR E₁ E₂ E₃ E₄ UTR 3'

D. 5' E₁ E₂ E₃ E₄ 3'

Locations of the processes involved in protein synthesis

In eukaryotic cells, the processes of protein synthesis occur in different cellular locations.

Drag the labels to the appropriate targets to identify where in the cell each process associated with protein synthesis takes place.

What occurs during some key processes of protein synthesis?

Match these key processes involved in protein synthesis to descriptions of what occurs at each step.

Where are cytoplasmic and secreted proteins made?

Both cytoplasmic and secreted proteins can only be synthesized in the presence of a ribosome. This diagram shows the two kinds of ribosomes:

• free ribosomes, which are found in the cytoplasm
• bound ribosomes, which are found on the membrane of the rough endoplasmic reticulum (ER)

Which statement correctly describes where cytoplasmic and secreted proteins are synthesized?

A. Both cytoplasmic and secreted proteins are synthesized on free ribosomes.

B. Cytoplasmic proteins are synthesized on free ribosomes, whereas secreted proteins are synthesized on ribosomes bound to the rough ER.

C. Cytoplasmic proteins are synthesized on ribosomes bound to the rough ER, whereas secreted proteins are synthesized on free ribosomes.

D. Both cytoplasmic and secreted proteins are synthesized on ribosomes bound to the rough ER.

Roles of RNA in protein synthesis in eukaryotes

RNA plays important roles in many cellular processes, particularly those associated with protein synthesis: transcription, RNA processing, and translation.

Drag the labels to the appropriate bins to identify the step in protein synthesis where each type of RNA first plays a role. If an RNA does not play a role in protein synthesis, drag it to the “not used in protein synthesis” bin.

The role of RNA primers

DNA synthesis (replication) and RNA synthesis differ in their needs for primer molecules.

• In DNA replication, DNA polymerase cannot initiate the formation of a new strand of DNA directly from DNA nucleotides alone. Instead, the process requires an RNA primer to which the nucleotides of the new DNA strand attach.
• In RNA synthesis, in contrast, RNA polymerase can initiate the formation of a new strand of RNA without any primers.

How do tRNA and rRNA function in protein synthesis?

Both tRNA (transfer RNA) and rRNA (ribosomal RNA) play essential roles in protein synthesis.

Which two statements correctly describe the roles of tRNA and rRNA in protein synthesis?

A. rRNA is the major structural component of ribosomes and is involved in binding both mRNA and tRNAs.

B. tRNAs implement the genetic code, translating information from a sequence of nucleotides to the sequence of amino acids that make up a protein.

C. tRNA transfers a nucleotide sequence from the DNA in the nucleus to the site of protein synthesis in the cytoplasm.

D. rRNA has many variations, each of which binds a specific amino acid.

What is the role of mRNA in protein synthesis?

mRNA (messenger RNA) plays a key role in protein synthesis as the intermediate between the information encoded by a sequence of bases in DNA (a gene) and the sequence of amino acids that make up the protein product.

Which three statements correctly describe the role that mRNA plays in protein synthesis in eukaryotes?

A. mRNA is produced only after the steps of RNA processing.

B. mRNA is the template for protein synthesis in translation.

C. mRNA links together amino acids, forming a polypeptide chain.

D. mRNA carries genetic information from the nucleus to the cytoplasm.

E. mRNA is the immediate product of transcription.

snRNAs and RNA processing

One stage of RNA processing in eukaryotes involves the removal of introns--non-coding regions interspersed within the coding regions of the pre-mRNA. In this RNA splicing process, the machinery that catalyzes the removal of introns (called the spliceosome) is composed of proteins and snRNAs (small nuclear RNAs).

The snRNAs (and associated proteins) have two functions in the splicing process:

• to bind to specific sequences of RNA that specify the location of the intron in the pre-mRNA, and
• to catalyze the splicing process itself.

Martian Part 1

Life as we know it depends on the genetic code: a set of codons, each made up of three bases in a DNA sequence and corresponding mRNA sequence, that specifies which of the 20 amino acids will be added to the protein during translation.

Imagine that a prokaryote-like organism has been discovered in the polar ice on Mars. Interestingly, these Martian organisms use the same DNA → RNA → protein system as life on Earth, except that

• there are only 2 bases (A and T) in the Martian DNA, and
• there are only 17 amino acids found in Martian proteins.

Based on this information, what is the minimum size of a codon for these hypothetical Martian life-forms?

A. 2 bases

B. 3 bases

C. 4 bases

D. 5 bases

E. 6 bases

F. The answer cannot be determined from the information provided.

Martian Part 2

A simple mathematical equation can correctly express the maximum number of codons that can be constructed from x different bases, with a codon length ofy bases. Recall that for life on Earth,

• there are 4 different bases (A, T, G, and C),
• a codon is 3 bases long, and
• there are a total of 64 possible codons that specify the 20 different amino acids (some amino acids are specified by more than one amino acid). This chart shows this redundancy in the genetic code for life on Earth.

Which of the following equations can be used to calculate the maximum number of codons (N) that can be constructed from x different bases when there are y bases per codon?

A. N = x ^y

B. N = x y

C. N = 4x(y+1)

D. N = 16(3x/y)

Martian Part 3

It is possible to solve this problem by simply listing all the possible combinations of bases for a given codon length. In the hypothetical Martian example there are only 2 different bases (A and T). For a codon length of 1 base, there would only be 2 possible codons:

A or T

For a codon length of 2 bases, there would be 4 possible codons:

AA; TA; AT; or TT

How many different Martian codons are possible if the codon length is 4 bases?

A. 12

B. 16

C. 32

D. 64

These three events happen every time mRNA is translated into a protein. Put these events in order. Rank from first to last. To rank items as equivalent, overlap them.

Translation Initiation Termination

What is the the function of tRNA?

A. It binds the mRNA before the ribosome and guides the ribosome to the correct binding place.

B. It recognizes and binds a specific codon on mRNA during translation.

C. It contains the gene coding for a protein.

What is the function of aminoacyl-tRNA synthetase?

A. It synthesizes amino acids.

B. It attaches amino acids to a specific tRNA.

C. It synthesizes tRNA.

Complete the following vocabulary exercise related to the process of translation of mRNA to protein by the ribosome.

Match the words in the left-hand column with the appropriate blank in the sentences in the right-hand column.

A particular triplet of bases in the coding sequence of DNA is AAA. The anticodon on the tRNA that binds the mRNA codon is _____.

A. UUA

B. UUU

C. AAA

D. TTT

Accuracy in the translation of mRNA into the primary structure of a polypeptide depends on specificity in the _____.

A. binding of the anticodon to small subunit of the ribosome

B. binding of ribosomes to mRNA

C. attachment of amino acids to rRNAs

D. binding of the anticodon to the codon and the attachment of amino acids to tRNAs

The release factor (RF) _____.

A. supplies a source of energy for termination of translation

B. releases the ribosome from the ER to allow polypeptides into the cytosol

C. binds to the stop codon in the A site in place of a tRNA

D. releases the amino acid from its tRNA to allow the amino acid to form a peptide bond

What type of bonding is responsible for maintaining the shape of the tRNA molecule shown in the figure above?

A. hydrogen bonding between base pairs

B. ionic bonding between phosphates

C. peptide bonding between amino acids

D. van der Waals interactions between hydrogen atoms

The tRNA shown in the figure above has its 3' end projecting beyond its 5' end. What will occur at this 3' end?

A. The amino acid binds covalently.

B. The excess nucleotides (ACCA) will be cleaved off at the ribosome.

C. The 5' cap of the mRNA will become covalently bound.

D. The small and large subunits of the ribosome will attach to it.

During elongation, which site in the ribosome represents the location where a codon is being read?

A. E site

B. A site

C. P site

D. the small ribosomal subunit

How does termination of translation take place?

A. The end of the mRNA molecule is reached.

B. A stop codon is reached.

C. The 5' cap is reached.

D. The poly-A tail is reached.

Which of the following types of mutation, resulting in an error in the mRNA just after the AUG start of translation, is likely to have the most serious effect on the polypeptide product?

A. a substitution of the third nucleotide in an ACC codon

B. a deletion of two nucleotides

C. a substitution of the first nucleotide of a GGG codon

D. a deletion of a codon

What does a mutagen cause?

Consider the meaning of the prefix "muta-."

A. decreased enzyme activity throughout the cell

B. a change in the sequence of DNA

C. decreased permeability of the nuclear envelope

D. problems with mitosis

E. a reduction in the number of tRNA molecules available for protein synthesis

A nonsense mutation in a gene _____.

A. changes an amino acid in the encoded protein

B. has no effect on the amino acid sequence of the encoded protein

C. introduces a premature stop codon into the mRNA

D. alters the reading frame of the mRNA

The most commonly occurring mutation in people with cystic fibrosis is a deletion of a single codon. This results in _____.

A. a polypeptide missing an amino acid

B. a frameshift mutation

C. a base-pair substitution

D. a nonsense mutation

How might a single base substitution in the sequence of a gene affect the amino acid sequence of a protein encoded by the gene, and why?

A. Only a single amino acid could change, because the reading frame is unaffected.

B. The amino acid sequence would be substantially altered, because the reading frame would change with a single base substitution.

C. All amino acids following the substitution would be affected, because the reading frame would be shifted.

D. It is not possible for a single base substitution to affect protein structure, because each codon is three bases long.