What does it mean to turn the color blue in the oxidase test?
positive result (presence of cytochrome c oxidase in organism)
What is the name of a medium contains living microbes?
culture/growth medium
Which ID is this?
(idk lol)
What is the technique to isolate colonies with lower cell density samples?
streak plate method
What color are gram-positive cells on the Gram stain?
purple
Which drugs kill the bacteria?
antibiotics
What is the meaning of the I in ELISA test?
immunosorbent
ELISA (enzyme-linked immunosorbent assay)
What is this?
Close up egg with adult parasite
What changes in MacConkey agar to change the color of the agar?
fermentation of lactose (pink to red)
What do you identify in the oxidase test?
bacteria that possess the enzyme cytochrome c oxidase
What structure have the color seen in gram-negative cells?
thin peptidoglycan layer in their cell walls
What is the colloquial name for helminths?
parasitic worms
What do you use to sterilize the rod in the spread plate techniques?
alcohol and bunsen burner flame
What do you use to adjust the bacterial inoculum for turbidity?
McFarland standards
What type is Macconkey agar to lactose fermenters bacteria?
(WTF is this question)
- selective and differential media
- isolate and differentiate gram-negative bacteria based on their ability to ferment lactose
What is this?
helminth egg (this one is trichuris trichiura)
What is being measured?
zone of inhibition
Catalase Test
test used to determine if a bacteria produces the enzyme catalase
What preparation shows us the transparent bacteria?
Staining (simple stain, gram stain)
What is the name of the liquid we use to grow bacteria?
nutrient broth/culture medium
What segment of a worm, contains a complete sexually mature reproductive system.
proglottid
What do you use in hanging drop to form an airtight seal that slows the drying of the drop?
petroleum jelly
Some worms have separate sexes (male/female)
true (roundworms have seperate sexes while majority of other worms are hermaphrodites)
What is this?
proglottid of Taenia saginata
What is the technique to isolate colonies using 4 streaks with high cell density samples?
quadrant streak plate method
What should be done before using the loop to avoid contamination?
flame the loop
What is the name of the process by which competent bacterial cells collect DNA from the environment and use the genes it carries?
transformation
Structure that you saw in the sample
(microscope)
What is the meaning of the L in the ELISA test?
Linked
Order the steps to do the enterotube test
- remove the caps from both ends
- pick a colony with the inoculation wire
- inoculate all compartments
-break off the wire
-punch holes in the foil of the last eight compartments incubate
-interpret the results by comparing colors with the coding pad
When do you need to sterilize the rod in spread plate technique?
the rod or spreader needs to be sterilized before and after use and between streaking sections if multiple sections are used.
What do you see changing in the ELISA test if it is positive?
color change in the wells
What are the instances that allow a substance (germicide) to be registered and allowed on the market?
if it passes regulatory requirements set by agencies like the EPA and FDA, including submitting data on its efficacy, safety, and environmental impact
Which drug stops the bacteria from dividing but does not kill them?
bacteriostatic antibiotic
What is the meaning of the A in ELISA test?
Assay