BICH EXAM 2: Electron transport and Oxidative Phosphorylation Flashcards

Electron transport is when electrons are carried by reduced coenzyme (NADH and FADH₂) are passed through a _______ and conenzymes to drive the generation of a proton gradient across the _________

The proton gradient drives the synthesis of ATP

The complexes allow for the flow of

(Δℰo‘ = ℰo‘ (acceptor) - ℰo‘(donor)) .

Complex 1 makes

Complex 3 makes

Complex 4 makes

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Complex I: NADH-UQ reductase

NADH-coenzyme Q oxidoreductase pumps

Complex 1 is reduced by_____, and oxidized by______

Ubiquinone contains no

Ubisemiquinone contains

Ubiquinol contains

Complex 1 transports H⁺ from

__H⁺ are transported from the matrix across the _____membrane to the cytosolic side

Is a component of e- transport complex 2

Complex 2 utilized Fe-S clusters and hemes to move electrons from

No proton transport occurs at complex 2 as electrons are

Starts at coenzyme Q and ends at cytochrome C oxidoreductase

Complex 3 results in QH₂ is _____, and ______by Cyt C.

In complex 3, QH_{2_____}electrons, and Cyt C_____electrons from complex 3.

CoQ passes electrons to cyt c in a redox cycle known as

The principle transmembrane in complex 3 is the

The path electrons take through complex 3

1. UQH2-> Rieske Fe-S-> Cyt C (release 2H+, leaves UQ)

2. 1e- transferred from UQ. to b_L heme then to b_H heme

1. 2nd UQH2 is oxidized same way as first half

2. However after electron is transferred to b_h heme, it is transferred to QH.- to reform UQH2 (release 2 H+

Cytochrome c is a

Cytochrome c is the only mitochondrial cytochrome that is _____soluable, important because it shuttles electrons from complex 3 to complex 4 by _____associating with the inner mitochondrial membrane

Complex 4 cytochrome C oxidase pumps

Complex 4 is reduced by____ and oxidized by______. Electron carriers are ______ and______

Free radicals are

.OH (hydroyl) and O₂.- (superoxide radicals)

Complex 4 transfers electrons in ________. This shields the rest of the cell from____

What is the result of the oxidation of NADH and FADH₂?

This mechanism stores the energy of electron transport in an electrochemical potential: pH rises and matrix becomes

Protons are highly attracted back to the interior of the matrix and the energetically favorable electrochemical

NADH has ___ATP transport of H+

FADH₂ has _____ATP transport of H+

H+ will be pumped from matrix to IMS if rotating

Rotation of the c-ring delivers protons to the outlet

Flow of protons through structure turns the rotor and drives cycle of conformational changes in

Aspartic acid is buried in a ______ core, where proton cannot be released.

Each 120 degree rotation equal

Each 3x120 degrees so,

KNow thissss

ATP must be transported out of

ATP is ____ and ADP is ___ in translocase

ATP movement out is favored because the cytosol is + relative to the

So every ATP transported costs

1ATP=