Fundamentals of Microbiology: Micro - Exam 2 Flashcards


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Ms. Richardson's Microbiology Class - Exam 2 Study cards
updated 9 years ago by Meagan_Gregory
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microbiology, microbial growth & nutrition, medical, infectious diseases, immunology, science, life sciences
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1

What is the asexual process by which a cell divides to form two new cells?

Binary Fission

2

The proper term for CYTOPLASMIC division is.....

Cytokinesis

3

Referring to an organism that grows in the presence OR absence of oxygen gas.

Facultative

4

Referring to an organism that does not require free oxygen.

Anaerobe

5

A lab test in which a statistical evaluation is used to estimate the number of bacteria in a sample of fluid; often employed in determinations of coliform bacteria in water.

Most Probably Number (MPN)

6

Each bacterial cell on an agar growth medium that divides to produce a discrete colony.

Colony Forming Unit (CFU)

7

The time interval between bacterial divisions.

Generation Time

8

An organic substance that helps stabilize the proteins in a bacterial spore, therefore increasing spore resistance.

Dipicolinic Acid

9

The cloudiness of a broth culture due to a bacterial growth.

Turbidity

10

The final portion of a bacterial growth curve in which environmental factors overwhelm the population and induce death; also called death phase.

Decline Phase

11

The portion of a bacterial growth curve in which the reproductive and death rates of cells are equal.

Stationary Phase

12

The portion of a bacterial growth curve during which active growth leads to a rapid rise in cell numbers.

Llogarithmic Phase or Log Phase

13

Referring to a prokaryotic cell requiring low oxygen gas and a high concentration of carbon dioxide gas for metabolism.

Capnophilic

14

A measure of the viable cells by counting the number of colonies on a plate; each colony presumably started from one viable cell.

Colony Forming Unit

15

A prokaryote living under extremely high temperature environments.

Hyperthermophile

16

The initial portion of a bacterial growth curve during which time the cells are preparing for reproduction (binary fission).

Lag Phase

17

A bacterium not inhibited by oxygen gas.

Aerotolerant

18

An accumulation or colony of microorganisms of one species.

Pure Culture

19

A chemically undefined medium in which the nature and quantity of each component has not been identified.

Complex Medium

20

A growth medium in which special nutrients are added to get a species to grow.

Enriched Medium

21

A microorganism that grows at acidic pH's below 4.

Acidophile

22

A procedure to estimate the number of cells in a sample dilution spread on an agar plate.

Standard plate count

23

A growth medium in which different species of microorganisms can be distinguished visually.

Differential medium

24

An organism that live in cold temperature ranges from 0-20 Celsius.

Psychrophiles

25

Referring to microorganisms that grow at 0*C but have a temperature optima of 20*C-40*C.

Psychrotolarent

26

Estimation of the number of cells by observation with the light microscope.

Direct microscopic count.

27

An extremely resistant dormant cell produced by some gram positive bacterial species.

Endospores

28

A process by which a mixed culture can be separated into pure colonies and the colonies isolated.

Pour plate method

29

Referring to an organism that grows best in an oxygen reduced environment.

Microaerophile

30

An archaeal organism living in an extreme environments.

Extremophile

31

A bacterial medium that binds oxygen gas from the atmosphere and creates an environment suitable for anaerobic growth.

Thioglycollate broth

32

A chemically defined medium in which the nature and quantity of each component is identified.

Synthetic medium

33

An organism that grows only in the absence of oxygen gas.

Obligate Anaerobe

34

A polysaccharide derived from marine seaweed that is used as a solidifying agent in many microbiological culture media

Agar

35

A common bacteriological growth medium consisting of beef extract, peptone, water and agar.

Nutrient Agar

36

An archaeal organism living in an extremely acidic environment.

Extreme halophile

37

Referring to prokaryotes that are viable but not culturable.

VBNC

38

An organism living in environments with high concentrations of salt.

Halophile

39

A process by which a mixed culture can be streaked onto an agar plate and pure colonies isolated.

Streak Plate Method

40

A growth medium that contains ingredients to inhibit certain microorganisms while encouraging the growth of others.

Selective medium

41

A microorganism that lives under conditions of high atmospheric pressure.

Barophile

42

The second portion of a bacterial growth curve, in which active growth leads to a rapid rise in cell numbers.

Llogarithmic or Log phase

43

A microbe that grows best without salt but can tolerate low concentrations.

Halotolerant

44

The process of spore formation.

Sporulation

45

An organism that does not require or cannot use oxygen gas for metabolism.

Anaerobe

46

An organism that cannot use oxygen gas for metabolism.

Obligate anaerobe

47

The time duration for a cell to double in number.

Generation time

48

A growth medium containing special nutrients used to grow fastidious organisms.

Enriched medium

49

A differential growth medium that encourages the growth of staphylococci by containing a high percentage of salt, which is inhibitory to most other microorganisms.

Mannitol Salt Agar (MSA)

50

A prokaryote that has an optimal growth temperature above 80*C.

Hyperthermophile

51

An organism that grows best in an oxygen reduced environment.

Microanaerobe

52

A microorganism that cannot grow in the presence of added sodium chloride

Nonhalophile

53

An organism that requires oxygen gas for metabolism.

Obligate Aerobe

54

An organism that lives in high temperature ranges of 40*C-90*C.

Thermophile

55

A chemically defined medium in which the components are not known.

Complex medium

56

The form of reproduction that maintains genetic constancy while increasing cell number.

Asexual reproduction

57

A bacterium not inhibited by oxygen gas

Aerotolerant

58

An organism that that grows in temperature ranges of 20*C-40*C.

Mesophiles

59

The production of ATP in the presence of an inorganic final electron acceptor other than oxygen gas.

Anaerobic respiration

60

The slowing down or prevention of a metabolic pathway when excess end product binds noncompetitively to an enzyme in the pathway.

Feedback inhibition

61

A cyclic series of enzyme-catalyzed reactions in which carbon Acetyl-CoA is released as carbon dioxide; the reactions also yield protons and high-energy electrons that are transported among coenzymes and cytochromes as their energy is released.

Krebs cycle

62

A biochemical process in which light energy is converted to chemical energy and used in carbohydrate synthesis.

Photosynthesis

63

A biochemical process in which amino groups are enzymatically removed from amino acids or other organic compound.

Deamination

64

An anabolic, energy-requiring process.

Endergonic reaction

65

An organism that uses light energy to synthesize nutrients from carbon compounds other than carbon dioxide.

Photoheterotroph

66

An organic molecule that forms the nonprotein part of an enzyme molecule

Coenzyme

67

A metal ion that is needed for enzyme function.

Cofactor

68

An organism that derives energy from organic chemicals and uses the energy to synthesize carbon compounds other than carbon dioxide.

Chemoheterotroph

69

The substance(s) in which an enzyme acts.

Substrate

70

The sum of all biochemical processes taking place in a living cell.

Metabolism

71

The region of the enzyme where the substrate binds.

Active site

72

A pigmented molecule that functions in photosynthesis; exists free in the cytoplasm of prokaryotes and within the chloroplasts of eukaryotes.

Chlorophyll

73

The production of ATP using the organic compound Pyruvate as the final electron receptor.

Fermentation

74

The use of a proton gradient across a membrane to generate cellular energy in the form of ATP.

Chemiosmosis

75

A six-carbon sugar used as a major energy source for metabolism.

Glucose

76

A type of heterotrophic organism that feeds on dead organic matter, such as rotting wood or compost.

Saprobe

77

A pigment located in the membrane systems of purple sulfur bacteria that upon excitement by light, loses electrons and initiates photosynthetic reactions.

Bacteriochlorophyll

78

The inhibition of enzyme function through the binding of a molecule to a non-active site on the enzyme.

Noncompetitive Inhibition

79

A chemical process releasing energy

Exergonic

80

A series of enzyme-catalyzed chemical reactions in which glucose is broken down into two molecules of pyruvate with a net gain of 2 ATP molecules.

Glycolysis

81

The gain of electrons by a molecule.

Reduction

82

A chemical change in which electrons are lost by an atom.

Oxidation

83

A type of heterotrophic organism that feeds on live organic matter such as another organism.

Parasite

84

An organism that uses light energy to synthesize nutrients from carbon dioxide.

Photoautotroph

85

A form of photosynthesis in which molecular oxygen is produced.

Oxygenic Photosynthesis

86

A group of pigments that act as a light trapping system for photosynthesis

Photosystem

87

A substance(s) resulting from a chemical reaction

Product