Primase
enzyme that lays down an RNA primer on both the leading and lagging strands during replication
DNA polymerase
enzyme that adds or "zips up" nucleotides to both leading and lagging strands of DNA during replication,
Primer
starting point of replication on both leading and lagging strand with temporary RNA nucleotides, this will be replaced with actual DNA nucleotides by DNA polymerase
semi-conservative
term to describe how DNA replication is one new strand with one original strand
What did Watson and Crick discover?
structure of DNA (with the help of Franklin)
anti-parallel
term to describe how the DNA strands run opposite direction
termination signal
place on the DNA where transcription stops
Where does both replication and transcription occur?
nucleoplasm (in nucleus)
ligase
enzyme that "seals up" spaces between Okazaki fragments on the lagging strand during replication
Okazaki fragments
chunks/pieces of replication on the lagging strand
3 steps of transcription
initiation, elongation, termination
start codon
AUG
What is the shape of Watson and Crick model of DNA ?
double helix
What is the sugar in DNA?
Deoxyribose
Promoter
place on DNA where transcription starts
The order of nitrogen bases in DNA determines the order of what in proteins?
AA
anti-codon
3 nitrogen base code on the tRNA,
Nucleotide
a building block of DNA or RNA (nucleic acids)
phosphodiester covalent
strong bonds in DNA on the sides
sequence
the order of nucleotides in DNA or RNA or the order of amino acids in a protein.
ribose
the sugar of RNA
Transcription
the process of giving DNA's information to mRNA
Translation
the process that turns the RNA language into the protein language by the mRNA and tRNA meeting up at the rRNA ribosome
Central dogma
the concept that genetic information encoded in DNA is transcribed to mRNA. Then the mRNA moves to the ribosome where the information is translated into the amino acid sequnce of a protein (DNA->RNA->Protein).
AA
a building block of proteins
Mutation
a change in the nucleotide base sequence of a gene or DNA molecule.
codon
The three-nucleotide sequence on mRNA
helicase
enzyme that unzips strands of DNA by breaking H bonds during replication
single-stranded binding proteins
their job is to keep the 2 strands of DNA separated while replication occurs
RNA polymerase
enzyme that both unzips AND zips up during transcription
gene
A stretch of chromosome/DNA that codes for a trait
A nucleotide consists
a sugar, a phosphate, and a nitrogen base
replication
The process by which DNA makes a copy of itsel
dipeptide
two amino acids bonded together after translation
polypeptide chain
3 or more amino acids
3 main processes in this chapter
replication, transcription, translation
orientation of twist of DNA helix
right-handed turn
purines
adenine and guanine; 2 rings of carbon
pyrimidines
cytosine and thymine; 1 ring of carbon
replication fork
the Y-shaped region that results when the 2 strands separate
replication origin bubbles
several points along DNA double-strands that replicate at the same time (to save time)
mRNA
first type of RNA that carries the instructions from DNA to the ribosome to make a protein
rRNA
part of the stucture of ribosome, which is an organelle where protein synthesis occurs
tRNA
third type of RNA that transfers amino acids to the ribosome to make a protein
genome
the complete genetic material contained in an individual
hydrogen bond
weak bonds in DNA in the middle
Rosalind Franklin
Woman who generated x-ray images of DNA, she povided Watson and Crick with key data about DNA
Watson and Crick
discovered the structure of DNA
leading strand
in replication, the new DNA strand synthesized continuously along the template strand toward the replication fork in the mandatory 5' to 3' direction
lagging strand
in replication, the strand that is synthesized in fragments using individual sections
What are the two steps of protein synthesis?
transcription and translation
3 stop codons
UAA, UAG, UGA
start codon
place on mRNA that starts translation
stop codon
place on mRNA that stops translation
Where does translation occur?
ribosome
topoisomerase
enzyme that unwinds/untwists the strands of DNA during replication