The initial work on anaphylaxis was done by
Watson and Crick.
Pasteur.
Fleming.
Richet and Portier.
Richet and Portier
If the immune system responds inadequately to antigenic stimulation, this is termed
hypersensitivity.
autoimmunity.
cell-mediated immunity.
immunodeficiency.
immunodeficiency
The immunoglobulin associated with Type I hypersensitivity is
IgG.
IgA.
IgM.
IgE.
IgD.
IgE
IgE molecules involved in hypersensitivity reactions have become attached to
neutrophils.
mast cells.
B
cells.
macrophages.
mast cells AND B cells.
mast cells
To produce an allergic reaction in Type I hypersensitivity, the antigen
must bind to mast cells.
must bind to free IgE
molecules.
must bind to IgE on mast cells.
must crosslink
two IgE molecules on mast cells.
Must crosslink two IgE molecules on mast cells
During a Type I hypersensitivity reaction, the mast cells
become phagocytic.
release IgE
antibodies.
degranulate.
immediately release
histamine.
degranulate AND immediately release histamine.
degranulate and immediately release histamines
Urticaria is characterized by
living in Utica.
wheal and flare.
asthma.
inflammation.
Wheal and flare
Generalized anaphylaxis is generally characterized by
wheal and flare.
inflammation.
shock.
rash.
Shock
Most cases of generalized anaphylaxis are a result of
fire ant stings.
aspirin.
bananas.
peanuts, bee
stings or penicillin injections.
peanuts, bee stings, or penicillin injections
Desensitization
stimulates an increase in IgG.
reduces the number of mast
cells.
increases the number of basophil cells.
is a
treatment for hypersensitivity reactions.
stimulates an increase
in IgG AND is a treatment for hypersensitivity reactions.
Stimulates an increase in IgG and is a treatment for hypersensitivity reactions
The hypersensitivity treatment that stimulates an increase of IgG and T suppressor cells and a decrease in IgE is known as
desensitization.
immunity.
sensitization.
exposure.
anaphylaxis.
Desensitization
Recombinant human monoclonal antibody (rhuMAb)
appears promising as a treatment for asthma.
decreases the
levels of IgG.
uses an engineered form of an IgG
molecule.
promotes crosslinking between IgE molecules on the mast
cells.
appears promising as a treatment for asthma AND uses an
engineered form of an IgG molecule.
Appeas promising as a treatment for asthma and uses an engineered form of an IgG molecule
The type of hypersensitivity expressed with the lysing of red blood cells is
Type I.
Type II.
Type III.
Type IV.
Type V.
Type II
A transfusion reaction primarily involves
leukocytes.
phagocytes.
platelets.
erythrocytes.
erythrocytes
The natural antibodies in serum that react with A or B polysaccharide antigens are mostly of the class
IgG.
IgE.
IgM.
IgD.
IgA.
IgM
Anti-A and anti-B antibodies
are considered natural antibodies.
are present at
birth.
are typically IgM.
easily cross the
placenta.
are considered natural antibodies AND are typically IgM.
Are considered natural antibodies and are typically IgM
Regarding a mismatch of either the Rh antigen or the AB antigen, both
result in destruction of red blood cells.
utilize complement
to destroy red blood cells.
utilize antibody-dependent cellular
cytotoxicity to destroy the red blood cells.
result in
destruction of only leukocytes.
result in destruction of red blood cells
Antibodies that have arisen in the blood plasma without any obvious or deliberate stimulus are called
natural.
acquired.
injurious.
active.
Natural
Hemolytic disease of the newborn
may not manifest itself fully until after birth.
is due to
the action of IgM.
is due to the action of IgE.
is a Type I
hypersensitivity reaction.
may not manifest itself fully until
after birth AND is due to the action of IgE.
May not manifest itself fully until after birth
The cell type responsible for Type II hypersensitivity is the
mast cell.
B cell.
macrophage.
platelet.
neutrophils.
B cell
Immune complexes
consist of antigen-antibody bound together.
are usually
cleared rapidly from the body.
bind to Fc receptors on
cells.
are involved in Type III hypersensitivity
reactions.
All of the choices are correct.
All of the choices are correct
Localized injury or death of tissue resulting from repeated injections of an antigen into a person with high levels of circulating specific antibody is known as
farmer's lung.
German measles.
serum sickness.
an
Arthus reaction.
An Arthus reaction
Arthus reactions and serum sickness are examples of _________ hypersensitivity.
type I
type II
type III
type IV
type V
type III
Which of the following disease states is not among those caused by immune complexes?
farmer's lung
serum sickness
hay fever
glomerulonephritis
Hay fever
Delayed hypersensitivity is also known as ___________ hypersensitivity.
type I
type II
type III
type IV
type V
Type Iv
Delayed type hypersensitivity primarily involves
erythrocytes.
B cells.
T cells.
mast cells.
T cells
Type IV hypersensitivity reactions typically peak within
minutes.
hours.
12 hours.
2-3 days.
2-3 days
Which of the following is associated with contact dermatitis?
poison ivy
latex
tuberculin skin test
hay
fever
poison ivy, latex AND tuberculin skin test
Posion ivy, latex and tuberculin skin test
The redness and induration found after a tuberculin skin test
involve the action of
sensitized T
cells.
IgE.
complement.
basophil cells.
Sensitized T cells
Patch tests are used to detect
hives.
serum sickness.
immune complexes.
contact hypersensitivity.
Contact hypersensitivity
Which of the following have been an effective immunosuppressant for use in transplantation?
amphotericin B and cyclosporin A
FK506 and
cephalosporin
cyclosporin A and tacrolimus
cephalosporin and
amphotericin B
FK506 and amphotericin B
cyclosporin A and tacrolimus
Graft-versus-host disease is primarily a
Type I reaction.
Type II reaction.
Type III
reaction.
Type IV reaction.
Type V reaction.
Type IV reaction
The tissue antigens most involved in graft rejection involve
Rh.
ABO.
MHC.
MLB.
MHC
Killing of graft cells occurs through a complex series of mechanisms including
sensitized T cytotoxic cells.
NK cells.
erythrocytic
cells.
basophilic cells.
sensitized T cytotoxic cells AND NK cells.
sensitized Tcytotoxic cells and NK cells
Cyclosporin A
is a relatively general immunosuppressive agent.
suppresses T
cell proliferation.
activates macrophages.
stimulates
antibody production.
is a relatively general immunosuppressive
agent AND suppresses T cell proliferation.
Supresses T cell proliferation
Allografts
are normally rejected within hours.
are normally rejected
within 10-14 days.
are grafts between non-identical members of
the same species.
would include the fetus.
are normally
rejected within 10-14 days, are grafts between non-identical members
of the same species AND would include the fetus.
Are normally rejected within 10-14 days, are grafts between non-identical members of the same species AND would include the fetus
Immunologically privileged sites include the
brain.
eyes.
testes.
kidney.
brain, eyes AND testes.
brain, eyes, and testes
The fetus is not rejected because
it is too small.
it is in an immunologically privileged
site.
the father is immunosuppressed.
it has no
antigens.
it is in an immunologically privileged site AND the
father is immunosuppressed.
It is an immunologically privileged site
Which of the following primary immunodeficiencies is the most common?
severe combined immunodeficiency
selective IgA
deficiency
agammaglobulinemia
Di George's syndrome
Selectove IgA deficiency
If the thymus fails to develop
functional T cells are absent.
functional B cells are
absent.
Di George's syndrome exists.
complement deficiencies
exist.
functional T cells are absent AND Di George's syndrome exists.
Functional T cells are absent and Di George's syndome exists
If a patient lacks B cells, the resulting disease is
SCID.
AIDS.
Di George's syndrome.
agammaglobulinemia.
Agammaglobulinemia
Defects in bone marrow stem cells result in a condition known as
SCID.
AIDS.
Di George's syndrome.
Chediak-Higashi disease.
SCID
A defect in which of the following systems leads to granulomatous disease?
lymphatic system
circulatory system
oxidase
system
Golgi system
Oxidase system
The condition that results from ineffective digestion after phagocytosis is
AIDS.
Chediak-Higashi disease.
Di George's syndrome.
agammaglobulinemia.
Chediak-Higashi disease
Secondary immunodeficiency disease is not the result of
genetic defects.
malignancies.
advanced age.
malnutrition.
genetic defects
HIV
appears to cause AIDS.
is an RNA virus.
destroys T
helper cells.
makes the patient vulnerable to infections,
especially those caused by opportunists.
All of the choices are correct.
All choices are correct
If the body recognizes parts of itself as being foreign, this is termed
immunodeficiency disease.
agammaglobulinemia.
autoimmune disease.
AIDS.
autoimmune disease
Myasthenia gravis is an example of an autoimmune disease that involves
sensitized T cells.
cytotoxic T cells.
antibodies.
IgD.
Antibodies
Insulin-dependent diabetes mellitus is an example of an
autoimmune disease that involves
sensitized T
cells.
cytotoxic T cells.
antibodies.
IgD.
cytotoxic T cells
Stem cells
have an almost unlimited capacity to divide.
can
differentiate into different tissues.
may be used to test the
effects of drugs on human cells.
come from fetal
material.
All of the above
All of the above
First exposure to an allergen results in a violent hypersensitivity reaction.
True
False
False
Anaphylaxis is the name given to allergic reactions caused by IgE-mediated release of mast cell granules.
True
False
True
Generalized anaphylaxis may be quickly controlled with the use of antihistamines.
True
False
False
Allergic rhinitis and hives may both respond to antihistamines.
True
False
True
Type O blood is missing both anti-A and anti-B antibodies.
True
False
True
Anti-A and anti-B antibodies are natural antibodies and are present at birth.
True
False
False
Mismatch of either the Rh antigen or the AB antigen results in lysis of red blood cells by complement.
True
False
False
Anti-Rh antibodies may not cross the placenta.
True
False
False
MHC plays a pivotal role in transplant rejection.
True
False
True
A lack of T cells makes one more vulnerable to intracellular parasites.
True
False
True
The idea that communicable diseases were caused by the passage of living things from one person to another was first put forth by
Fracastorius.
Pasteur.
Thucydides.
Leeuwenhoek.
Fracastorius
The connection between a particular organism and a specific disease
was first made by
Fracastorius.
Pasteur.
Koch.
Leeuwenhoek.
Koch
The series of steps used to connect an organism to a disease are known as
Pasteur's postulates.
Lister's aseptics.
Linnaeus
taxonomics.
Koch's postulates.
Koch's postulates
The interaction of all organisms within a biological community is called a(n)
dialogue.
chat room.
ecosystem.
relationship.
Ecosystem
The microorganisms that are regularly found in or on the body, yet do no apparent harm are called
abnormal flora.
transient flora.
variant
flora.
normal flora.
Normal flora
The microorganisms that are occasionally found in or on the body are called
abnormal flora.
transient flora.
variant
flora.
normal flora.
Transient flora
Organisms that are found together and interact on a more or less permanent basis are in a relationship termed
mutualism.
parasitism.
symbiosis.
transient flora.
symbiosis
The symbiotic relationship wherein both partners benefit is termed
commensalism.
parasitism.
independence.
mutualism.
Mutualism
A relationship in which one partner benefits and the other is unaffected is termed
commensalism.
parasitism.
independence.
mutualism.
commensalism
A relationship in which one partner benefits and the other is harmed is termed
commensalism.
parasitism.
independence.
mutualism.
parasitism
The resident microbial population of the human fetus is
zero.
sparse.
complex.
symbiotic.
Zero
Which of the following is true about the role normal flora plays in maintaining host health?
They provide a surface that is incompatible for attachment of an
invader.
They establish competition for nutrients and
vitamins.
They produce antimicrobial substances.
They
stimulate the immune system.
All of the choices are true.
All choices are true
Which of the following members of the normal flora inhibit the growth of Candida albicans?
E. coli.
Lactobacillus species.
Staphylococci
species.
Propionibacterium species.
Lactobacillus species
The composition of the normal flora may be affected by
hormonal changes.
use of antibiotics.
obesity
level.
diet.
All of the choices are correct.
All of the answers are correct
The "hygiene hypothesis" proposes that
lack of exposure to microbes can promote development of
allergies.
cleanliness truly is next to godliness.
hand
washing is the best preventative measure against infection.
the
immune system develops best in a clean environment.
Lack of exposure to microbes can promote develpment of allergies
The infectious dose
is the same for all microorganisms.
may be 10-100 cells for
Salmonella.
is expressed as ID50.
is defined as the number
of microbes necessary to ensure infection.
is expressed as ID50
AND is defined as the number of microbes necessary to ensure infection.
Is expressed as ID50 AND is defined as the number of microbes necessary to ensure infection
The number of organisms necessary to insure infection is termed the
infectious dose.
fatal number.
minimum lethal
dose.
pathogenic number.
Infectious dose
A disease-causing microorganism or virus is referred to as a(n)
avirulent infection.
colony.
commensal.
pathogen.
Pathogen
Opportunists or opportunistic pathogens
are usually saprophytes.
take advantage of special
circumstances.
are usually mutualistic.
always cause disease.
Take advantage of special circumstances
The suffix -emia means in the
body.
lymph.
interstitial tissue.
blood.
Blood
Attributes
of an organism that promote pathogenicity are called
disease
factors.
colonization factors.
mutualistic.
virulence factors.
Virulence factors
Avirulent organisms are
more likely to cause disease.
more likely to cause severe
disease.
unable to cause disease.
pathogenic.
Unable to cause disease
Which of the following may be considered virulence factor(s)?
adhesins
capsules
endotoxins
proteases
All of
the choices are correct.
All of the choices are correct
Which of the following does S. pneumoniae use to survive in the host?
plasmids
pili
flagella
capsules
capsules
Which of the following would be considered a sign of a disease?
headache
pain
nausea
fever of 39°C
Fever at 39 degrees C
People who carry and may spread pathogenic organisms without any overt symptoms of illness are called
primary infections.
secondary infections.
mutualists.
carriers.
carriers
The spread of toxin via circulation is called
septicemia.
bacteremia.
sepsis.
toxemia.
toxemia
A more modern equivalent to Koch's Postulates is termed
Pasteur's Systematics.
Hoch's Postulates.
Atomic
Theory.
Protein Theory.
Molecular Postulates.
Molecular Postulates
Species of both Shigella and Streptococcus
invade host cells.
produce a toxin.
cause ergot
poisoning.
are delivered via flea bites.
invade host cells
AND produce a toxin.
invade host cells AND produce a toxin
Which of the following causes a foodborne intoxication?
Staphylococcus aureus
E. coli O157:H7
Clostridium
botulinum
Mycobacterium tuberculosis
Staphylococcus aureus,
E. coli O157:H7 AND Clostridium botulinum
Stapholococcus aureus, E. coli, O157:H7 and clostridium
Adhesins are
involved in the first step of the infectious process.
often
found at the tip of pili.
found in
flagella.
endotoxins.
involved in the first step of the
infectious process AND often found at the tip of pili.
involved in the first step of the infecious process and found at the tip of the pili
The first step in the establishment of infection is that the organism must
invade host tissues.
attach to host cells.
evade
phagocytes.
produce toxins.
attach to host cells
Which of the following factors is not considered important for the establishment of an infection?
adherence
dose
toxicity
virulence factors
toxicity
Typically, adhesins
are found on pili.
help bacteria attach to host
cells.
are proteins.
are found on host cells.
are
found on pili, help bacteria attach to host cells AND are proteins.
Are found on pili, help bacteria attach to hosts and are proteins
The lack of susceptibility to diseases of other species in humans may be due to the
secretion of exotoxins.
presence of endotoxins.
action of
IL-2.
lack of receptors that are recognized by adherence factors.
lack of receptors that are recognized by adherence factos
An example of genetic variation used in pathogen survival may be
production of a comet's tail.
protease
production.
inhibition of MHC Class I antigen
production.
changing the pilus type.
production of a comet's
tail AND protease production.
changing the pilus type
Colonization of the body is inhibited by
the shedding of skin cells.
the movement of mucus by
cilia.
peristalsis.
the flushing action of the urinary
tract.
All of the choices are correct.
All of the choices are correct
The process by which infectious agents are ingested by host cells is termed
exocytosis.
pinocytosis.
endocytosis.
phagosome fusion.
endocytosis
Bacteria that resist killing by complement proteins are termed
carriers.
serum resistant.
balanced
pathogens.
mutualistic.
carriers AND serum resistant.
serum resistant
C5a peptidase
is a virulence factor.
synthesizes C5a.
is produced by
the host cell in response to infection.
is a molecule promoting
chemotaxis.
is a virulence factor AND is a molecule promoting chemotaxis.
is a virulence factor
Bacteria may survive phagocytosis by
preventing fusion of the lysosome with the phagosome.
lysing
the phagosome.
producing comet tails.
preventing fusion of
two phagosomes.
preventing fusion of the lysosome with the
phagosome AND lysing the phagosome.
preventing fusion of the lysosome with the phagosome and lysing the phagosome
The chemical nature of endotoxins is that of a
protein.
nucleic acid.
lipid.
lipopolysaccharide.
lipopolysaccharide
The chemical nature of exotoxins is that of a
protein.
carbohydrate.
lipid.
lipopolysaccharide.
protein
Which is true about superantigens?
They are a type of exotoxin.
They bind to MHC class II antigen
on T cells.
They enhance specific antibody production.
They
are processed intracellularly.
They are a type of exotoxin AND
they bind to MHC class II antigen on T cells.
They are a type of exotoxin and they bind to MHC class II antigen on T cells
Which is true about botox?
It is an endotoxin.
It is produced by S. aureus.
It may
cause botulism.
It is useful in treating conditions related to
muscle contractions.
It may cause botulism AND it is useful in
treating conditions related to muscle contractions.
It may cause botulism and it is useful in treating conditions related to muscle contractions
Which of the following is/are true about endotoxins?
Lipid A is the toxic portion of the molecule.
The toxic
effects depend on the bacteria from which it came.
The lipid A
is immunogenic.
They are proteins.
The toxic effects depend
on the bacteria from which it came AND they are proteins.
Lipid A is the toxic portion of the molecule
Which is/are true of viruses?
They may suppress the production of MHC Class I protein.
They
may produce an MHC Class I mimic protein.
They may prevent cell
suicide.
They may bind to MHC class II antigens.
They may
suppress the production of MHC Class I protein, they may produce an
MHC Class I mimic protein AND they may prevent cell suicide
They may supress the production of MHC class I protein, they may produce an MHC class I mimic protein AND they may prevent cell suicide
Disease(s) in which the causative agent becomes latent is/are
cold sores.
genital
herpes.
typhus.
shingles.
All of the choices are correct.
All of the choices are correct
The damage caused by parasites may be due to
competition for nutrients.
the physical blocking of
organs.
the direct digestion of host tissue.
the host's
immune response.
All of the choices are correct.
All of the choices are correct
The most successful parasites are the ones that live in harmony with their hosts.
True
False
True
A human fetus has no resident microbial population.
True
False
True
Infection always leads to disease.
True
False
False
A disease is an infection that impairs the normal state of health.
True
False
True
Obligate intracellular parasites may be grown in special synthetic media.
True
False
False
During incubation and convalescence a person may still spread infectious organisms.
True
False
True
The infectious dose of most pathogens is about equal.
True
False
False
A strong attachment of a microorganism to a host cell automatically leads to disease.
True
False
False
High concentrations of some bacteria are necessary for successful invasion because only at high density are their virulence genes expressed.
True
False
True
Only Gram-positive bacteria produce exotoxins.
True
False
False
One of the earliest researchers to explore the use of chemicals to kill microbial pathogens was
Koch.
Hooke.
Fleming.
Ehrlich.
Ehrlich
The arsenic compound that proved highly effective in treating syphilis was called
penicillin.
sulfa.
erythromycin.
Salvarsan.
Salvarsan
The first example of an antimicrobial drug synthesized in the laboratory was
penicillin.
sulfa.
erythromycin.
Salvarsan.
Salvarsan
Prontosil effectively acted on streptococci when the drug was split by enzymes to produce
penicillin.
sulfanilamide.
erythromycin.
Salvarsan.
Sulfanilamide
The use of Salvarsan and Prontosil to treat microbial infections were early examples of
antibiotics.
toxins.
inhibitors.
chemotherapy.
Chemotherapy
Penicillin was discovered by
Koch.
Hooke.
Fleming.
Ehrlich.
Fleming
The most effective form of penicillin is
A.
B.
E.
G.
G
One of the earliest antimicrobials isolated from a bacterium was
penicillin.
ampicillin.
Salvarsan.
streptomycin.
Streptomycin
Which of the following groups of microorganisms produces antibiotics?
Penicillium
Streptomyces
Bacillus
All of the choices
are correct.
Penicillium AND Streptomyces
All of the choices are correct
An antibiotic made by microorganisms and modified by chemists is called
anti-metabolic.
catabolic.
synthetic.
semi-synthetic.
Semi synthetic
The antimicrobials produced by some molds and bacteria are generally called
insecticides.
biocides.
antiseptics.
antibiotics.
antibiotics
The toxicity of a given drug is expressed as the
selective toxicity.
biocide index.
biostatic
index.
therapeutic index.
Therapeutic index
A high therapeutic index is
more toxic to the patient.
less toxic to the patient.
has
no effect on the patient.
has no effect on the pathogen.
Less toxic to the patient
Drugs that are bacteriostatic
kill bacteria.
promote bacterial growth.
inactivate
bacterial spores.
inhibit the growth of bacteria.
inhibit the growth of bacteria
Antimicrobials that kill microorganisms have the suffix
-cidal.
-static.
-anti.
-genic.
-cidal
Antimicrobials that inhibit the growth of microorganisms have the suffix
-cidal.
-static.
-anti.
-genic.
Static
Antibiotics that affect various strains of Gram-positive bacteria and various strains of Gram-negative bacteria are called
isolate usable.
stress-induced.
narrow-spectrum.
broad-spectrum.
Broad spectrum
The rate of elimination of an antimicrobial is expressed as its
metabolic destructive rate.
half-life.
effective
time.
dosage rate.
half life
Antibiotics that are most likely to disrupt the normal flora are termed
narrow-spectrum.
broad-spectrum.
targeted spectrum.
broad spectrum
Drugs that are more effective when taken together are called
energetic.
antagonistic.
subtractive.
synergistic.
synergistic
If drugs are less effective when taken together than when each is taken separately, they are called
energetic.
antagonistic.
additive.
synergistic.
antagonistic
Antimicrobials may produce
allergic reactions.
toxic effects.
suppression of normal
flora.
All of the choices are correct.
All of the choices are correct
Which of the following bacteria have an innate resistance to penicillin?
S. aureus
S. epidermidis
M. luteus
Mycoplasma
Myoplasma
Which of the following drugs target peptidoglycan?
penicillin
cephalosporin
vancomycin
bacitracin
All
of the choices are correct.
All of the choices are correct
All members of the penicillin family have
beta-lactam rings.
alpha-lactam rings.
phenolic
rings.
sulfanilic rings.
beta- lactam rings
Penicillin-binding proteins
primarily function in the cell to bind to beta-lactam
drugs.
are enzymes.
are involved in cell wall
synthesis.
inhibit non-growing bacteria.
are enzymes AND are
involved in cell wall synthesis.
Are enzymes and are involved in the cell wall synthesis
Beta-lactamases
bind to penicillin-binding proteins.
bind to
peptides.
prevent the linking of glycan chains in
peptidoglycan.
break the beta-lactam ring.
break the beta-lactam ring
The major class(es) of antibiotics that inhibit protein synthesis is/are
aminoglycosides.
tetracyclines.
macrolides.
bacitracins.
→
aminoglycosides, tetracyclines AND macrolides.
aminogylcosides, tetracyclines and macrolides
Inhibitors of protein synthesis typically key on
peptidoglycan precursors.
penicillin-binding
proteins.
ribosomes.
porin proteins.
ribosomes
Which is true of aminoglycosides?
They are bacteriostatic.
They irreversibly bind to the 30S
ribosomal subunit.
They block peptidoglycan synthesis.
They
are bactericidal.
They irreversibly bind to the 30S ribosomal
subunit AND they are bactericidal.
They irreversible bind the 30S ribosomal subunit and they are bactericidal
Fluoroquinolones typically target
ribosomes.
penicillin-binding
proteins.
peptidoglycan.
DNA gyrase.
DNA gyrase
Sulfonamide and trimethoprim are both
examples of metabolic inhibitors.
folate
inhibitors.
protein synthesis inhibitors.
inhibitors of cell
wall synthesis.
examples of metabolic inhibitors AND folate inhibitors.
exampes of metabolic inhibitors and folate inhibitors
Folic acid is ultimately used in the synthesis of
topoisomerases.
proteins.
DNA gyrases.
sulfonamides.
coenzymes.
coenzymes
Sulfonamides are similar in structure to
DNA gyrases.
LPS.
ribosomes.
PABA.
PABA
Sulfonamides work as
competitive inhibitors.
noncompetitive
inhibitors.
ribosome-binding molecules.
feedback inhibitors.
Competitive inhibitors
Trimethoprim and sulfonamides have a(n)
antagonistic effect.
synergistic effect.
energetic effect.
subtractive.
synergistic effect
Mycolic acids are targeted by isoniazid in the treatment of
S. aureus.
S. epidermidis.
M. luteus.
M. tuberculosis.
M tuberculosis
The lowest concentration of a drug that prevents growth of a microorganism is the
infectious dose.
lethal dose.
effective
dose.
minimum inhibitory concentration.
minimum inhibitory concentration
The minimum bactericidal concentration is the lowest concentration of a specific antimicrobial drug that kills _______ of a specific type of bacteria.
10%
50%
99.9%
100%
99.9%
The diffusion bioassay
determines the concentration of antimicrobial necessary to kill a
bacteria.
determines the concentration of antimicrobial necessary
to inhibit growth of a bacteria.
is similar in principal to the
Kirby-Bauer test.
determines the concentration of antimicrobial
in a fluid.
is similar in principal to the Kirby-Bauer test AND
determines the concentration of antimicrobial in a fluid.
Is similar in principal to the Kirby Bauer test and determines the concentration of antimicrobial in a fluid
Which test is used to determine the susceptibility of a microorganism to an antimicrobial?
MIC
MIB
MLB
Kirby-Bauer test
Kirby-Bauer test
The zone size obtained in the Kirby-Bauer test is influenced
by the
drug's
size.
stability.
concentration.
All of the
choices are correct.
All of the choices are correct
A commercial modification of the disk diffusion test is called the
A test.
B test.
C test.
D test.
E test.
E test
Bacteria may become antibiotic resistant due to
drug-inactivating enzymes.
alteration in the target
molecule.
decreased uptake of the drug.
increased
elimination of the drug.
All of the choices are correct.
All of the choices are correct
The most common method of transfer of antimicrobialresistance is through the use of
viruses.
R plasmids.
introns.
exons.
R plasmids
Compliance problems are leading to a large increase in antibiotic resistant strains of
Streptococcus.
Staphylococcus.
Mycobacterium.
Pseudomonas.
Mycobacterium
Antiviral drugs may target
uncoating.
nucleic acid synthesis.
viral
assembly.
viral ribosomes.
uncoating, nucleic acid synthesis
AND viral assembly.
Uncoating, nucleic acid synthesis, and viral assembly
The target of most antifungal drugs is
the
ribosome.
nucleus.
cholesterol.
ergosterol.
cholesterol
AND ergosterol.
ergosterol
The key characteristic of a useful antimicrobial is selective toxicity.
True
False
True
Antimicrobials that have a high therapeutic index are less toxic to the patient.
True
False
True
Broad-spectrum antibiotics have minimal effect on the normal flora.
True
False
False
Certain antimicrobials may be life-threatening.
True
False
True
Drugs that target peptidoglycan do not affect eukaryotes.
True
False
True
Beta-lactam drugs are only effective against growing bacteria.
True
False
True
The MBC may be determined by an extension of the MIC.
True
False
True
Antimicrobial resistance can be due to spontaneous mutation or gene acquisition.
True
False
True
Viruses are very effectively treated with antibiotics.
True
False
False
Antifungal drugs usually target the cell membrane.
True
False
True
Naturally acquired immunity
Acquisition of adaptive immunity through natural events
What does immunization do?
Mimics the events of naturally acquired immunity by inducing articially acquired immunity
What two subdivisions can natural or artifical immunity be divided into
Active immunity and passive immunity
Active immunity
Results from immune response upon exposure to an antigen and can occur either naturally following illness or artifically after immunization
Passive Immunity
Occurs naturally during pregnancy because the IgG from the mother cross the placenta
How does passive immunity occur naturally?
As a result of breast feeding. IgA antibodies in breast milk given to the child
What does artifical passive immunity involve?
The transfer of antibodies produced by another person or animal and can be usedto prevent disease before or after exposure
Attenuated vaccines
-Weakened form of pathogens and is generally unable to cause disease
What happens when the strain replicates in vaccine recipient (attenuated vaccines)
It causes an infection with undetectable or mild symptoms which results in long lasting immunity
Anthrax
-Acellular
-People in occupations that put them at risk such as military personnel
Advantages of attenuated vaccines
-Single dose is usually sufficient to induce long lasting immunity
-Vaccine has added potential for being spread
Disadvantages of attentuated vaccines
-Has potential to cause disease in immuncompromised individuals
-Pregnant women should avoid it
Attenuated vaccines in use include
-Sabin polio vaccine
-MMR
-Yellow fever
Inactivated vaccines
-Unable to replicate in vaccinated recipient
-Retains immunogenicity of infectious agent (not pathogenic)
What two categories do inactivated vaccines fall under?
-Whole agents and Fragments
Whole Agents (inactivated vaccines)
-Contain killed organisms of inactivated virus
-Does not change epitopes
-Cholera, plague, influenza, and Salk polio
Fragments (inactivated vaccines)
-Portions of organisms or agents including toxins, proteins, and cell wall components
-Includes toxoids, protein subunit vaccines and polysaccharide vaccines
Seronegative
Person not yet exposed to antigen and has no specific antibodies
Seropositive
Person with exposure and actively producing antibodies
Titer
Concentration of antibody in serum- indicates previous exposure
Serum
Fluid portion of the blood with no clotting factors
Plasma
Fluid portion of the blood with clotting factors
What is used to produce known antibodies?
Laboratory animals
How are animals used to produce known antibodies?
-Animal is immunized with antigen and produces specific antibodies
-Antibodies are retrieved by harvesting the animal's serum
Quantifying antigen-antibody reactions
-Concentrations of antibody are usually determined through dilution
-Antigen is added to dilution and titer is taken from last dilution to give detectable reaction
What do antigen-antibody complexes form?
Aggregates
Antigen-antibody binding can be seen in what kind of reactions
precipation and agglutination
Precipitation Reactions
-Antibodies binding to soluble antigen form insoluble complexes and precipiatete out of solution
How to achieve concentrations in precipiation reactions?
Place separate antigen and antibody suspensions side by side
Most widely known immunodiffusion test
ouchterlony
Antigen and antibody are placed in seperate wells cut in the gell and they diffuse and meet between the wells what does this form?
Line of precipiation at zone of optimal proportion
Radial immunodiffusion test
quantitative immodiffusion test
How does radial immunodiffusion test go?
-Antibody is added to liquid agar that is allowed to harden then antigen is added to the wells cut in the gel and diffuses outward forming a concentration gradient and a ring forms at antigen antibody precipiation
Immunoelectrophoresis
-Proteins seperated using gel electroporesis
-Antibodies are placed in wells and allowed to diffuse towards seperate proteins
-Line of precipation forms at antibody protein recognition
-Used to determind patient antibody leve;s
What can high levels of certain antibody classes indicate?
Disease
Aggulation reactions
-Large insoluble particles are involved
-Obvious aggregations are formed making them easier to see
Two types: direct and indirect
Direct agglutination
Specific antibody mixed with insoluble antigen and readily visible clumping indicates positive result
Indirect agglutination
Amplifies aggregation formation
-Antibody attaches to latex bead
-Agglutination of these beads is much easier to see
Using labeled antibodies to detect interactions
-Detectable markers can be attached to specfic antibodies which are marked to detect presence of given antigen
Which tests are included in labeled antibody detection?
-Fluorescent Antibody test(FA)
-Enzyme linked immunosorbant assay(ELISA)
-Western blotting
-Flourescence activates cell sorter )FACS)