A particular triplet of bases in the template strand of DNA is 5' AGT
3'. The corresponding codon for the mRNA transcribed is
A) 3'
UCA 5'.
B) 3' UGA 5'.
C) 5' TCA 3'.
D) 3' ACU 5'.
E) either UCA or TCA, depending on wobble in the first base.
A
The genetic code is essentially the same for all organisms. From
this, one can logically assume which of the following?
A) A gene
from an organism can theoretically be expressed by any other organism.
B) All organisms have experienced convergent evolution.
C)
DNA was the first genetic material.
D) The same codons in
different organisms translate into the different amino acids.
E)
Different organisms have different numbers of different types of amino acids
A
Which of the following nucleotide triplets best represents a codon?
A) a triplet separated spatially from other triplets
B) a
triplet that has no corresponding amino acid
C) a triplet at the
opposite end of tRNA from the attachment site of the amino acid
D) a triplet in the same reading frame as an upstream AUG
E) a sequence in tRNA at the 3' end
D
Which of the following provides some evidence that RNA probably
evolved before DNA?
A) RNA polymerase uses DNA as a template.
B) RNA polymerase makes a single-stranded molecule.
C) RNA
polymerase does not require localized unwinding of the DNA.
D)
DNA polymerase uses primer, usually made of RNA.
E) DNA
polymerase has proofreading function.
D
Which of the following does not occur in prokaryotic eukaryotic gene
expression, but does in eukaryotic gene expression?
A) mRNA,
tRNA, and rRNA are transcribed.
B) RNA polymerase binds to the
promoter.
C) A poly-A tail is added to the 3' end of an mRNA and
a cap is added to the 5' end.
D) Transcription can begin as soon
as translation has begun even a little.
E) RNA polymerase
requires a primer to elongate the molecule.
C
In eukaryotes there are several different types of RNA polymerase.
Which type is involved in transcription of mRNA for a globin protein?
A) ligase
B) RNA polymerase I
C) RNA polymerase II
D) RNA polymerase III
E) primase
C
Transcription in eukaryotes requires which of the following in
addition to RNA polymerase?
A) the protein product of the
promoter
B) start and stop codons
C) ribosomes and tRNA
D) several transcription factors (TFs)
E) aminoacyl synthetase
D
What is a ribozyme?
A) an enzyme that uses RNA as a substrate
B) an RNA with enzymatic activity
C) an enzyme that
catalyzes the association between the large and small ribosomal
subunits
D) an enzyme that synthesizes RNA as part of the
transcription process
E) an enzyme that synthesizes RNA primers
during DNA replication
B
In an experimental situation, a student researcher inserts an mRNA
molecule into a eukaryotic cell after he has removed its 5' cap and
poly-A tail. Which of the following would you expect him to find?
A) The mRNA could not exit the nucleus to be translated.
B) The cell recognizes the absence of the tail and
polyadenylates the mRNA.
C) The molecule is digested by
restriction enzymes in the nucleus.
D) The molecule is digested
by exonucleases since it is no longer protected at the 5' end.
E) The molecule attaches to a ribosome and is translated, but
more slowly.
D
Use the following model of a eukaryotic transcript to answer the next
few questions.
5' UTR E₁ I₁ E₂ I₂ E₃ I₃ E₄ UTR 3'
Suppose that an induced mutation removes most of the 5' end of
the 5' UTR. What might result?
A) Removal of the 5' UTR has no
effect because the exons are still maintained.
B) Removal of the
5' UTR also removes the 5' cap and the mRNA will quickly degrade.
C) The 3' UTR will duplicate and one copy will replace the 5'
end.
D) The first exon will not be read because I₁ will now
serve as the UTR.
E) Removal of the 5' UTR will result in the
strand not binding to tRNAs
B
A particular triplet of bases in the coding sequence of DNA is AAA.
The anticodon on the tRNA that binds the mRNA codon is
A) TTT.
B) UUA.
C) UUU.
D) AAA.
E) either UAA or TAA,
depending on first base wobble.
C
What is the function of GTP in translation?
A) GTP energizes
the formation of the initiation complex, using initiation factors.
B) GTP hydrolyzes to provide phosphate groups for tRNA binding.
C) GTP hydrolyzes to provide energy for making peptide bonds.
D) GTP supplies phosphates and energy to make ATP from ADP.
E) GTP separates the small and large subunits of the ribosome at
the stop codon.
A
There are 61 mRNA codons that specify an amino acid, but only 45
tRNAs. This is best explained by the fact that
A) some tRNAs
have anticodons that recognize four or more different codons.
B)
the rules for base pairing between the third base of a codon and tRNA
are flexible.
C) many codons are never used, so the tRNAs that
recognize them are dispensable.
D) the DNA codes for all 61
tRNAs but some are then destroyed.
E) competitive exclusion
forces some tRNAs to be destroyed by nucleases.
B
Which of the following is the first event to take place in
translation in eukaryotes?
A) elongation of the polypeptide
B) base pairing of activated methionine-tRNA to AUG of the
messenger RNA
C) binding of the larger ribosomal subunit to
smaller ribosomal subunits
D) covalent bonding between the first
two amino acids
E) the small subunit of the ribosome recognizes
and attaches to the 5' cap of mRNA
E
What is the function of the release factor (RF)?
A) It
separates tRNA in the A site from the growing polypeptide.
B) It
binds to the stop codon in the A site in place of a tRNA.
C) It
releases the amino acid from its tRNA to allow the amino acid to form
a peptide bond.
D) It supplies a source of energy for
termination of translation.
E) It releases the ribosome from the
ER to allow polypeptides into the cytosol.
B
When the function of the newly made polypeptide is to be secreted
from the cell where it has been made, what must occur?
A) It
must be translated by a ribosome that remains free of attachment to
the ER.
B) Its signal sequence must target it to the ER, from
which it goes to the Golgi.
C) It has a signal sequence that
must be cleaved off before it can enter the ER.
D) It has a
signal sequence that targets it to the cell's plasma membrane where it
causes exocytosis.
E) Its signal sequence causes it to be
encased in a vesicle as soon as it is translated.
B
Why might a point mutation in DNA make a difference in the level of
protein's activity?
A) It might result in a chromosomal
translocation.
B) It might exchange one stop codon for another
stop codon.
C) It might exchange one serine codon for a
different serine codon.
D) It might substitute an amino acid in
the active site.
E) It might substitute the N-terminus of the
polypeptide for the C-terminus.
D
Which small-scale mutation would be most likely to have a
catastrophic effect on the functioning of a protein?
A) a base
substitution
B) a base deletion near the start of a gene
C) a base deletion near the end of the coding sequence, but not
in the terminator codon
D) deletion of three bases near the
start of the coding sequence, but not in the initiator codon
E)
a base insertion near the end of the coding sequence, but not in the
terminator codon
B
Which of the following statements is true about protein synthesis in
prokaryotes?
A) Extensive RNA processing is required before
prokaryotic transcripts can be translated.
B) Translation can
begin while transcription is still in progress.
C) Prokaryotic
cells have complicated mechanisms for targeting proteins to the
appropriate cellular organelles.
D) Translation requires
antibiotic activity.
E) Unlike eukaryotes, prokaryotes require
no initiation or elongation factors.
B
Of the following, which is the most current description of a gene?
A) a unit of heredity that causes formation of a phenotypic
characteristic
B) a DNA subunit that codes for a single complete
protein
C) a DNA sequence that is expressed to form a functional
product: either RNA or polypeptide
D) a DNA—RNA sequence
combination that results in an enzymatic product
E) a discrete
unit of hereditary information that consists of a sequence of amino acids
C
DNA template strand
5' ____________________________ 3'
DNA complementary strand
3' ____________________________
5'
Given the locally unwound double strand above, in
which direction does the RNA polymerase move?
A) 3' → 5' along
the template strand
B) 5' → 3' along the template strand
C) 3' → 5' along the complementary strand
D) 5' → 3' along
the complementary strand
E) 5' → 3' along the double-stranded DNA
A
DNA template strand
5' ____________________________ 3'
DNA complementary strand
3' ____________________________
5'
In the transcription event of the previous DNA, where
would the promoter be located?
A) at the 3' end of the newly
made RNA
B) to the right of the template strand
C) to the
left of the template strand
D) to the right of the sense strand
E) to the left of the sense strand
B
A transfer RNA (#1) attached to the amino acid lysine enters the
ribosome. The lysine binds to the growing polypeptide on the other
tRNA (#2) in the ribosome already.
Which component of the complex described enters the exit tunnel
through the large subunit of the ribosome?
A) tRNA with attached
lysine (#1)
B) tRNA with polypeptide (#2)
C) tRNA that no
longer has attached amino acid
D) newly formed polypeptide
E) initiation and elongation factors
D
Which of the following is not true of a codon?
A) It consists
of three nucleotides.
B) It may code for the same amino acid as
another codon.
C) It never codes for more than one amino acid.
D) It extends from one end of a tRNA molecule.
E) It is
the basic unit of the genetic code
D
Which of the following is not true of RNA processing?
A) Exons
are cut out before mRNA leaves the nucleus.
B) Nucleotides may
be added at both ends of the RNA.
C) Ribozymes may function in
RNA splicing.
D) RNA splicing can be catalyzed by spliceosomes.
E) A primary transcript is often much longer than the final RNA
molecule that leaves the nucleus
A
Which component is not directly involved in translation?
A)
mRNA
B) DNA
C) tRNA
D) ribosomes
E) GTP
B
Which of the following mutations would be most likely to have a
harmful effect on an organism?
A) a nucleotide-pair substitution
B) a deletion of three nucleotides near the middle of a gene
C) a single nucleotide deletion in the middle of an intron
D) a single nucleotide deletion near the end of the coding
sequence
E) a single nucleotide insertion downstream of, and
close to, the start of the coding sequence
E